2016 Vol. 34, No. 1
Display Method:
2016, 34(1): 1-4,55.
doi: 10.3969/j.issn.1006-0111.2016.01.001
Abstract:
Breast cancer has become the main malignant tumors which pose a serious threat to women's health.Selective estrogen receptor modulators, which served as the effective drug treatment,have been attracting more attention. Present research progress on the selective estrogen receptor modulators was summarized in this paper.
Breast cancer has become the main malignant tumors which pose a serious threat to women's health.Selective estrogen receptor modulators, which served as the effective drug treatment,have been attracting more attention. Present research progress on the selective estrogen receptor modulators was summarized in this paper.
2016, 34(1): 5-7,15.
doi: 10.3969/j.issn.1006-0111.2016.01.002
Abstract:
Glycosylation is the key step of the synthesis of GM3, its reaction conditions are very harsh, the stereoselectivities are usually poor, and the configuration of anomeric carbon is difficult to control. Whether α glycosidic bond can be constructed efficiently in sialylation reactions is an important criteria used to evaluate the reaction quality. Studies of GM3 and derivatives methods generally relates to following areas: the choice of the donor compounds and receptor compounds, the control of stereoselectivity, and the development of some new glycosidic reaction catalyst. In recent years, important progress has been made in this research area. Now, we predominately make a summary and review on the progress of methods for the synthesis of GM3 and derivatives.
Glycosylation is the key step of the synthesis of GM3, its reaction conditions are very harsh, the stereoselectivities are usually poor, and the configuration of anomeric carbon is difficult to control. Whether α glycosidic bond can be constructed efficiently in sialylation reactions is an important criteria used to evaluate the reaction quality. Studies of GM3 and derivatives methods generally relates to following areas: the choice of the donor compounds and receptor compounds, the control of stereoselectivity, and the development of some new glycosidic reaction catalyst. In recent years, important progress has been made in this research area. Now, we predominately make a summary and review on the progress of methods for the synthesis of GM3 and derivatives.
2016, 34(1): 8-11,47.
doi: 10.3969/j.issn.1006-0111.2016.01.003
Abstract:
The occurrence of the clinical manifestations of myocardial ischemia shows clear circadian rhythmicity, and they are unevenly distributed during the 24 h with higher morbidity during the initial hours of the daily activity span and in the late afternoon or early evening. Such temporal patterns result from circadian rhythms in pathophysiological mechanisms plus cyclic environmental stressors that trigger these clinical events. β-receptor antagonist medications, oral nitrate, and calcium channel blocker have been shown to be influenced by the circadian time of their administration. Here we briefly review the characteristics of circadian rhythmicity in MI, the pathophysiological mechanisms as well as the current chronotherapy,and then discuss the future treatment strategies.
The occurrence of the clinical manifestations of myocardial ischemia shows clear circadian rhythmicity, and they are unevenly distributed during the 24 h with higher morbidity during the initial hours of the daily activity span and in the late afternoon or early evening. Such temporal patterns result from circadian rhythms in pathophysiological mechanisms plus cyclic environmental stressors that trigger these clinical events. β-receptor antagonist medications, oral nitrate, and calcium channel blocker have been shown to be influenced by the circadian time of their administration. Here we briefly review the characteristics of circadian rhythmicity in MI, the pathophysiological mechanisms as well as the current chronotherapy,and then discuss the future treatment strategies.
2016, 34(1): 12-15.
doi: 10.3969/j.issn.1006-0111.2016.01.004
Abstract:
Inflammasome is the body of protein complex which is able to identify different stimulation signals and can induce immune and inflammatory responses when it is activated. NLRP3 inflammasome has been extensively studied in the central nervous system. Microglia, perivascular macrophages and meningeal macrophages express inflammasomes. The occurrence and development of acute brain infection, aseptic acute brain injury, Parkinson disease and Alzheimer disease are closely related to inflammasomes. Based on mechanisms exploration of the inflammasome's role in the central nervous system disorders, its target drug research and development will be greatly addressed in the future.
Inflammasome is the body of protein complex which is able to identify different stimulation signals and can induce immune and inflammatory responses when it is activated. NLRP3 inflammasome has been extensively studied in the central nervous system. Microglia, perivascular macrophages and meningeal macrophages express inflammasomes. The occurrence and development of acute brain infection, aseptic acute brain injury, Parkinson disease and Alzheimer disease are closely related to inflammasomes. Based on mechanisms exploration of the inflammasome's role in the central nervous system disorders, its target drug research and development will be greatly addressed in the future.
2016, 34(1): 16-18.
doi: 10.3969/j.issn.1006-0111.2016.01.005
Abstract:
Objective This article reviewed the moisture-proof strategies of Chinese meteria medica preparation to provide a basis for quality control of traditional Chinese medicine. Methods Review, analyze and summarize through referring to relevant literatures in recent years. Results The hygroscopicity of Chinese meteria medica preparation can be influnced by extractive techniques, auxiliary material and application of film coating. Conclution Study on techniques of Chinese meteria medica preparation needs further in-depth investigation.
Objective This article reviewed the moisture-proof strategies of Chinese meteria medica preparation to provide a basis for quality control of traditional Chinese medicine. Methods Review, analyze and summarize through referring to relevant literatures in recent years. Results The hygroscopicity of Chinese meteria medica preparation can be influnced by extractive techniques, auxiliary material and application of film coating. Conclution Study on techniques of Chinese meteria medica preparation needs further in-depth investigation.
2016, 34(1): 19-23,40.
doi: 10.3969/j.issn.1006-0111.2016.01.006
Abstract:
Objective To study and optimize the preparation condition of pVAX1-wapA-loaded nanoparticles and determine the transfection efficiency. Methods The related effects of the crucial factors for the formation of nanoparticles: concentration of chitosan and TPP, pH value, N/P ratio were studied by single-factor experiment, with nanoparticles size and zeta potential as index. Cell transfection test was carried out to indicate that enhancement of cell transfection efficiency of nano-carrier. Results Nanoparticles loaded DNA vaccine were nearly spherical shape with uniform particle size chitosan nanoparticle(CS),(219.2±18.2) nm;quaternary ammonium chitosan nanoparticles(CSTM),(222.5±15.6) nm. Zeta potential of CS and CSTM was (24.7±3.5) mV, (19.6±1.2) mV and encapsulation efficiency was 91.24%, 87.66%,respectively.CSTM nanoparticle could enhance cellular uptake of pVAX1-wapA obviously. Conclusion CSTM nanoparticle was proved to be an efficient DNA vaccine delivery vector.
Objective To study and optimize the preparation condition of pVAX1-wapA-loaded nanoparticles and determine the transfection efficiency. Methods The related effects of the crucial factors for the formation of nanoparticles: concentration of chitosan and TPP, pH value, N/P ratio were studied by single-factor experiment, with nanoparticles size and zeta potential as index. Cell transfection test was carried out to indicate that enhancement of cell transfection efficiency of nano-carrier. Results Nanoparticles loaded DNA vaccine were nearly spherical shape with uniform particle size chitosan nanoparticle(CS),(219.2±18.2) nm;quaternary ammonium chitosan nanoparticles(CSTM),(222.5±15.6) nm. Zeta potential of CS and CSTM was (24.7±3.5) mV, (19.6±1.2) mV and encapsulation efficiency was 91.24%, 87.66%,respectively.CSTM nanoparticle could enhance cellular uptake of pVAX1-wapA obviously. Conclusion CSTM nanoparticle was proved to be an efficient DNA vaccine delivery vector.
2016, 34(1): 24-27,51.
doi: 10.3969/j.issn.1006-0111.2016.01.007
Abstract:
Objective To study the chemical constituents of Ixeris sonchifolia. Methods Monomeric compounds were isolated by chromatography on silica gel column, Sephadex LH-20, ODS chromatography column and HPLC.Thestructure of the compound wasconfirmed on the basis of physio-chemical constants and spectroscopic analysis. 2,2-diphenyl-1-pikrylhydrazyl (DPPH) and 3-ethylbenzthiazoline-6-sulfonic acid(ABTS)assay were usedto evaluate the antioxidant activities of isolated compounds. Results Five compounds were isolated from 70% ethanol-water extraction and their structures were identified as (+)-pinoresinol-4-O-β-D-glucopyranoside( 1 ), glochidioboside( 2 ), 3,4,5-trimethoxyphenyl-1-O-β-D-glucopy-ranoside( 3 ), ononin( 4 ),daidzein( 5 ), luteolin ( 6 ), and apigenin ( 7 ). Meanwhile, antioxidant activities of all the isolates were evaluated by DPPHand ABTS. Conclusion Compounds 1-5 were isolated from this genus for the first time. The active results showed that 1, 2, 4-7 exhibited potent antioxidant activity.
Objective To study the chemical constituents of Ixeris sonchifolia. Methods Monomeric compounds were isolated by chromatography on silica gel column, Sephadex LH-20, ODS chromatography column and HPLC.Thestructure of the compound wasconfirmed on the basis of physio-chemical constants and spectroscopic analysis. 2,2-diphenyl-1-pikrylhydrazyl (DPPH) and 3-ethylbenzthiazoline-6-sulfonic acid(ABTS)assay were usedto evaluate the antioxidant activities of isolated compounds. Results Five compounds were isolated from 70% ethanol-water extraction and their structures were identified as (+)-pinoresinol-4-O-β-D-glucopyranoside( 1 ), glochidioboside( 2 ), 3,4,5-trimethoxyphenyl-1-O-β-D-glucopy-ranoside( 3 ), ononin( 4 ),daidzein( 5 ), luteolin ( 6 ), and apigenin ( 7 ). Meanwhile, antioxidant activities of all the isolates were evaluated by DPPHand ABTS. Conclusion Compounds 1-5 were isolated from this genus for the first time. The active results showed that 1, 2, 4-7 exhibited potent antioxidant activity.
2016, 34(1): 28-31,61.
doi: 10.3969/j.issn.1006-0111.2016.01.008
Abstract:
Objective To study the effect of coptis decoction on rats with ulcerative colitis. Methods Rat ulcerative colitis model was induced by 1,2-dimethylhydrazine(DMH)(40 mg/kg s.c.)+3% dextran sodium sulfate solution(DSS)(freely drinking). The drugs were given orally once a day for 7 day per week. After 5 weeks, the body weight, the number of colon ACFs and the number of colon cancer in rats, as well as the morphological changes of rat colon tissues were evaluated. Results Coptis decoction significantly improved the dyscrasia induced by DMH+DSS, the both of body weight and general condition were better than those of control group(P<0.05,P<0.01). Coptis decoction also significantly inhibited ACF in the rats treated by DMH+DSS for 10 weeks(P<0.01). The model group showed typical inflamed mucosa, a nearly complete subversion of the normal surface architecture. Compared with the model group, coptis decoction in each dosage group could improve rat atypical hyperplasia, mucosal hyperemia, edema and cells in the crowded. Transmission electron microscope showed that, in epithelial nuclei chromatin aggregation in the model group, the cell surface microvilli arranged disorder, fault phenomenon. Compared with model group, the coptis decoction had improved significantly. Conclusion Coptis decoction had significant therapeutic effect on rat ulcerative colitis.
Objective To study the effect of coptis decoction on rats with ulcerative colitis. Methods Rat ulcerative colitis model was induced by 1,2-dimethylhydrazine(DMH)(40 mg/kg s.c.)+3% dextran sodium sulfate solution(DSS)(freely drinking). The drugs were given orally once a day for 7 day per week. After 5 weeks, the body weight, the number of colon ACFs and the number of colon cancer in rats, as well as the morphological changes of rat colon tissues were evaluated. Results Coptis decoction significantly improved the dyscrasia induced by DMH+DSS, the both of body weight and general condition were better than those of control group(P<0.05,P<0.01). Coptis decoction also significantly inhibited ACF in the rats treated by DMH+DSS for 10 weeks(P<0.01). The model group showed typical inflamed mucosa, a nearly complete subversion of the normal surface architecture. Compared with the model group, coptis decoction in each dosage group could improve rat atypical hyperplasia, mucosal hyperemia, edema and cells in the crowded. Transmission electron microscope showed that, in epithelial nuclei chromatin aggregation in the model group, the cell surface microvilli arranged disorder, fault phenomenon. Compared with model group, the coptis decoction had improved significantly. Conclusion Coptis decoction had significant therapeutic effect on rat ulcerative colitis.
2016, 34(1): 32-35,65.
doi: 10.3969/j.issn.1006-0111.2016.01.009
Abstract:
Objective To investigate the hemostatic effect of Bletillastriata hemostatic sponge. Methods Tail hemorrhage model in rats was preparation. Another model was established in liver and spleen hemorrhagic model of rabbits, Beagle dog's abdominal aorta and liver were made. Hemostasis was performed with medical gauze, gelatin sponge and Bletillastriata hemostatic sponge. The hemostatic effects were evaluated by total blood loss, hemostatic time and histological observation. Observation of Bletillastriata hemostatic sponge change was completed to determine their degradation in the bodies. Results Rats tail hemorrhage was clamped, after 1 min, Bletillastriata hemostatic sponge could effectively stop the bleeding.The Bletillastriata hemostatic sponge has good hemostatic effect of rats tail hemorrhage model compared to blank group(P<0.01). Compare with the gelatin sponge group, the Bletillastriata hemostatic sponge could significantly shorten the liver and spleen bleeding time(P<0.05), decrease the volume of the spleen bleeding(P<0.05) and the liver bleeding(P<0.01), in hemorrhagic model of rabbits. Compared with the gelatin sponge group, Bletillastriata hemostatic sponge could more effectively stop the bleeding in the abdominal aorta and liver model of Beagle dogs (P<0.05). The Bletillastriata hemostatic sponge could be degraded in vivo. Histologic study revealed the Bletillastriata hemostatic sponge was no significant pathological change around the liver and spleen. Conclusion The Bletillastriata hemostatic sponge has good hemostatic effect. The hemostatic effect of Bletillastriata hemostatic sponge is better than gelatin sponge, which could be a good topical hemostatic material.
Objective To investigate the hemostatic effect of Bletillastriata hemostatic sponge. Methods Tail hemorrhage model in rats was preparation. Another model was established in liver and spleen hemorrhagic model of rabbits, Beagle dog's abdominal aorta and liver were made. Hemostasis was performed with medical gauze, gelatin sponge and Bletillastriata hemostatic sponge. The hemostatic effects were evaluated by total blood loss, hemostatic time and histological observation. Observation of Bletillastriata hemostatic sponge change was completed to determine their degradation in the bodies. Results Rats tail hemorrhage was clamped, after 1 min, Bletillastriata hemostatic sponge could effectively stop the bleeding.The Bletillastriata hemostatic sponge has good hemostatic effect of rats tail hemorrhage model compared to blank group(P<0.01). Compare with the gelatin sponge group, the Bletillastriata hemostatic sponge could significantly shorten the liver and spleen bleeding time(P<0.05), decrease the volume of the spleen bleeding(P<0.05) and the liver bleeding(P<0.01), in hemorrhagic model of rabbits. Compared with the gelatin sponge group, Bletillastriata hemostatic sponge could more effectively stop the bleeding in the abdominal aorta and liver model of Beagle dogs (P<0.05). The Bletillastriata hemostatic sponge could be degraded in vivo. Histologic study revealed the Bletillastriata hemostatic sponge was no significant pathological change around the liver and spleen. Conclusion The Bletillastriata hemostatic sponge has good hemostatic effect. The hemostatic effect of Bletillastriata hemostatic sponge is better than gelatin sponge, which could be a good topical hemostatic material.
2016, 34(1): 36-40.
doi: 10.3969/j.issn.1006-0111.2016.01.010
Abstract:
Objective To prepare gemcitabine hydrochloride thermosensitive gel injection and to stablish the determination methods of its contents. Methods Gemcitabine hydrochloride thermosensitive gel injection was prepared using PLGA-PEG-PLGA as thermosensitive viecle. The contents of gemcitabine hydrochloride were determined by HPLC. Results The formulation contained 40 mg/ml gemcitabine and 20% (wt) PLGA-PEG-PLGA with phase-transition temperature of (37±0.15) ℃, showing the best viscosity around human body temperature. Gemcitabine hydrochloride presented a good linearity in the range of 5-500 μg/ml(r=0.999 8), which had good precision and reproducibility. The recovery rate of low, middle and high concentrations of gemcitabine hydrochloride were (99.5±3.2)%, (100.4±2.4)%, (102.1±2.4)%,n=3, respectively. The average contents of gemcitabine hydrochloride in three batches of sample were (101.87±2.95)%, (99.4±2.73)%, (98.98±0.71)%, n=3, respectively. Conclusion The quality of gemcitabine hydrochloride thermosensitive gel injection with PLGA-PEG-PLGA as matrix could be controlled. It is a promising new drug for pancreatic cancer.
Objective To prepare gemcitabine hydrochloride thermosensitive gel injection and to stablish the determination methods of its contents. Methods Gemcitabine hydrochloride thermosensitive gel injection was prepared using PLGA-PEG-PLGA as thermosensitive viecle. The contents of gemcitabine hydrochloride were determined by HPLC. Results The formulation contained 40 mg/ml gemcitabine and 20% (wt) PLGA-PEG-PLGA with phase-transition temperature of (37±0.15) ℃, showing the best viscosity around human body temperature. Gemcitabine hydrochloride presented a good linearity in the range of 5-500 μg/ml(r=0.999 8), which had good precision and reproducibility. The recovery rate of low, middle and high concentrations of gemcitabine hydrochloride were (99.5±3.2)%, (100.4±2.4)%, (102.1±2.4)%,n=3, respectively. The average contents of gemcitabine hydrochloride in three batches of sample were (101.87±2.95)%, (99.4±2.73)%, (98.98±0.71)%, n=3, respectively. Conclusion The quality of gemcitabine hydrochloride thermosensitive gel injection with PLGA-PEG-PLGA as matrix could be controlled. It is a promising new drug for pancreatic cancer.
2016, 34(1): 41-43,75.
doi: 10.3969/j.issn.1006-0111.2016.01.011
Abstract:
Objective To develop a simple and sensitive HPLC-UV method to determined actarit in plasma. Methods The chromatographic separation was carried out on a Dikma C18 column with a mobile phase composed of water containing 1.2% acetic acid-methanol (50:50, V/V) at a flow rate of 1.0 ml/min and the temperature of column was 30℃. UV wavelength mode was monitored at 245 nm. Results A linear response function was established for the range of concentrations 75-4 000 ng/ml for actarit. The inter-and intra-day precision value were less than 10% for actarit and the accuracy was more than 90%. The extraction recovery was more than 88%. Conclusion This simple, sensitive, and exclusive method could be used in routine clinical practice to monitor actarit concentrations in human plasma.
Objective To develop a simple and sensitive HPLC-UV method to determined actarit in plasma. Methods The chromatographic separation was carried out on a Dikma C18 column with a mobile phase composed of water containing 1.2% acetic acid-methanol (50:50, V/V) at a flow rate of 1.0 ml/min and the temperature of column was 30℃. UV wavelength mode was monitored at 245 nm. Results A linear response function was established for the range of concentrations 75-4 000 ng/ml for actarit. The inter-and intra-day precision value were less than 10% for actarit and the accuracy was more than 90%. The extraction recovery was more than 88%. Conclusion This simple, sensitive, and exclusive method could be used in routine clinical practice to monitor actarit concentrations in human plasma.
2016, 34(1): 44-47.
doi: 10.3969/j.issn.1006-0111.2016.01.012
Abstract:
Objective To prepare hot-melt pressure sensitive adhesive (HMPSA) transdermal patches of progesterone and investigate the release characterization in vitro. Methods SIS HMPSA was used as matrix material, modified Franz diffusion cell and excised female rat skin were used as model,the concentration of progesterone was determined by HPLC. The thickness of patch and penetration enhancers was screened out by permeation rate. Results 2% IPM was used to prepare progesterone HMPSA transdermal patch, thickness was 300 μm, the cumulative permeation curve was Q=6.172 1 t-5.457 7 (r=0.998 8). The cumulative releases of the patches in 24 h was 144.17 μg/cm2. The permeation rate was (6.17±0.49) μg/(cm2·h). Conclusion The progesterone HMPSA transdermal patch had good transdermal release in vitro, which would have good clinical application prospect.
Objective To prepare hot-melt pressure sensitive adhesive (HMPSA) transdermal patches of progesterone and investigate the release characterization in vitro. Methods SIS HMPSA was used as matrix material, modified Franz diffusion cell and excised female rat skin were used as model,the concentration of progesterone was determined by HPLC. The thickness of patch and penetration enhancers was screened out by permeation rate. Results 2% IPM was used to prepare progesterone HMPSA transdermal patch, thickness was 300 μm, the cumulative permeation curve was Q=6.172 1 t-5.457 7 (r=0.998 8). The cumulative releases of the patches in 24 h was 144.17 μg/cm2. The permeation rate was (6.17±0.49) μg/(cm2·h). Conclusion The progesterone HMPSA transdermal patch had good transdermal release in vitro, which would have good clinical application prospect.
2016, 34(1): 48-51.
doi: 10.3969/j.issn.1006-0111.2016.01.013
Abstract:
Objective To establish a method for the determination of quetiapine fumarate in human serum by RP-HPLC and apply it into clinical. Methods Extracting with ethyl ether after serum-drug was alkalized, and then determined by RP-HPLC. The determination was performed on Zorbax Eclipse XDB-C18 column with mobile phase consisted of methanol-water (70:30, containing 0.5% triethylamine and 0.4% glacial acetic acid) at the flow rate of 0.6 ml/min. The detection wavelength was set at 254 nm, and the column temperature was 35 ℃. The method would be applied into analysis of clinical medication. Results Quetiapine fumarate and the impurities could be completely separated, and the linear range of quetiapine fumarate were 50-1 000 ng/ml(r=0.999 5).The recovery of the method was 98.2%-100.1% and the recovery of extracting was 75.2%-84.6%. RSD of intra-day was within 0.8%-3.7% and RSD of inter-day was within 1.4%-5.1%.The limit of quantitation for quetiapine fumarate was 2.1 ng/ml.This method had been applied into clinical pharmacy and achieved a good effects. Conclusions The method is simple, accurate, reproducible, and sensitive for determination of quetiapine fumarate in human serum. It has important significance on instructing the rational use of clinical medicine and discovering the unreasonable drug combination.
Objective To establish a method for the determination of quetiapine fumarate in human serum by RP-HPLC and apply it into clinical. Methods Extracting with ethyl ether after serum-drug was alkalized, and then determined by RP-HPLC. The determination was performed on Zorbax Eclipse XDB-C18 column with mobile phase consisted of methanol-water (70:30, containing 0.5% triethylamine and 0.4% glacial acetic acid) at the flow rate of 0.6 ml/min. The detection wavelength was set at 254 nm, and the column temperature was 35 ℃. The method would be applied into analysis of clinical medication. Results Quetiapine fumarate and the impurities could be completely separated, and the linear range of quetiapine fumarate were 50-1 000 ng/ml(r=0.999 5).The recovery of the method was 98.2%-100.1% and the recovery of extracting was 75.2%-84.6%. RSD of intra-day was within 0.8%-3.7% and RSD of inter-day was within 1.4%-5.1%.The limit of quantitation for quetiapine fumarate was 2.1 ng/ml.This method had been applied into clinical pharmacy and achieved a good effects. Conclusions The method is simple, accurate, reproducible, and sensitive for determination of quetiapine fumarate in human serum. It has important significance on instructing the rational use of clinical medicine and discovering the unreasonable drug combination.
2016, 34(1): 52-55.
doi: 10.3969/j.issn.1006-0111.2016.01.014
Abstract:
Objective To establish a method for determining volatile components from Actinidia valvata Dunn. Methods A static headspace-gas chromatography-mass spectrometry (HS-GC-MS) method was used to analyze volatile components,and the separated peaks were identified by mass spectal library searching combined with retention index comparison. Results 42 volatile components were separated from Actinidia valvata Dunn and 25 of them were identified,mainly including alcohols,esters,aldehydes,hydrocarbons and so on. Conclusion Combined with retention index calculation,this method improved accuracy of qualitation of HS-GC-MS and provided scientific proof for the exploitation and utilization of Actinidia valvata Dunn.
Objective To establish a method for determining volatile components from Actinidia valvata Dunn. Methods A static headspace-gas chromatography-mass spectrometry (HS-GC-MS) method was used to analyze volatile components,and the separated peaks were identified by mass spectal library searching combined with retention index comparison. Results 42 volatile components were separated from Actinidia valvata Dunn and 25 of them were identified,mainly including alcohols,esters,aldehydes,hydrocarbons and so on. Conclusion Combined with retention index calculation,this method improved accuracy of qualitation of HS-GC-MS and provided scientific proof for the exploitation and utilization of Actinidia valvata Dunn.
2016, 34(1): 56-58,78.
doi: 10.3969/j.issn.1006-0111.2016.01.015
Abstract:
Objective To investigate the constituents and analgesic and anti-inflammatory effects of leaves essential oil in Schefflera octophylla. Methods SOLEO was dilated by water vapor. The mouse acetic acid-induced twisting test and mouse auricle swelling test induced by xylene were employed to evaluate the anti-inflammation and analgesia effects of SOLEO. Results 55 constituents of SOLEO were identified by GC-MS. The major constituents, 4-terpenol, (-)-spthulenol,caryophllene oxide and β-linalool with total percent of 51.86% were found. The twisting number and relieve swollen auricle of mice induced by xylene were significantly reduced by SOLEO. Conclusion SOLEO was the major active components of anti-inflammation and analgesia.
Objective To investigate the constituents and analgesic and anti-inflammatory effects of leaves essential oil in Schefflera octophylla. Methods SOLEO was dilated by water vapor. The mouse acetic acid-induced twisting test and mouse auricle swelling test induced by xylene were employed to evaluate the anti-inflammation and analgesia effects of SOLEO. Results 55 constituents of SOLEO were identified by GC-MS. The major constituents, 4-terpenol, (-)-spthulenol,caryophllene oxide and β-linalool with total percent of 51.86% were found. The twisting number and relieve swollen auricle of mice induced by xylene were significantly reduced by SOLEO. Conclusion SOLEO was the major active components of anti-inflammation and analgesia.
2016, 34(1): 59-61.
doi: 10.3969/j.issn.1006-0111.2016.01.016
Abstract:
Objective To establish the method for determination content of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC. Methods The analysis was carried on a Shim-Pack CLC-ODS column with a mobile phase of 50% acetonitrile -0.05 mol/L sodium acetate buffer (35:65) at the detection wavelength of 360 nm, using alanine and 2,4-dinitrofluorobenzene as the internal standard and derivation agent, respectively. Results The method showed a good linearity in the range of 0.006-0.030 mg/ml (r=0.999 3) for glycine. The average recovery was 101.4%, and the RSD was 0.14% (n=9). Conclusion This method was simple, sensitive, accurate, reliable, and suitable for determination of glycine in the pharmaceutical of human coagulation factor Ⅷ.
Objective To establish the method for determination content of glycine in the pharmaceutical of human coagulation factor Ⅷ by HPLC. Methods The analysis was carried on a Shim-Pack CLC-ODS column with a mobile phase of 50% acetonitrile -0.05 mol/L sodium acetate buffer (35:65) at the detection wavelength of 360 nm, using alanine and 2,4-dinitrofluorobenzene as the internal standard and derivation agent, respectively. Results The method showed a good linearity in the range of 0.006-0.030 mg/ml (r=0.999 3) for glycine. The average recovery was 101.4%, and the RSD was 0.14% (n=9). Conclusion This method was simple, sensitive, accurate, reliable, and suitable for determination of glycine in the pharmaceutical of human coagulation factor Ⅷ.
2016, 34(1): 62-65.
doi: 10.3969/j.issn.1006-0111.2016.01.017
Abstract:
Objective To compare two methods in the determination of mannatide for injection. Methods The contents were determined by spectrophotometry and HPLC method. Results In the samples determined by Spectrophotometric Determination,soluble in phenol solution and sulfuric acid, was detected at wavelength 490 nm, D-mannose derivatives linear concentration range is 10.2-51 μg/ml (r=0.999 1), the average content was 92.14%, the average RSD value was 1.17%. In the HPLC determination, with color spectrum column: Gemini C18 (250 mm×4.6 mm, 5 μm), mobile phase acetonitrile -0.02 mol/L ammonium acetate solution (20:80), flow rate:1 ml/min, the detection wavelength was at 250 nm, column temperature: 30 ℃, sample size: 10 μl detection. The average content ws 83.47%, the average RSD value was 0.65%, the content of two kinds of methods could effectively determine the D-mannose. Conclusion The average content for spectrophotometry was higher, but the phenol was used in detection of special odor, poisonous, corrosive, would cause a certain risk.HPLC was exclusive,chromatogram was more intuitive, on the operator harmfulness and less irritating. A new method for the determination of D-mannose was established at the same time.
Objective To compare two methods in the determination of mannatide for injection. Methods The contents were determined by spectrophotometry and HPLC method. Results In the samples determined by Spectrophotometric Determination,soluble in phenol solution and sulfuric acid, was detected at wavelength 490 nm, D-mannose derivatives linear concentration range is 10.2-51 μg/ml (r=0.999 1), the average content was 92.14%, the average RSD value was 1.17%. In the HPLC determination, with color spectrum column: Gemini C18 (250 mm×4.6 mm, 5 μm), mobile phase acetonitrile -0.02 mol/L ammonium acetate solution (20:80), flow rate:1 ml/min, the detection wavelength was at 250 nm, column temperature: 30 ℃, sample size: 10 μl detection. The average content ws 83.47%, the average RSD value was 0.65%, the content of two kinds of methods could effectively determine the D-mannose. Conclusion The average content for spectrophotometry was higher, but the phenol was used in detection of special odor, poisonous, corrosive, would cause a certain risk.HPLC was exclusive,chromatogram was more intuitive, on the operator harmfulness and less irritating. A new method for the determination of D-mannose was established at the same time.
2016, 34(1): 66-71.
doi: 10.3969/j.issn.1006-0111.2016.01.018
Abstract:
Objective To establish the quality standard for Quban oral liquid. Method Thin layer chromatography(TLC)was used for quality identification of Codonopsis pilosula, Astragalus membranaceus, Paeonia veitchii, Paeonia lactiflora, Atractylodes macrocephala and Angelica sinensis. The paeoniflorin was determined by RP-HPLC. Result Clear spots were obtained with good separation in TLC identification which was highly specific. The paeoniflorin area and content showed a good linear relationship at the range of 18.20-2 330.00 μg/ml (r=0.999 9). The average recovery was 99.72% (RSD=1.25%). Conclusion This method is simple, rapid, accurate, and which can be used for the quality control of Quban oral liquid.
Objective To establish the quality standard for Quban oral liquid. Method Thin layer chromatography(TLC)was used for quality identification of Codonopsis pilosula, Astragalus membranaceus, Paeonia veitchii, Paeonia lactiflora, Atractylodes macrocephala and Angelica sinensis. The paeoniflorin was determined by RP-HPLC. Result Clear spots were obtained with good separation in TLC identification which was highly specific. The paeoniflorin area and content showed a good linear relationship at the range of 18.20-2 330.00 μg/ml (r=0.999 9). The average recovery was 99.72% (RSD=1.25%). Conclusion This method is simple, rapid, accurate, and which can be used for the quality control of Quban oral liquid.
2016, 34(1): 72-75.
doi: 10.3969/j.issn.1006-0111.2016.01.019
Abstract:
Objective To improve the quality control standard of lidocaine hydrochloride injection. Methods A method for determination of related substances in lidocaine hydrochloride injection was established. Lidocaine hydrochloride was assayed by HPLC. The chromatographic conditions: C18 chromatographic column was used. The mobile phase was phosphate buffer and acetonitrile (50:50,adjusted to pH 8 with phosphoric acid). The detection wavelength was 254 nm. Results According to the result of method verification, related substances could be examined by HPLC.Lidocaine hydrochloride was assayed by HPLC, which showed excellent linearity at the range of 373.62-3 736.19 μg/ml. The average recoveries were 102.1%(RSD=0.9%). Conclusion The improved standard could be used to control the quality of lidocaine hydrochloride injection.
Objective To improve the quality control standard of lidocaine hydrochloride injection. Methods A method for determination of related substances in lidocaine hydrochloride injection was established. Lidocaine hydrochloride was assayed by HPLC. The chromatographic conditions: C18 chromatographic column was used. The mobile phase was phosphate buffer and acetonitrile (50:50,adjusted to pH 8 with phosphoric acid). The detection wavelength was 254 nm. Results According to the result of method verification, related substances could be examined by HPLC.Lidocaine hydrochloride was assayed by HPLC, which showed excellent linearity at the range of 373.62-3 736.19 μg/ml. The average recoveries were 102.1%(RSD=0.9%). Conclusion The improved standard could be used to control the quality of lidocaine hydrochloride injection.
2016, 34(1): 76-78.
doi: 10.3969/j.issn.1006-0111.2016.01.020
Abstract:
Objective To study the microorganism viability in compound resorcinol lotion, confirming with bactericidal compound resorcinol lotion. Methods 5 bottles of compound resorcinol lotion were respectively added Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Candida, Aspergillus niger, placed 24 h, then the membrane filtration method was used respectively in 5 species of microbial limit test. Results In compound resorcinol lotion, 5 microbial strains were not given birth to bacterium,were killed,the recovery rate was zero. Conclusion Microbial cannot survive in compound resorcinol lotion.
Objective To study the microorganism viability in compound resorcinol lotion, confirming with bactericidal compound resorcinol lotion. Methods 5 bottles of compound resorcinol lotion were respectively added Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Candida, Aspergillus niger, placed 24 h, then the membrane filtration method was used respectively in 5 species of microbial limit test. Results In compound resorcinol lotion, 5 microbial strains were not given birth to bacterium,were killed,the recovery rate was zero. Conclusion Microbial cannot survive in compound resorcinol lotion.
2016, 34(1): 79-82,92.
doi: 10.3969/j.issn.1006-0111.2016.01.021
Abstract:
Objective To evaluate traditional Chinese medicine (TCM) formula granules of clear heat evil and detoxification treating hyperuricemia clinical efficacy and safety through randomized, placebo controlled, blinded clinical trial. Methods 120 patients with hyperuricemia syndrome of damp heat and blood stasis were selected, randomly divided into control and treatment groups, 60 cases in each group, in a period of 4 weeks of treatment and observation. The basic treatment was given to both two groups (diet, exercise etc); in addition, the control group had placebo while treatment group was given TCM clean heat evil and detoxifying formula granules. The clinical efficacy of the products was evaluated by compariment of TCM clinical symptoms before and after treatment as well as serum uric acid,lipids (TC,TG,LDL-C),fasting blood sugar; meanwhile observing complete blood counts (CBC),liver function (ALT,AST),kidney function (BUN,Cr),electrocardiogram (ECG) and adverse reactions to evaluate its safety. Results After 4 weeks of treatment with TCM clear heat evil and detoxifying formula granules,the treatment group symptom total efficiency was 84%,while the placebo control group symptom total efficiency was 25%,the difference is significant statisticly (P<0.001); the treatment group of TCM clean heat evil and detoxifying formula granules significantly reduced in the blood uric acid,compared with before treatment (P<0.01),as well as compared with the placebo control group (P<0.05); after treatment the TCM clear heat evil and detoxifying formula granules significantly reduced in the LDL-C level (P<0.05) compared with before treatment. Comparing before treatment with after treatment,there was no significant difference between the two groups,in routine blood tests,liver and kidney functions,or ECG (P>0.05). There were no side effects or adverse events in either groups in period of treatment and observation. Conclusion TCM clear heat evil and detoxifying formula granules is curative for the hyperuricemia and obviously improve the clinical symptoms for hyperuricemia in patients with damp heat and blood stasis. It is a safe and efficient formulation to reduce uric acid.
Objective To evaluate traditional Chinese medicine (TCM) formula granules of clear heat evil and detoxification treating hyperuricemia clinical efficacy and safety through randomized, placebo controlled, blinded clinical trial. Methods 120 patients with hyperuricemia syndrome of damp heat and blood stasis were selected, randomly divided into control and treatment groups, 60 cases in each group, in a period of 4 weeks of treatment and observation. The basic treatment was given to both two groups (diet, exercise etc); in addition, the control group had placebo while treatment group was given TCM clean heat evil and detoxifying formula granules. The clinical efficacy of the products was evaluated by compariment of TCM clinical symptoms before and after treatment as well as serum uric acid,lipids (TC,TG,LDL-C),fasting blood sugar; meanwhile observing complete blood counts (CBC),liver function (ALT,AST),kidney function (BUN,Cr),electrocardiogram (ECG) and adverse reactions to evaluate its safety. Results After 4 weeks of treatment with TCM clear heat evil and detoxifying formula granules,the treatment group symptom total efficiency was 84%,while the placebo control group symptom total efficiency was 25%,the difference is significant statisticly (P<0.001); the treatment group of TCM clean heat evil and detoxifying formula granules significantly reduced in the blood uric acid,compared with before treatment (P<0.01),as well as compared with the placebo control group (P<0.05); after treatment the TCM clear heat evil and detoxifying formula granules significantly reduced in the LDL-C level (P<0.05) compared with before treatment. Comparing before treatment with after treatment,there was no significant difference between the two groups,in routine blood tests,liver and kidney functions,or ECG (P>0.05). There were no side effects or adverse events in either groups in period of treatment and observation. Conclusion TCM clear heat evil and detoxifying formula granules is curative for the hyperuricemia and obviously improve the clinical symptoms for hyperuricemia in patients with damp heat and blood stasis. It is a safe and efficient formulation to reduce uric acid.
2016, 34(1): 83-85,89.
doi: 10.3969/j.issn.1006-0111.2016.01.022
Abstract:
Objective The aim of the study is to evaluate clinical efficacy and safety of azatreonam or ceftazidime on treatment of lower respiratory tract infection. Methods Four English databases (MEDLINE、EMBASE、Pubmed、Cochrane library) and three Chinese databases (CNKI、VIP、WANFANG) were searched. Meta-analysis was performed using Review Manager 5.2. Results The Meta-analysis revealed azatreonam was superior to ceftazidime in total efficiency (RR=1.15,95% CI is 1.09-1.21). No significant differences are seen between azatreonam and ceftazidime (RR=1.03,95% CI is 0.98-1.09) on the bacterial eradication rates or the incidence of adverse reactions (RR=0.66,95% CI is 0.39-1.12). Conclusion Azatreonam is more effective than ceftazidime on the treatment of lower respiratory tract infection in the clinical practice.
Objective The aim of the study is to evaluate clinical efficacy and safety of azatreonam or ceftazidime on treatment of lower respiratory tract infection. Methods Four English databases (MEDLINE、EMBASE、Pubmed、Cochrane library) and three Chinese databases (CNKI、VIP、WANFANG) were searched. Meta-analysis was performed using Review Manager 5.2. Results The Meta-analysis revealed azatreonam was superior to ceftazidime in total efficiency (RR=1.15,95% CI is 1.09-1.21). No significant differences are seen between azatreonam and ceftazidime (RR=1.03,95% CI is 0.98-1.09) on the bacterial eradication rates or the incidence of adverse reactions (RR=0.66,95% CI is 0.39-1.12). Conclusion Azatreonam is more effective than ceftazidime on the treatment of lower respiratory tract infection in the clinical practice.
2016, 34(1): 86-89.
doi: 10.3969/j.issn.1006-0111.2016.01.023
Abstract:
Objective To investigate the application situation and analyze the main characters and developing tendency of anti-neoplastic drugs in some hospital. Methods To collect the frequency and the consumption sum of anti-neoplastic drugs used in this hospital during 2012-2013. All the data were analyzed with pharmaco-economic methods. Results Anti-neoplastic herb drugs occupied the leading position in some hospital. Kang'ai injection occupied the top of consumption sum. Ubenimex capsule occupied the top of DDDS and the bottom of DDC, most of anti-neoplastic drugs had DUI nearly 1. Conclusion The utilization of anti-neoplastic drugs was basically reasonable, herb drugs occupied the leading position in this hospital, and herb drugs played a large role in tumor treatment.
Objective To investigate the application situation and analyze the main characters and developing tendency of anti-neoplastic drugs in some hospital. Methods To collect the frequency and the consumption sum of anti-neoplastic drugs used in this hospital during 2012-2013. All the data were analyzed with pharmaco-economic methods. Results Anti-neoplastic herb drugs occupied the leading position in some hospital. Kang'ai injection occupied the top of consumption sum. Ubenimex capsule occupied the top of DDDS and the bottom of DDC, most of anti-neoplastic drugs had DUI nearly 1. Conclusion The utilization of anti-neoplastic drugs was basically reasonable, herb drugs occupied the leading position in this hospital, and herb drugs played a large role in tumor treatment.
2016, 34(1): 90-92.
doi: 10.3969/j.issn.1006-0111.2016.01.024
Abstract:
Objective To monitor the medication process of advanced lung cancer patients with renal insufficiency. Methods Pharmaceutical care, pharmaceutical intervention and medication were offered according to the individual condition of patient with renal function based on pharmaceutical experience and related literature. Results The patient finished chemotherapy successfully without adverse reaction. Conclusion Clinical pharmacists should keep improving their knowledge and participating in first-line clinical treatment activities; the patient-centered hospital pharmacy service will be founded and the level of rational medication will be improved when doctors, pharmacists and nurses could cooperate sincerely.
Objective To monitor the medication process of advanced lung cancer patients with renal insufficiency. Methods Pharmaceutical care, pharmaceutical intervention and medication were offered according to the individual condition of patient with renal function based on pharmaceutical experience and related literature. Results The patient finished chemotherapy successfully without adverse reaction. Conclusion Clinical pharmacists should keep improving their knowledge and participating in first-line clinical treatment activities; the patient-centered hospital pharmacy service will be founded and the level of rational medication will be improved when doctors, pharmacists and nurses could cooperate sincerely.
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