TLC identification and determination of total flavonoids in Compound caulis Polygoni multiflori mixture

ZENG Qiping; CAO Yixiang; HUANG Lishan; ZHANG Rong

doi:10.3969/j.issn.1006-0111.2016.05.011

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Objective To establish the quality control standards for Compound caulis Polygoni multiflori mixture. Methods Caulis polygoni multiflori, cortex alibiziae and schisand chinensis were identified by TLC, total flavonoids were determined by UV. Results Caulis polygoni multiflori,cortex alibiziae and schisand chinensis could be detected by TLC. A better linear relationship between concentration and absorbance in the range of 9.12~27.36 μg/ml. The linear regression equation was A=0.034 55 C-0.110 34 (r=0.999 2), The average recovery of emodin was 101.0%(RSD=1.58%). Conclusion The method is simple, quick, accurate and sensitive, which could be used as a quantitative analysis method for this preparation.

TLC identification and determination of total flavonoids in Compound caulis Polygoni multiflori mixture

The Pharmacy Department, No. 175 Hospital of PLA, Zhangzhou 363000, China

Received Date: 2015-02-05

Rev Recd Date: 2015-08-02

Key words:

Compound caulis Polygoni multiflori mixture /
TLC /
total flavonoids /
ultraviolet spectrophometry /
content

Abstract: Objective To establish the quality control standards for Compound caulis Polygoni multiflori mixture. Methods Caulis polygoni multiflori, cortex alibiziae and schisand chinensis were identified by TLC, total flavonoids were determined by UV. Results Caulis polygoni multiflori,cortex alibiziae and schisand chinensis could be detected by TLC. A better linear relationship between concentration and absorbance in the range of 9.12~27.36 μg/ml. The linear regression equation was A=0.034 55 C-0.110 34 (r=0.999 2), The average recovery of emodin was 101.0%(RSD=1.58%). Conclusion The method is simple, quick, accurate and sensitive, which could be used as a quantitative analysis method for this preparation.

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TLC identification and determination of total flavonoids in Compound caulis Polygoni multiflori mixture

doi: 10.3969/j.issn.1006-0111.2016.05.011

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通讯作者: 陈斌, [email protected]

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