酸性染料比色法测定复方颠茄合剂中莨菪碱含量
Determination of content of hyoscyamine in compound belladonna mixture by acid dye colorimetry
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摘要: 目的： 研究复方颠茄合剂中莨菪碱的含量测定方法。 方法： 采用酸性染料比色法在pH6．6磷酸盐缓冲液条件下测定莨菪碱的含量,检测波长(589±1)nm。 结果： 莨菪碱在1．2～7．2μg/mL浓度范围内呈线性,r=0．9991,平均回收率为100．02%,(RSD=0．69%,n=4)。 结论： 该法准确、方便、灵敏,可供该制剂的含量测定。该法关键在于调准pH,以免处方中其它组分干扰测定结果。Abstract: Objective: To determine hyoscyamine in compound belladonna mixture. Methods: Acid dye colorimetry was used in pH 6.6 with the detection wavelength at(589±1)nm. Results: The linear ranges of the standard curve was 1.2～7.2μg/mL(r= 0.9991).The average recovery of the assay was 100.02%(RSD=0.69%,n=4). Conclusions: This method is accurate,convenient and sensitive for the quality control of hyoscyamine in compound belladonna mixture.The key of this method is exactly control of pH in order to avoid the disturbance.
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Key words:
- acid dye colorimetry /
- compound belladonna mixture /
- hyoscyamine /
- content
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