YIN Cha, WU Ting-ting, ZHU Dong-liang, LIU Zhi-rui, CHAI Yi-feng. Determination of four major steroid glycosides in Anemarrhena asphodeloides by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 433-436. doi: 10.3969/j.issn.1006-0111.2012.06.010
| Citation:
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YIN Cha, WU Ting-ting, ZHU Dong-liang, LIU Zhi-rui, CHAI Yi-feng. Determination of four major steroid glycosides in Anemarrhena asphodeloides by HPLC-ELSD[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(6): 433-436. doi: 10.3969/j.issn.1006-0111.2012.06.010
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Determination of four major steroid glycosides in Anemarrhena asphodeloides by HPLC-ELSD
- 1.
Publishing house, Second Military Medical University, Shanghai 200433, China
- 2.
Department of Pharmacy, the 263rd Hospital of PLA, Beijing 101149, China
- 3.
Department of Pharmaceutical Analysis, School of Pharmacy, Second Military Medical University, Shanghai 200433, China
- Received Date: 2012-05-18
- Rev Recd Date:
2012-06-26
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Abstract
Objective To develop a new high performance liquid chromatography (HPLC) coupled with Evaporative Light Scattering Detector (ELSD) method for simultaneous determination of four major steroid glycosides (Timosaponin B, Timosaponin E1, Timosaponin BⅡ and Timosaponin AⅢ). Methods HPLC analysis was performed on an Agilent Zorbax SB-C18 column (4.6 mm×250 mm, 5 μm) with a mobile phase of water (adjust to pH 3.3 by acetic acid) (A) and acetonitrile (B). The gradient elution program was as follow:0~8 min, 12%~23% B;8~25 min, 23% B;25~35 min, 23%~45% B;35~40 min, 45%~95% B. The temperature of drift tube was 55℃ and the nebulizer nitrogen flow rate was 4.0 Bar. Results The linearity was obtained over 42.10~252.6 μg/ml (r=0.999 3) for Timosaponin B, 48.80~292.8 μg/ml (r=0.999 1) for Timosaponin E1, 192.2~1153 μg/ml (r=0.999 7) for Timosaponin BⅡ and 8.512~85.12 μg/ml (r=0.998 5) for Timosaponin AⅢ. The RSDs of precision and stability of the samples were both less than 1% in 48 hours. The average recovery was between 96.04%~102.8%. Conclusions The present method, with satisfactory efficacy, was simple which could simultaneously determine multiple steroid glycosides in Anemarrhena asphodeloides Bge. from different areas.
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