Analysis on chemical constituents of ershen pill by HPLC-TOF/MS

JI Song-gang; WU Hai-tang; MIAO Hua-yan; WU Si; LI Xiang

Message Board

Respected readers, authors and reviewers, you can add comments to this page on any questions about the contribution, review, editing and publication of this journal. We will give you an answer as soon as possible. Thank you for your support!

Name

E-mail

Phone

Title

Content

Verification Code

Objective To analyze chemical constituents of ershen pill by rapid-resolution(high performance) liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods A shelloseido column(3.0 mm×100 mm,3 μm) was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.6 ml/min. TOF/MS was applied for qualitative analysis under positive ion mode. Results Under LC/MS condition, 21 major constituents in ershen pill were identified by time of flight mass spectrometry, among which, 15 constituents were from Psoralea corylifolia L,and 6 constituents were from Myristica fragrans Houtt. Conclusion A simple and reliable method using HPLC-TOF/MS was established to identify the chemical constituents of ershen pill.

Analysis on chemical constituents of ershen pill by HPLC-TOF/MS

1. Department of Pharmacy, No.401 Hospital of PLA, Qingdao 266071, China

2. School of Pharmacy, Second Military Medical University, Shanghai 200433, China

3. No.62370 PLA troop, Beijing 100071, China

4. No. 62380 PLA troop, Beijing 100071, China

Received Date: 2011-01-18

Rev Recd Date: 2011-10-21

Key words:

Abstract: Objective To analyze chemical constituents of ershen pill by rapid-resolution(high performance) liquid chromatography-time of flight mass spectrometry (HPLC-TOF/MS). Methods A shelloseido column(3.0 mm×100 mm,3 μm) was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.6 ml/min. TOF/MS was applied for qualitative analysis under positive ion mode. Results Under LC/MS condition, 21 major constituents in ershen pill were identified by time of flight mass spectrometry, among which, 15 constituents were from Psoralea corylifolia L,and 6 constituents were from Myristica fragrans Houtt. Conclusion A simple and reliable method using HPLC-TOF/MS was established to identify the chemical constituents of ershen pill.

HTML

Reference (7)

[1]	王缙,付庆会. 宋明期间二神丸和五味子散两方的发展演变[J]. 四川中医,2010,28:115.
[2]	唐昌娟, 施贝, 修彦凤,等. 二神汤中补骨脂素_异补骨脂素的药动学研究[J]. 上海中医药杂志,2011,45:72.
[3]	李冀, 邹大威, 杜雅薇,等. 二神丸与四神丸对脾虚泄泻大鼠分泌型IgA白介素-2含量影响的配伍比较研究[J]. 中华中医药学刊,2007,25:2437.
[4]	李冀, 邹大威, 杜雅薇,等. 二神丸与四神丸对脾虚泄泻作用的配伍比较研究[J]. 辽宁中医杂志,2007,34:728.
[5]	Zhan ZJ, Fan CQ, Ding J, et al. Novel diterpenoids with potent inhibitory activity against endothelium cell HMEC and cytotoxic activities from a well-known TCM plant Daphne genkwa[J]. Bioorg Med Chem,2005,13:645.
[6]	Zhu Z, Zhang H, Zhao L, et al. Rapid separation and identification of phenolic and diterpenoid constituents from Radix Salvia miltiorrhizae by high-performance liquid chromatography diode-array detection, electrospray ionization time-of-flight mass spectrometry and electrospray ionization quadrupole ion trap mass spectrometry[J]. Rapid Commun Mass Spectrom,2007,21:1855.
[7]	Zhao L, Lou ZY, Zhu ZY, et al. Characterization of constituents in Stellera chamaejasme L. by rapid-resolution liquid chromatography-diode array detection and electrospray ionization time-of-flight mass spectrometry[J]. Biomed Chromatogr,2008,22:64.

Message Board

Analysis on chemical constituents of ershen pill by HPLC-TOF/MS

Abstract

References

Proportional views

通讯作者: 陈斌, [email protected]

Article Metrics

Related

Proportional views