Study on synthesis and cyto-toxicity of polyethylene glycol-b-polylysine as potencial miRNA carrier

REN Haifeng; ZHAO Yingkui; YANG Feng; YU Yuan; MA Zhiqiang

doi:10.3969/j.issn.1006-0111.2014.05.013

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Objective To synthesize miRNA carrier PEG-b-PLL and to testify the stability, encapsulation efficiency and cytotoxicity of its complexes. Methods H¹NMR was used to determine the degree of polymerization of PLL, 4% agarose gel electrophoresis was used to determine entrapment of the polyer to the miRNA; then dynamic light scattering(DLS) was used to measure the hydrodynamic parameter such as size, polydispersity index(PDI) and zeta potential of the polyplexes. The entrapment efficiency was determined by ultraviolet-visible spectrophotometer, and finally, the cyto-toxicity of PEG-b-PLL was evaluated by CKK-8 kit with K562 cell lines. Results The characteristics indicated polyplexes prepared by PEG-b-PLL and miRNA fulfill the demand of being the gene carrier of miRNA because of low cyto-toxicity, high encapsulation efficiency and stability. Conclusion The miRNA carrier PEG-b-PLL had good character and low cyto-toxicity. It showed considerable potential as an efficient miRNA carrier.

Study on synthesis and cyto-toxicity of polyethylene glycol-b-polylysine as potencial miRNA carrier

School of Pharmacy, Second Military Medical University, Shanghai 200433, China

Received Date: 2014-02-21

Rev Recd Date: 2014-06-28

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Abstract: Objective To synthesize miRNA carrier PEG-b-PLL and to testify the stability, encapsulation efficiency and cytotoxicity of its complexes. Methods H¹NMR was used to determine the degree of polymerization of PLL, 4% agarose gel electrophoresis was used to determine entrapment of the polyer to the miRNA; then dynamic light scattering(DLS) was used to measure the hydrodynamic parameter such as size, polydispersity index(PDI) and zeta potential of the polyplexes. The entrapment efficiency was determined by ultraviolet-visible spectrophotometer, and finally, the cyto-toxicity of PEG-b-PLL was evaluated by CKK-8 kit with K562 cell lines. Results The characteristics indicated polyplexes prepared by PEG-b-PLL and miRNA fulfill the demand of being the gene carrier of miRNA because of low cyto-toxicity, high encapsulation efficiency and stability. Conclusion The miRNA carrier PEG-b-PLL had good character and low cyto-toxicity. It showed considerable potential as an efficient miRNA carrier.

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Study on synthesis and cyto-toxicity of polyethylene glycol-b-polylysine as potencial miRNA carrier

doi: 10.3969/j.issn.1006-0111.2014.05.013

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通讯作者: 陈斌, [email protected]

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