Effect of litchi saponin and litchi flavones on insulin resistance in HepG2 cells

XIAO Zhijun; GUO Jiewen; XU Feng

doi:10.3969/j.issn.1006-0111.2015.04.007

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Objective To study the effect of litchi saponin and flavones on glycometabolism in insulin resistance model of HepG2 cells. Methods Using high insulin in HepG2 cells to establish a cell model of insulin resistance. Cell model was treated by litchi saponin, litchi flavones and rosiglitazone, respectively. Glucose concentration of cell culture supernatant was detected by glucose oxidase method. Results In the concentration of 10^-6 mol/L insulin for 24 hours, HepG2 reached the highest level of resistant to insulin, which means a successful insulin resistance cell model was established. However, no significant decrease in glucose concentration of cell culture supernatant was observed with litchi saponin and flavones. Conclusion This study suggests that litchi saponin and flavones have no effect to improve insulin resistance.

Effect of litchi saponin and litchi flavones on insulin resistance in HepG2 cells

1. Department of Pharmacy, Fengxian Central Hospital, Shanghai 201400, China

2. Guangzhou Hospital of Traditional Chinese Medicine, Guangzhou 510130, China

Received Date: 2015-04-22

Rev Recd Date: 2015-06-06

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Abstract: Objective To study the effect of litchi saponin and flavones on glycometabolism in insulin resistance model of HepG2 cells. Methods Using high insulin in HepG2 cells to establish a cell model of insulin resistance. Cell model was treated by litchi saponin, litchi flavones and rosiglitazone, respectively. Glucose concentration of cell culture supernatant was detected by glucose oxidase method. Results In the concentration of 10^-6 mol/L insulin for 24 hours, HepG2 reached the highest level of resistant to insulin, which means a successful insulin resistance cell model was established. However, no significant decrease in glucose concentration of cell culture supernatant was observed with litchi saponin and flavones. Conclusion This study suggests that litchi saponin and flavones have no effect to improve insulin resistance.

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Effect of litchi saponin and litchi flavones on insulin resistance in HepG2 cells

doi: 10.3969/j.issn.1006-0111.2015.04.007

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