Determination of the polysaccharide in Verdihong paint

YU Xueying; CHANG Mingquan; CHEN Fang; WANG Gang

doi:10.3969/j.issn.1006-0111.2014.05.009

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Objective To establish the determination method of the polysaccharide in Verdihong paint. Methods The anhydrous dextrose was used as the control, 0.2% anthrone-sulfuric acid as the stain, the content of polysaccharide was determined by ultraviolet spectrophotometer at the wavelength of 620 nm. Results The polysaccharide curve was linear within a range of 1.9~34.2 μg/ml, linear equation was Y=0.033 X-0.006 7 (r=0.999 9), the average recovery was 98.6% with RSD of 1.06% (n=6). Conclusion This method was convenient, stable and coud be used to assay content of the polysaccharide in Verdihong paint.

Determination of the polysaccharide in Verdihong paint

Taihe Hospital Affiliated to Hubei University of Medicine, Shiyan 442000, China

Received Date: 2013-10-12

Rev Recd Date: 2013-12-27

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Abstract: Objective To establish the determination method of the polysaccharide in Verdihong paint. Methods The anhydrous dextrose was used as the control, 0.2% anthrone-sulfuric acid as the stain, the content of polysaccharide was determined by ultraviolet spectrophotometer at the wavelength of 620 nm. Results The polysaccharide curve was linear within a range of 1.9~34.2 μg/ml, linear equation was Y=0.033 X-0.006 7 (r=0.999 9), the average recovery was 98.6% with RSD of 1.06% (n=6). Conclusion This method was convenient, stable and coud be used to assay content of the polysaccharide in Verdihong paint.

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Reference (8)

[1]	常明泉,余学英,杜士明,等.维地红涂剂的制备与质量控制[J].儿科药学杂志,2012,18(11):37-39.
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[3]	陈芳,钟明哲,常明泉,等.凡胶软膏的体外释放度考察[J].医药导报,2012,31(12):1610-1612.
[4]	陈芳,常明泉,杜士明,等.不同浓度蒽酮-硫酸试液对白及溶胶含量的影响[J].中国药师,2012,15(11):1604-1606.
[5]	王爱民,王永林,郑林,等.白及药材中多糖的含量测定[J].中国中药杂志,2009,34(22):2963-2965.
[6]	张庆红,马梅芳.硫酸-蒽酮法测定天冬中多糖的含量[J].中国现代中药,2008,10(8):18-22.
[7]	李云霞,戴卫红,张彬,等.蒽酮-硫酸法测定参麦软胶囊中多糖的含量[J].解放军药学学报,2011,27(6):511-512.
[8]	马国琴,王引权,赵勇.蒽酮-硫酸比色法测定党参中可溶性糖含量的研究[J].甘肃中医学院学报,2009,26(6):46-48.

[1]	常明泉,余学英,杜士明,等.维地红涂剂的制备与质量控制[J].儿科药学杂志,2012,18(11):37-39.
[2]	国家药典委员会。中华人民共和国药典2010年版二部[S].北京:中国医药科技出版社,2010:379-380.
[3]	陈芳,钟明哲,常明泉,等.凡胶软膏的体外释放度考察[J].医药导报,2012,31(12):1610-1612.
[4]	陈芳,常明泉,杜士明,等.不同浓度蒽酮-硫酸试液对白及溶胶含量的影响[J].中国药师,2012,15(11):1604-1606.
[5]	王爱民,王永林,郑林,等.白及药材中多糖的含量测定[J].中国中药杂志,2009,34(22):2963-2965.
[6]	张庆红,马梅芳.硫酸-蒽酮法测定天冬中多糖的含量[J].中国现代中药,2008,10(8):18-22.
[7]	李云霞,戴卫红,张彬,等.蒽酮-硫酸法测定参麦软胶囊中多糖的含量[J].解放军药学学报,2011,27(6):511-512.
[8]	马国琴,王引权,赵勇.蒽酮-硫酸比色法测定党参中可溶性糖含量的研究[J].甘肃中医学院学报,2009,26(6):46-48.

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Determination of the polysaccharide in Verdihong paint

doi: 10.3969/j.issn.1006-0111.2014.05.009

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通讯作者: 陈斌, [email protected]

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