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Volume 30 Issue 5
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Article Navigation >  Journal of Pharmaceutical Practice and Service  > 2012  >  30(5): 378-379,389

ZHANG Hong-wu, REN Heng-chun, DING Gang, LI Rong-tao, WEI Jian-he, ZOU Zhong-mei. Determination of two flavones in Polygonum hydropiper Linn[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(5): 378-379,389. doi: 10.3969/j.issn.1006-0111.2012.05.017
Citation: ZHANG Hong-wu, REN Heng-chun, DING Gang, LI Rong-tao, WEI Jian-he, ZOU Zhong-mei. Determination of two flavones in Polygonum hydropiper Linn[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(5): 378-379,389. doi: 10.3969/j.issn.1006-0111.2012.05.017
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Determination of two flavones in Polygonum hydropiper Linn

doi: 10.3969/j.issn.1006-0111.2012.05.017
  • 1.

    Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100193, China

  • 2.

    Hainan Branch Institute of Medicinal Plant, Chinese Academy of Medical Science and Peking Union Medical College, Wanning 571533, China

  • Received Date: 2011-11-30
  • Rev Recd Date: 2012-05-17
  • To established the HPLC method for simultaneously determination of quercitin and quercitrin in Polygonum hydropiper Linn. Methods A VertiSep GES C18 (250 mm ×4.6 mm,5.0 μm)column was adopted with a mobile phase of acetonitrile-0.1% formic acid water(19:81) at the flow rate of 1 ml/min and the column temperature was set at 30℃.The detection wavelength was 350 nm. Results The linear range of quercitin and quercitrin was 4.827~80.455 μg／ml(r=0.999 9, n=5) and 2.934~48.900 μg/ml (r=0.999 7, n=5), respectively, and the average recovery(n=9) was 97.98%,98.29%, RSD was 1.91%,1.84% respectively. Conclusions The method was accurate, simple, reproducible, and could be used to determine the contents of quercitin and quercitrin in Polygonum hydropiper Linn.
  • [1] 全国中草药汇编编写组. 全国中草药汇编[M]. 北京:人民卫生出版社, 1986:896.
    [2] Yagi A, Uemura T, Okamura N, et al. Antioxidative sulphated flavonoids in leaves of Polygonum hydropiper[J]. Phytochemistry, 1994, 35 (4):885.
    [3] Haraguchi H, Hashimoto K, Yagi A. Antioxidative substances in leaves of Polygonum hydropiper[J]. J Agric Food Chem, 1992, 40 (8):1349.
    [4] Haraguchi H, Ohmi I, Sakai S, et al. Effect of Polygonum hydropiper flavonoids on lens aldose reductase and related enzymes[J]. J Nat Prod, 1996, 59 (4):443.
    [5] 巩忠福,杨国林,严作廷,等. 蓼属植物的化学成分与药理学活性研究进展[J]. 中草药,2002,33(1):82.
    [6] 罗杰,陆霞. HPLC法测定水辣蓼中芦丁的含量[J]. 中草药,2004,35(3):285.
    [7] 国家中医药管理局《中华本草》编委会.中华本草第6卷[M]. 上海:上海科学技术出版社,1999:662
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Determination of two flavones in Polygonum hydropiper Linn

doi: 10.3969/j.issn.1006-0111.2012.05.017
  • 1. Institute of Medicinal Plant Development, Chinese Academy of Medical Science and Peking Union Medical College, Beijing 100193, China
  • 2. Hainan Branch Institute of Medicinal Plant, Chinese Academy of Medical Science and Peking Union Medical College, Wanning 571533, China
  • Received Date: 2011-11-30
  • Rev Recd Date: 2012-05-17

Abstract: To established the HPLC method for simultaneously determination of quercitin and quercitrin in Polygonum hydropiper Linn. Methods A VertiSep GES C18 (250 mm ×4.6 mm,5.0 μm)column was adopted with a mobile phase of acetonitrile-0.1% formic acid water(19:81) at the flow rate of 1 ml/min and the column temperature was set at 30℃.The detection wavelength was 350 nm. Results The linear range of quercitin and quercitrin was 4.827~80.455 μg／ml(r=0.999 9, n=5) and 2.934~48.900 μg/ml (r=0.999 7, n=5), respectively, and the average recovery(n=9) was 97.98%,98.29%, RSD was 1.91%,1.84% respectively. Conclusions The method was accurate, simple, reproducible, and could be used to determine the contents of quercitin and quercitrin in Polygonum hydropiper Linn.

ZHANG Hong-wu, REN Heng-chun, DING Gang, LI Rong-tao, WEI Jian-he, ZOU Zhong-mei. Determination of two flavones in Polygonum hydropiper Linn[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(5): 378-379,389. doi: 10.3969/j.issn.1006-0111.2012.05.017
Citation: ZHANG Hong-wu, REN Heng-chun, DING Gang, LI Rong-tao, WEI Jian-he, ZOU Zhong-mei. Determination of two flavones in Polygonum hydropiper Linn[J]. Journal of Pharmaceutical Practice and Service, 2012, 30(5): 378-379,389. doi: 10.3969/j.issn.1006-0111.2012.05.017
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