2012 Vol. 30, No. 1
Display Method:
2012, 30(1): 1-3,13.
doi: 10.3969/j.issn.1006-0111.2012.01.001
Abstract:
The IPSC technology, which was discovered in 2006, had drawn much attention among laboratories around the world and become a new milestone in the life science. The history of pluripotency study, with an emphasis on the culturing technology of IPSC and its potential applications in cell therapy was reviewed. According to the large amount of reviews and articles, the progress that had been made in the IPSC field in recent years in hope to provide some references for the subsequent research.
The IPSC technology, which was discovered in 2006, had drawn much attention among laboratories around the world and become a new milestone in the life science. The history of pluripotency study, with an emphasis on the culturing technology of IPSC and its potential applications in cell therapy was reviewed. According to the large amount of reviews and articles, the progress that had been made in the IPSC field in recent years in hope to provide some references for the subsequent research.
2012, 30(1): 4-7,57.
doi: 10.3969/j.issn.1006-0111.2012.01.002
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Both the stealth and targeting features were important for effective and selective drug delivery to the tumors.However, it was difficult to achieve both features simultaneously. Some of the novel targeting strategies had the potential to overcome this challenge. The new targeting technologies with recent examples were reviewed, which utilized the environmental stimuli to activate the nanocarriers. Traditional strategies for tumor-targeted nanocarriers were briefly discussed with an emphasis on their achievements and challenges.
Both the stealth and targeting features were important for effective and selective drug delivery to the tumors.However, it was difficult to achieve both features simultaneously. Some of the novel targeting strategies had the potential to overcome this challenge. The new targeting technologies with recent examples were reviewed, which utilized the environmental stimuli to activate the nanocarriers. Traditional strategies for tumor-targeted nanocarriers were briefly discussed with an emphasis on their achievements and challenges.
2012, 30(1): 8-13.
doi: 10.3969/j.issn.1006-0111.2012.01.003
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Objective To review the chemical and pharmacological study of the C6-C3 compounds from genus Illicium and provide the reference for further development of genus Illicium. Methods Relevant literatures of C6-C3 compounds in genus Illicium were searched and summarized. Results and Conclusion The plants of this genus had varying types C6-C3 compounds which had good anti-inflammatory, neurotrophic, anti-cancer and anti-oxidation activities. The C6-C3 compounds in this genus had broad prospects for medicinal development.
Objective To review the chemical and pharmacological study of the C6-C3 compounds from genus Illicium and provide the reference for further development of genus Illicium. Methods Relevant literatures of C6-C3 compounds in genus Illicium were searched and summarized. Results and Conclusion The plants of this genus had varying types C6-C3 compounds which had good anti-inflammatory, neurotrophic, anti-cancer and anti-oxidation activities. The C6-C3 compounds in this genus had broad prospects for medicinal development.
2012, 30(1): 14-18,37.
doi: 10.3969/j.issn.1006-0111.2012.01.004
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Peptide analog of hypoglycemic drugs had a normal physiological function of insulin secretion. The hypoglycemic effect of peptide depended on blood glucose levels of patients, which induced very few incidents of hypoglycemia. Peptides of sustained and controlled release delivery systems in diabetes treatment were summed up in this paper.
Peptide analog of hypoglycemic drugs had a normal physiological function of insulin secretion. The hypoglycemic effect of peptide depended on blood glucose levels of patients, which induced very few incidents of hypoglycemia. Peptides of sustained and controlled release delivery systems in diabetes treatment were summed up in this paper.
2012, 30(1): 19-24,41.
doi: 10.3969/j.issn.1006-0111.2012.01.005
Abstract:
The small organic molecules catalyst was a hotspot in asymmetric synthesis. Due to the excellent enantiselectivity of proline in many asymmetric reactions, people showed great interest in the modification of it, which aimed to get better organocatalysts. Proline derivatives were divided into ten kinds and reviewed the progression in asymmetric reactions according to these types in this paper.
The small organic molecules catalyst was a hotspot in asymmetric synthesis. Due to the excellent enantiselectivity of proline in many asymmetric reactions, people showed great interest in the modification of it, which aimed to get better organocatalysts. Proline derivatives were divided into ten kinds and reviewed the progression in asymmetric reactions according to these types in this paper.
2012, 30(1): 25-28,31.
doi: 10.3969/j.issn.1006-0111.2012.01.006
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Objective To investigate the protect effect and mechanism of total polysaccharides (TPC) isolated from 50% ethanol extract of Wuzi-Yanzong pill (WZ) against chronic alcohol-induced injury in Rat . Methods The effect of TPC on chronic ethanol hepatotoxicity was investigated in modified Lieber-DeCarli rat model. Hepatocellular damage indicator (Serum ALT, AST) and histopathological examination of liver in rat were evaluated. MDA content, SOD activity and GSH content in liver were measured. TNF-α content and TNF-α mRNA expression were determined. Results TPC protected against chronic ethanol-induced hepatotoxicity in rat, as evidenced by decreased plasma transaminases activities and signally improve the liver pathological changes induced by alcohol. TPC significantly inhibited the increase of MDA content, improved SOD activity and prevented GSH depletion. TPC significantly alleviated hepatic TNF-α production and decreased the expression of TNF-α mRNA. Conclusion TPC possessed potent hepatoprotective activities in chronic alcohol-induced injury rat model. The suppressive effect of TPC on chronic alcohol induced liver injury might be attributed to the antioxidant action and suppress TNF-α expression at protein and gene level.
Objective To investigate the protect effect and mechanism of total polysaccharides (TPC) isolated from 50% ethanol extract of Wuzi-Yanzong pill (WZ) against chronic alcohol-induced injury in Rat . Methods The effect of TPC on chronic ethanol hepatotoxicity was investigated in modified Lieber-DeCarli rat model. Hepatocellular damage indicator (Serum ALT, AST) and histopathological examination of liver in rat were evaluated. MDA content, SOD activity and GSH content in liver were measured. TNF-α content and TNF-α mRNA expression were determined. Results TPC protected against chronic ethanol-induced hepatotoxicity in rat, as evidenced by decreased plasma transaminases activities and signally improve the liver pathological changes induced by alcohol. TPC significantly inhibited the increase of MDA content, improved SOD activity and prevented GSH depletion. TPC significantly alleviated hepatic TNF-α production and decreased the expression of TNF-α mRNA. Conclusion TPC possessed potent hepatoprotective activities in chronic alcohol-induced injury rat model. The suppressive effect of TPC on chronic alcohol induced liver injury might be attributed to the antioxidant action and suppress TNF-α expression at protein and gene level.
2012, 30(1): 29-31.
doi: 10.3969/j.issn.1006-0111.2012.01.007
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Objective To develop a fast reverse-phase high-performance liquid chromatography assay method for the determination of ambroxol hydrochloride. Methods A Diamonsil-C18(200 mm×4.6 mm,5μm)column was used.The mobile phase consisted of methanol-0.01 mol/L PBS(pH 7.0)-THF(700:320:25,v/v/v).Detection wavelength was 242 nm. Results A good linear relationship was obtained in the concentration range of 10.2~610.8 ng/ml. The average recovery of this method was 88.2％~104.8％. The RSD of within-day and between-day were all less than 15％. Conclusion The method was simple,rapid and accurate. The result showed that the method was suitable for the determination of ambroxol hydrochloride.
Objective To develop a fast reverse-phase high-performance liquid chromatography assay method for the determination of ambroxol hydrochloride. Methods A Diamonsil-C18(200 mm×4.6 mm,5μm)column was used.The mobile phase consisted of methanol-0.01 mol/L PBS(pH 7.0)-THF(700:320:25,v/v/v).Detection wavelength was 242 nm. Results A good linear relationship was obtained in the concentration range of 10.2~610.8 ng/ml. The average recovery of this method was 88.2％~104.8％. The RSD of within-day and between-day were all less than 15％. Conclusion The method was simple,rapid and accurate. The result showed that the method was suitable for the determination of ambroxol hydrochloride.
2012, 30(1): 32-34.
doi: 10.3969/j.issn.1006-0111.2012.01.008
Abstract:
Objective To study the preparation method of quercetin liposome and screen the optimal technological conditions by the particle sizes and the encapsulation efficiencies of quercetin. Method Liposomes were made of hydrogenated soybean phosphatidylcholine(HSPC) and cholesterol(CH) by film evaporation and probe ultrasonic technique, then orthogonal design was adopted to screen the optimal conditions. The particle sizes were detected by Zetasizer Nano, while the encapsulation efficiency of quercetin (QU) were determined by HPLC after the free drug was separated by protamine sedimentation method. Results The optimal technological conditions of quercetin liposome were as follows: HSPC∶CH=3∶1, HSPC:QU=20:1, the time of probe ultrasonic (600 w) was 9 minutes. Conclusion Film evaporation and probe ultrasonic technique could be suitable for laboratory to prepare quercetin liposome.
Objective To study the preparation method of quercetin liposome and screen the optimal technological conditions by the particle sizes and the encapsulation efficiencies of quercetin. Method Liposomes were made of hydrogenated soybean phosphatidylcholine(HSPC) and cholesterol(CH) by film evaporation and probe ultrasonic technique, then orthogonal design was adopted to screen the optimal conditions. The particle sizes were detected by Zetasizer Nano, while the encapsulation efficiency of quercetin (QU) were determined by HPLC after the free drug was separated by protamine sedimentation method. Results The optimal technological conditions of quercetin liposome were as follows: HSPC∶CH=3∶1, HSPC:QU=20:1, the time of probe ultrasonic (600 w) was 9 minutes. Conclusion Film evaporation and probe ultrasonic technique could be suitable for laboratory to prepare quercetin liposome.
2012, 30(1): 35-37.
doi: 10.3969/j.issn.1006-0111.2012.01.009
Abstract:
Objective To optimize the synthesis of substituted tetrahydronaphthalene and investigate the influence of 10% Pd/C catalyst in the synthesis of key intermediate. Methods A parallel test was carried out to compare the catalytic activity of triethyl silicane and the 10% Pd/C catalyst, including the total analysis of experimental result . And orthogonal experimental design was used and the influence of solvent and reactant ratio on the yield were investigated. Results The cost of the 10% Pd/C catalyst was obviously lower than that of triethyl silicane,with high yield, little impurity easy effluent disposal. Orthogonal test confirms that the ratio of reactant and catalystthe was the most significant factor. Conclusion The experiments proved that the optimal preparation procedure was much more available for industrial production using the 10% Pd/C as a catalyst.
Objective To optimize the synthesis of substituted tetrahydronaphthalene and investigate the influence of 10% Pd/C catalyst in the synthesis of key intermediate. Methods A parallel test was carried out to compare the catalytic activity of triethyl silicane and the 10% Pd/C catalyst, including the total analysis of experimental result . And orthogonal experimental design was used and the influence of solvent and reactant ratio on the yield were investigated. Results The cost of the 10% Pd/C catalyst was obviously lower than that of triethyl silicane,with high yield, little impurity easy effluent disposal. Orthogonal test confirms that the ratio of reactant and catalystthe was the most significant factor. Conclusion The experiments proved that the optimal preparation procedure was much more available for industrial production using the 10% Pd/C as a catalyst.
2012, 30(1): 38-41.
doi: 10.3969/j.issn.1006-0111.2012.01.010
Abstract:
Objective To investigate the preparation method, the structure and the sun protection factor (SPF) of the polyelectrolyte self-assembled microcapsules containing TiO2 nanoparticles. Methods Calcium carbonate was used as the core, and the semipermeable membrane outside the core was made of poly (sodium 4-styrenesulfonate) (PSS) and TiO2 depositing onto the core surface alternately through electrostatic attraction. The morphology and the structure of the microcapsules were obtained by confocal laser scanning microscopy and scanning electron microscopy. The SPF was tested by SPF-290 analytical system. Results The prepared microcapsules were well-shaped with a mean diameter of (5.46±1.05) μm. The SPF was 30.31±2.56. Conclusion It was indicated that in comparison with traditional TiO2 sunscreen cream, the polyelectrolyte microcapsules containing titania had powerful absorbance of the ultraviolet rays.
Objective To investigate the preparation method, the structure and the sun protection factor (SPF) of the polyelectrolyte self-assembled microcapsules containing TiO2 nanoparticles. Methods Calcium carbonate was used as the core, and the semipermeable membrane outside the core was made of poly (sodium 4-styrenesulfonate) (PSS) and TiO2 depositing onto the core surface alternately through electrostatic attraction. The morphology and the structure of the microcapsules were obtained by confocal laser scanning microscopy and scanning electron microscopy. The SPF was tested by SPF-290 analytical system. Results The prepared microcapsules were well-shaped with a mean diameter of (5.46±1.05) μm. The SPF was 30.31±2.56. Conclusion It was indicated that in comparison with traditional TiO2 sunscreen cream, the polyelectrolyte microcapsules containing titania had powerful absorbance of the ultraviolet rays.
2012, 30(1): 42-44,48.
doi: 10.3969/j.issn.1006-0111.2012.01.011
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Objective To establish a method for determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in plasma of SD rats by RP-HPLC. Methods The chromatographic separation was performed on a Diamonsil-C18 column(100 mm×4.6 mm,5 μm) with the mobile phase consisting of acetonitrile/5 mM ammonium acetate buffer(pH 4.0)(30/70,v/v). The single intravenous injection of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate was given to 70 SD rats to study the pharmacokinetics. Results The intra-run and inter-run RSD were less than 15%. The pharmacokinetics parameters were as follows: Cmax was 35.60 μg/ml , t1/2 was 3.36 h, AUC0~12 was 17.12 μg h/ml, AUC0~∞ was 17.72μg h/ml. Conclusion The method was simple and accurate, which could be used to separation and determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate.
Objective To establish a method for determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate in plasma of SD rats by RP-HPLC. Methods The chromatographic separation was performed on a Diamonsil-C18 column(100 mm×4.6 mm,5 μm) with the mobile phase consisting of acetonitrile/5 mM ammonium acetate buffer(pH 4.0)(30/70,v/v). The single intravenous injection of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate was given to 70 SD rats to study the pharmacokinetics. Results The intra-run and inter-run RSD were less than 15%. The pharmacokinetics parameters were as follows: Cmax was 35.60 μg/ml , t1/2 was 3.36 h, AUC0~12 was 17.12 μg h/ml, AUC0~∞ was 17.72μg h/ml. Conclusion The method was simple and accurate, which could be used to separation and determination of (E)-3-(3-methoxy-4-((3,5,6-trimethylpyrazin-2-yl) methoxy)phenyl)acrylate.
2012, 30(1): 45-48.
doi: 10.3969/j.issn.1006-0111.2012.01.012
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Objective To establish a LC-MS/MS method for determination of polydatin concentration in rats plasma. Methods The plasma concentration of polydatin was determined by LC-MS/MS. Using stilbene glucoside as internal standard, after precipitation of the plasma proteins with methanol, the analytes were separated on an agilent zorbax SB-C18 reversed-phase column with methanol-acetonitrile- 0.1% formic acid (18 : 15 : 67, v/v) and detected by electrospray ionization (ESI) mass spectrometry in negative multiple reaction monitoring (MRM) mode. The flow rate was 0.3 ml/min. Column temperature was maintained at 30 ℃. Results The calibration curves with good linearity (r=0.998 4 for plasma sample) were obtained in the range of 1.0~5000.0 ng/ml for polydatin. The lower limit of quantification (LLOQ) was 1.0 ng/ml. Recoveries were around 78% for the extraction from rats plasma, and good precision and accuracy were achieved. The intra-day and inter-day RSD were both less than 15.0%. This method was feasible for the evaluation of pharmacokinetic profiles of polydatin in rats. Conclusion This method was simple, selective and sensitive. It's suitable for the pharmacokinetic research of polydatin in rats plasma.
Objective To establish a LC-MS/MS method for determination of polydatin concentration in rats plasma. Methods The plasma concentration of polydatin was determined by LC-MS/MS. Using stilbene glucoside as internal standard, after precipitation of the plasma proteins with methanol, the analytes were separated on an agilent zorbax SB-C18 reversed-phase column with methanol-acetonitrile- 0.1% formic acid (18 : 15 : 67, v/v) and detected by electrospray ionization (ESI) mass spectrometry in negative multiple reaction monitoring (MRM) mode. The flow rate was 0.3 ml/min. Column temperature was maintained at 30 ℃. Results The calibration curves with good linearity (r=0.998 4 for plasma sample) were obtained in the range of 1.0~5000.0 ng/ml for polydatin. The lower limit of quantification (LLOQ) was 1.0 ng/ml. Recoveries were around 78% for the extraction from rats plasma, and good precision and accuracy were achieved. The intra-day and inter-day RSD were both less than 15.0%. This method was feasible for the evaluation of pharmacokinetic profiles of polydatin in rats. Conclusion This method was simple, selective and sensitive. It's suitable for the pharmacokinetic research of polydatin in rats plasma.
2012, 30(1): 49-51,63.
doi: 10.3969/j.issn.1006-0111.2012.01.013
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Objective To establish a HPLC method to determine the content and related substance of rosiglitazone hydrochloride tablets. Methods The analysis was achieved on a Diamonsil C18 column(250 mm×4.6 mm, 5 μm) using a mobile phase of 0.025 mol/L ammonium acetate-acetonitrile (50∶50). The detection wavelength was at 245 nm. Results There was good resolution among the peak of rosiglitazone, the main related substances and the degradable impurities after being destroyed. There was a good linear relationship between the peak area and the concentration of rosiglitazone in the range of 19.57~195.70 μg/ml (r=1.000 0, n=6). Regression equation could be expressed by A=68 525C+31 152, r=1.000 0 (n=6). The precision in a day (RSD﹤0.5%) and during the days (RSD﹤1.0%) were good. The average recovery was 99.8% (RSD=0.3%,n=9) and the limit of detection was 0.29 ng. The solution was stable for 10 hours. Conclusion The method was specific, accurate, sensitive and could be used to determine rosiglitazone.
Objective To establish a HPLC method to determine the content and related substance of rosiglitazone hydrochloride tablets. Methods The analysis was achieved on a Diamonsil C18 column(250 mm×4.6 mm, 5 μm) using a mobile phase of 0.025 mol/L ammonium acetate-acetonitrile (50∶50). The detection wavelength was at 245 nm. Results There was good resolution among the peak of rosiglitazone, the main related substances and the degradable impurities after being destroyed. There was a good linear relationship between the peak area and the concentration of rosiglitazone in the range of 19.57~195.70 μg/ml (r=1.000 0, n=6). Regression equation could be expressed by A=68 525C+31 152, r=1.000 0 (n=6). The precision in a day (RSD﹤0.5%) and during the days (RSD﹤1.0%) were good. The average recovery was 99.8% (RSD=0.3%,n=9) and the limit of detection was 0.29 ng. The solution was stable for 10 hours. Conclusion The method was specific, accurate, sensitive and could be used to determine rosiglitazone.
2012, 30(1): 52-54.
doi: 10.3969/j.issn.1006-0111.2012.01.014
Abstract:
Objective To establish a HPLC-MS/MS method for determination of sodium Danshensu in SD rat liver. Methods Tissue samples were pretreated by liquid-liquid extraction method after homogenate, and ketoprofen was used as the internal standard. Analytical column was Diamonsil C18 (200 mm×4.6 mm, 5 μm). The mobile phase was methanol: water(0.01‰ formic acid)=80:20 with flow rate as 1.0 ml/min. Mass spectrum determination was performed in the MRM mode with target ions m/z 197→135 (Sodium Danshensu), m/z 253→209 (ketoprofen), using ESI ion resource and negative ion detection. Results The calibration curve was linear over the range of 20.2~1 010 ng/ml. The LLOQ of sodium Danshensu in liver was 20.2 ng/ml. The intra-and inter-day RSD were both less than 10%. The extracted recovery rate ranged from 92% to 107%. Conclusion The method was sensitive, rapid, simple and accurate to determine sodium Danshensu distribution in rat liver and was suitable for preclinical test of Sodium Danshensu.
Objective To establish a HPLC-MS/MS method for determination of sodium Danshensu in SD rat liver. Methods Tissue samples were pretreated by liquid-liquid extraction method after homogenate, and ketoprofen was used as the internal standard. Analytical column was Diamonsil C18 (200 mm×4.6 mm, 5 μm). The mobile phase was methanol: water(0.01‰ formic acid)=80:20 with flow rate as 1.0 ml/min. Mass spectrum determination was performed in the MRM mode with target ions m/z 197→135 (Sodium Danshensu), m/z 253→209 (ketoprofen), using ESI ion resource and negative ion detection. Results The calibration curve was linear over the range of 20.2~1 010 ng/ml. The LLOQ of sodium Danshensu in liver was 20.2 ng/ml. The intra-and inter-day RSD were both less than 10%. The extracted recovery rate ranged from 92% to 107%. Conclusion The method was sensitive, rapid, simple and accurate to determine sodium Danshensu distribution in rat liver and was suitable for preclinical test of Sodium Danshensu.
2012, 30(1): 55-57.
doi: 10.3969/j.issn.1006-0111.2012.01.015
Abstract:
Objective To investigate the effect of combination of Compound Chuanqiong capsules and irbesartan in patients with diabetic nephropathy. Methods 323 patients with diabetic nephropathy were randomly divided into 2 groups. Group I (Compound chuanqiong capsules plus Irbesartan group,n=161)and Group II (Irbesartan group,n=162). After 6 months follow-up,cystatin C,serum creatinine,24 h urine protein quantitate were respectively detected and compared before and after treatment. Results Compared to patients in the control group cystatin C,the patients'serum creatinine,24 h urine protein quantities decreased (P＜0.05). Conclusion The combination of Compound Chuanqiong capsules and irbesartan was more effective than irbesartan alone in the treatment of diabetic nephropathy.
Objective To investigate the effect of combination of Compound Chuanqiong capsules and irbesartan in patients with diabetic nephropathy. Methods 323 patients with diabetic nephropathy were randomly divided into 2 groups. Group I (Compound chuanqiong capsules plus Irbesartan group,n=161)and Group II (Irbesartan group,n=162). After 6 months follow-up,cystatin C,serum creatinine,24 h urine protein quantitate were respectively detected and compared before and after treatment. Results Compared to patients in the control group cystatin C,the patients'serum creatinine,24 h urine protein quantities decreased (P＜0.05). Conclusion The combination of Compound Chuanqiong capsules and irbesartan was more effective than irbesartan alone in the treatment of diabetic nephropathy.
2012, 30(1): 58-59.
doi: 10.3969/j.issn.1006-0111.2012.01.016
Abstract:
Objective To explore the application of moist burn ointment in skin injury caused by diarrhea in patients of ICU and solve the problems of perianal skin care in ICU diarrhea patients. Method According to the admission sequence,ICU patients was divided into observation group and control group, The anus, perineum of each patient were cleaned by warm boiling water after diarrhea. The patients with skin ulceration were cleaned with saline cotton and PVP iodine cotton ball. The control group was used the traditional infrared irradiation, the observation group was treated with moist burn ointment. Result The healing rate of the observation group was significantly higher than the control group. Conclusion The effect of MEBO in treatment of perianal skin injury patients caused by severe diarrhea was good and convenient, which was worth promoting in clinic.
Objective To explore the application of moist burn ointment in skin injury caused by diarrhea in patients of ICU and solve the problems of perianal skin care in ICU diarrhea patients. Method According to the admission sequence,ICU patients was divided into observation group and control group, The anus, perineum of each patient were cleaned by warm boiling water after diarrhea. The patients with skin ulceration were cleaned with saline cotton and PVP iodine cotton ball. The control group was used the traditional infrared irradiation, the observation group was treated with moist burn ointment. Result The healing rate of the observation group was significantly higher than the control group. Conclusion The effect of MEBO in treatment of perianal skin injury patients caused by severe diarrhea was good and convenient, which was worth promoting in clinic.
2012, 30(1): 60-60,66.
doi: 10.3969/j.issn.1006-0111.2012.01.017
Abstract:
Objective Summary Batroxobin injection combined Ginkgo biloba extract and dipyridamole injection efficacy of treatment of sudden deafness,Treatment of sudden deafness of effective drug. Method Selected 108 cases (120 ears) in patients with sudden deafness diagnosis,Randomly divided into treatment and control groups,The treatment for 2 weeks. Result Treatment group was 85%,Control group was 66%,Two groups was highly significant difference (P<0.05),No serious adverse reactions and complications. Conclusion Batroxobin injection combined Ginkgo biloba extract and dipyridamole injection assisted treatment of sudden deafness results were satisfactory.
Objective Summary Batroxobin injection combined Ginkgo biloba extract and dipyridamole injection efficacy of treatment of sudden deafness,Treatment of sudden deafness of effective drug. Method Selected 108 cases (120 ears) in patients with sudden deafness diagnosis,Randomly divided into treatment and control groups,The treatment for 2 weeks. Result Treatment group was 85%,Control group was 66%,Two groups was highly significant difference (P<0.05),No serious adverse reactions and complications. Conclusion Batroxobin injection combined Ginkgo biloba extract and dipyridamole injection assisted treatment of sudden deafness results were satisfactory.
2012, 30(1): 61-63.
doi: 10.3969/j.issn.1006-0111.2012.01.018
Abstract:
Objective To Sum up the situation of using antibiotics in intracoronary coronary stenting, and normalize the usage criterion and reinforce the management of antibiotics. Methods Retrospective survey method was used. 206 cases of patients who underwent cardiac intervention surgery between Jan., 2011 to May, 2011 were randomly selected for investigating and analyzing the usage of antibiotics. Results In the 260 cases, only 7 cases received no antibacterial treatment, while other 199 cases (96.6%) received prophylactic antibacterial treatment. The cephamycins was the predominant prophylactic antibiotics with the utility rate of 60.86%; the usage rate of first-generation and second-generation cephalosporins were 16.21% and 13.04% respectively; the usage rate of quinolone was 5.53%. All cases were medicated after surgery. The duration of prophylactic therapy spanned from 1d to 12 d, and the average antibacterial usage period was 3 d. Conclution The outstanding problems in prophylactic usage of antibiotics were the improper choice for the antibiotics, the improper choice for the medicinal usage occasion and the prolonged postoperative usage of antibiotics. It was urgent to intensify physicians' consciousness about rational use of antibiotics in intracoronary coronary stenting during perioperative period.
Objective To Sum up the situation of using antibiotics in intracoronary coronary stenting, and normalize the usage criterion and reinforce the management of antibiotics. Methods Retrospective survey method was used. 206 cases of patients who underwent cardiac intervention surgery between Jan., 2011 to May, 2011 were randomly selected for investigating and analyzing the usage of antibiotics. Results In the 260 cases, only 7 cases received no antibacterial treatment, while other 199 cases (96.6%) received prophylactic antibacterial treatment. The cephamycins was the predominant prophylactic antibiotics with the utility rate of 60.86%; the usage rate of first-generation and second-generation cephalosporins were 16.21% and 13.04% respectively; the usage rate of quinolone was 5.53%. All cases were medicated after surgery. The duration of prophylactic therapy spanned from 1d to 12 d, and the average antibacterial usage period was 3 d. Conclution The outstanding problems in prophylactic usage of antibiotics were the improper choice for the antibiotics, the improper choice for the medicinal usage occasion and the prolonged postoperative usage of antibiotics. It was urgent to intensify physicians' consciousness about rational use of antibiotics in intracoronary coronary stenting during perioperative period.
2012, 30(1): 64-66.
doi: 10.3969/j.issn.1006-0111.2012.01.019
Abstract:
Objective To evaluate the roles of the two indexes, defined single dose (DSD) and defined daily frequency (DDF), in the rational use of antimicrobial drugs in inpatients of respiratory department. Methods 20 percent of cases of respiratory department were selected from 2010. IF condition function in Microsoft Excel Software was used to evaluate if the single dose and daily frequency of each prescription were within the defined range.Results By the method based on DSD and DSF, there were thee cases of irrational single dose, twenty cases of irrational frequency, five cases of both irrational single dose and frequency. Conclusion Compared with the DDDs, the method based on DSD and DDF was more efficient, with fewer errors, and was more suitable for rational use evaluation of large quantities of antimicrobial agents in inpatient department.
Objective To evaluate the roles of the two indexes, defined single dose (DSD) and defined daily frequency (DDF), in the rational use of antimicrobial drugs in inpatients of respiratory department. Methods 20 percent of cases of respiratory department were selected from 2010. IF condition function in Microsoft Excel Software was used to evaluate if the single dose and daily frequency of each prescription were within the defined range.Results By the method based on DSD and DSF, there were thee cases of irrational single dose, twenty cases of irrational frequency, five cases of both irrational single dose and frequency. Conclusion Compared with the DDDs, the method based on DSD and DDF was more efficient, with fewer errors, and was more suitable for rational use evaluation of large quantities of antimicrobial agents in inpatient department.
2012, 30(1): 67-69,77.
doi: 10.3969/j.issn.1006-0111.2012.01.020
Abstract:
Objective To grasp the domestic hospitals in the present situation of the pharmacy trusteeship,and explore the model of separation of prescribing and dispensing. Methods The advantages and disadvantages of the hospital pharmacy trusteeship were analyzed through literature research. Results The advantages and disadvantages of the hospital pharmacy trusteeship were expounded, and the optimized methods of hospital pharmacy trusteeship were put forward. Conclusion Medical institutions could learn from the experience of hospital pharmacy trusteeship, and optimize the hospital pharmacy management mode so as to gradually realize the separation of prescribing and dispensing.
Objective To grasp the domestic hospitals in the present situation of the pharmacy trusteeship,and explore the model of separation of prescribing and dispensing. Methods The advantages and disadvantages of the hospital pharmacy trusteeship were analyzed through literature research. Results The advantages and disadvantages of the hospital pharmacy trusteeship were expounded, and the optimized methods of hospital pharmacy trusteeship were put forward. Conclusion Medical institutions could learn from the experience of hospital pharmacy trusteeship, and optimize the hospital pharmacy management mode so as to gradually realize the separation of prescribing and dispensing.
2012, 30(1): 70-72.
doi: 10.3969/j.issn.1006-0111.2012.01.021
Abstract:
Objective To analyze the prescriptions of drug return and explore the reason and treatment of drug return phenomenon from outpatient pharmacy. To propose appropriate countermeasures to reduce the incidence of drug return and promote rational drug use. Methods 50 prescriptions of drug return were collected from outpatient pharmacy in 3 years. The reason of drug return were statistical analyzed. Results The reason of drug return from outpatient pharmacy was mainly attributable to the change of patient's condition, adverse reaction, and other factors from patients and doctors. Conclusion The incidence of drug return could be reduced effectively through relevant precautions.
Objective To analyze the prescriptions of drug return and explore the reason and treatment of drug return phenomenon from outpatient pharmacy. To propose appropriate countermeasures to reduce the incidence of drug return and promote rational drug use. Methods 50 prescriptions of drug return were collected from outpatient pharmacy in 3 years. The reason of drug return were statistical analyzed. Results The reason of drug return from outpatient pharmacy was mainly attributable to the change of patient's condition, adverse reaction, and other factors from patients and doctors. Conclusion The incidence of drug return could be reduced effectively through relevant precautions.
2012, 30(1): 73-74.
doi: 10.3969/j.issn.1006-0111.2012.01.023
Abstract:
Objective To reasonably control the drug inventory, and to improve the efficiency of drug inventory management through investigating the scientific management mode of drug stock. Method The drug classification management methods were used to decrease the drug inventory, reduce the occupation of funds, and lessen the drug stock risk. Results and Conclusion The drug inventory management mode is an effective and scientific method in reducing the drug inventory cost and increasing the inventory turnover ratio.
Objective To reasonably control the drug inventory, and to improve the efficiency of drug inventory management through investigating the scientific management mode of drug stock. Method The drug classification management methods were used to decrease the drug inventory, reduce the occupation of funds, and lessen the drug stock risk. Results and Conclusion The drug inventory management mode is an effective and scientific method in reducing the drug inventory cost and increasing the inventory turnover ratio.
2012, 30(1): 75-77.
doi: 10.3969/j.issn.1006-0111.2012.01.024
Abstract:
Objective To evaluate the value of PBL teaching method in the teaching of pharmaceutical theory. Methods The subjects of the research were the scores of 4-year undergraduates of pharmacy from Grade 2001 in Hubei medical college. Results 88.6% of the students accepted teaching method of PBL, and the results of theoretical examination showad that PBL group was higher than that of the control group on the examining of general ability (P<0.05).However, there were no significant differences found between PBL group and the control group (P>0.05) in examining of knowledge. Conclusion To combine the PBL teaching with the traditionary teaching organically under present teaching condition would greatly stimulate the motivation, improve the grasp of basic knowledge and enhance the ability of knowledge application among students.
Objective To evaluate the value of PBL teaching method in the teaching of pharmaceutical theory. Methods The subjects of the research were the scores of 4-year undergraduates of pharmacy from Grade 2001 in Hubei medical college. Results 88.6% of the students accepted teaching method of PBL, and the results of theoretical examination showad that PBL group was higher than that of the control group on the examining of general ability (P<0.05).However, there were no significant differences found between PBL group and the control group (P>0.05) in examining of knowledge. Conclusion To combine the PBL teaching with the traditionary teaching organically under present teaching condition would greatly stimulate the motivation, improve the grasp of basic knowledge and enhance the ability of knowledge application among students.
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