Simultaneous determination of 5 B-vitamins in compound trivitamin and calcium pantothenate syrup by UHILIC-MS/MS

REN Qiangqiang; ZHAO Youqin; XU Wen; HUANG Mingqing; YE Shuiguo; SHI Zhenxiang

doi:10.3969/j.issn.1006-0111.2018.06.008

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Objective To establish a ultra hydrophilic interaction chromatography-tandem mass spectrometry (UHILIC-MS/MS) for the simultaneous determination of 5 B-vitamins including thiamine, riboflavin, pyridoxine, nicotinamide and calcium pantothenate in Compound Trivitamin and Calcium Pantothenate Syrup. Methods The chromatographic separation was performed on a Waters ACQUITY UHILIC BEH Amide (2.1 mm×100 mm,1.7 μm) column with the mobile phase consisting of 10 mmol/L ammonium formate water and 90% acetonitrile both containing 0.5% formic acid water in a gradient elution at a flow rate of 0.30 ml/min. An electrospray positive ion multiple reaction monitoring(MRM) was used as a detector. Results Five B-vitamins in the sample achieved excellent linearity calibration curves over the concentration range tested (r>0.998 4) within 5 min. The overall average recovery rates were in the range of 93.27%-100.39% with RSD of 1.41%-4.96%. The contents of thiamine, riboflavin, pyridoxine, nicotinamide and calcium pantothenate in ten samples were 32.40-38.91, 7.002-8.462, 9.677-11.17, 33.64-39.58, 3.276-3.771 mg/250 g, respectively. Conclusion The UHILIC-MS/MS method established in this study could quickly achieve qualitative identification or quantitative detection of five B-vitamins components in Compound Trivitamin and Calcium Pantothenate Syrup, which provided a reliable technical detection method for the development, utilization and quality evaluation of Compound Trivitamin and Calcium Pantothenate Syrup.

Simultaneous determination of 5 B-vitamins in compound trivitamin and calcium pantothenate syrup by UHILIC-MS/MS

1. School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fujian Key Laboratory of Chinese Materia Medica, Fuzhou 350122, China

2. Sinopharm Xingsha Pharmaceuticals(Xiamen) Co., Ltd., Xiamen 361028, China

Received Date: 2018-05-11

Rev Recd Date: 2018-10-09

Key words:

Compound Trivitamin and Calcium Pantothenate Syrup /
ultra hydrophilic interaction chromatography-mass spectrometry/mass spectrometry /
thiamine /
riboflavin /
pyridoxine /
nicotinamide /
calcium pantothenate /
content determination

Abstract: Objective To establish a ultra hydrophilic interaction chromatography-tandem mass spectrometry (UHILIC-MS/MS) for the simultaneous determination of 5 B-vitamins including thiamine, riboflavin, pyridoxine, nicotinamide and calcium pantothenate in Compound Trivitamin and Calcium Pantothenate Syrup. Methods The chromatographic separation was performed on a Waters ACQUITY UHILIC BEH Amide (2.1 mm×100 mm,1.7 μm) column with the mobile phase consisting of 10 mmol/L ammonium formate water and 90% acetonitrile both containing 0.5% formic acid water in a gradient elution at a flow rate of 0.30 ml/min. An electrospray positive ion multiple reaction monitoring(MRM) was used as a detector. Results Five B-vitamins in the sample achieved excellent linearity calibration curves over the concentration range tested (r>0.998 4) within 5 min. The overall average recovery rates were in the range of 93.27%-100.39% with RSD of 1.41%-4.96%. The contents of thiamine, riboflavin, pyridoxine, nicotinamide and calcium pantothenate in ten samples were 32.40-38.91, 7.002-8.462, 9.677-11.17, 33.64-39.58, 3.276-3.771 mg/250 g, respectively. Conclusion The UHILIC-MS/MS method established in this study could quickly achieve qualitative identification or quantitative detection of five B-vitamins components in Compound Trivitamin and Calcium Pantothenate Syrup, which provided a reliable technical detection method for the development, utilization and quality evaluation of Compound Trivitamin and Calcium Pantothenate Syrup.

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Simultaneous determination of 5 B-vitamins in compound trivitamin and calcium pantothenate syrup by UHILIC-MS/MS

doi: 10.3969/j.issn.1006-0111.2018.06.008

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