2013 Vol. 31, No. 2
Display Method:
2013, 31(2): 81-85.
doi: 10.3969/j.issn.1006-0111.2013.02.001
Abstract:
Hepatocellular carcinoma (HCC) was one of the most common malignancies with high incidence and mortality which threatened human health. Integrating analytical technology of high throughput and high resolution with bioinformatics, metabonomics could investigate the organism on the level of metabolism, provide a unique insight into searching biomarkers for early diagnosis of HCC and exploring the mechanism of HCC. The advances of metabonomics studies on HCC in the recent years were reviewed in this paper which could provide the reference for further research.
Hepatocellular carcinoma (HCC) was one of the most common malignancies with high incidence and mortality which threatened human health. Integrating analytical technology of high throughput and high resolution with bioinformatics, metabonomics could investigate the organism on the level of metabolism, provide a unique insight into searching biomarkers for early diagnosis of HCC and exploring the mechanism of HCC. The advances of metabonomics studies on HCC in the recent years were reviewed in this paper which could provide the reference for further research.
2013, 31(2): 86-89,115.
doi: 10.3969/j.issn.1006-0111.2013.02.002
Abstract:
Some unique inherent properties of polymeric micelles, including small particle size, high stability, long residence time, and good biocompatibility allowed polymeric micelles to be used as drug carriers. In recent years, increasing reports about the polymer micelles had been designed for tumor targeted drug delivery systems, including passive targeted drug delivery using tumor pathological nature and active targeting drug delivery using surface modification of polymer micelles. The research progress of polymeric micelles used as tumor targeted drug carriers were reviewed in this paper.
Some unique inherent properties of polymeric micelles, including small particle size, high stability, long residence time, and good biocompatibility allowed polymeric micelles to be used as drug carriers. In recent years, increasing reports about the polymer micelles had been designed for tumor targeted drug delivery systems, including passive targeted drug delivery using tumor pathological nature and active targeting drug delivery using surface modification of polymer micelles. The research progress of polymeric micelles used as tumor targeted drug carriers were reviewed in this paper.
2013, 31(2): 90-93.
doi: 10.3969/j.issn.1006-0111.2012.02.003
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In biological samples, the concentration and efficacy of drugs had great correlation, especially in the plasma (or serum), the concentration of drugs was directly related to efficacy. There was a wealth of drug metabolites in the urine, which was also frequently used. Because saliva collection convenient and sometimes it had great relevance with the plasma free drug concentration, so it was used sometimes. Other organs and tissues, except the special needs, were less used. Because of the complexity and diversity of sample preparation techniques, drug analysis of biological samples put forward higher requirements. In a variety of treatment methods, solid phase extraction attracted more and more the attention from the majority scientists because of its applicability, practicality. The reports with the application of solid phase extraction (SPE) in the analysis of biological samples were reviewed, in order to provide theoretical guidance and technical support to the relevant researchers.
In biological samples, the concentration and efficacy of drugs had great correlation, especially in the plasma (or serum), the concentration of drugs was directly related to efficacy. There was a wealth of drug metabolites in the urine, which was also frequently used. Because saliva collection convenient and sometimes it had great relevance with the plasma free drug concentration, so it was used sometimes. Other organs and tissues, except the special needs, were less used. Because of the complexity and diversity of sample preparation techniques, drug analysis of biological samples put forward higher requirements. In a variety of treatment methods, solid phase extraction attracted more and more the attention from the majority scientists because of its applicability, practicality. The reports with the application of solid phase extraction (SPE) in the analysis of biological samples were reviewed, in order to provide theoretical guidance and technical support to the relevant researchers.
2013, 31(2): 94-97,112.
doi: 10.3969/j.issn.1006-0111.2013.02.004
Abstract:
The aldehyde dehydrogenase II (ALDH2) could act as both esterase and dehydrogenase. The improvement of ALDH2 activity might reduce the damage caused by alcohol and ischemia and avoid the occurrence of some certain cancers. The outline of ALDH2 and its agonists were summarized concisely through literature analysis, collation and induction in this paper.
The aldehyde dehydrogenase II (ALDH2) could act as both esterase and dehydrogenase. The improvement of ALDH2 activity might reduce the damage caused by alcohol and ischemia and avoid the occurrence of some certain cancers. The outline of ALDH2 and its agonists were summarized concisely through literature analysis, collation and induction in this paper.
2013, 31(2): 98-101,107.
doi: 10.3969/j.issn.1006-0111.2013.02.005
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Objective To design and evaluate a multifunctional and multiple unit oral DDS, which depends on the preparations of pulsatile release mini-tablets. Methods Based on the combinations of pulsatile release tablets, zero-order DDS could be obtained. Velocity-time curve, instead of the cumulative percentage drug release profile, was plotted. The nonlinear least square model fit program was applied to process the velocity data of dissolution. The test curves coincided with the theoretical curves from simple summation of v-t equations of individual pulsatile release tablets. Results Zero-order DDS were designed with two kinds of combinations. The amount of the two pulsatile should be 1:1. The combination of pulsatile release tablet could achieved the formulation from which Acetaminophen and Pseudoephedrine could be released in the same proportion. Conclusion The combination of delayed-onset sustained-release Tablet was better fit in with zero-order equation and Acetaminophen and Pseudoephedrine could be released in the same proportion.
Objective To design and evaluate a multifunctional and multiple unit oral DDS, which depends on the preparations of pulsatile release mini-tablets. Methods Based on the combinations of pulsatile release tablets, zero-order DDS could be obtained. Velocity-time curve, instead of the cumulative percentage drug release profile, was plotted. The nonlinear least square model fit program was applied to process the velocity data of dissolution. The test curves coincided with the theoretical curves from simple summation of v-t equations of individual pulsatile release tablets. Results Zero-order DDS were designed with two kinds of combinations. The amount of the two pulsatile should be 1:1. The combination of pulsatile release tablet could achieved the formulation from which Acetaminophen and Pseudoephedrine could be released in the same proportion. Conclusion The combination of delayed-onset sustained-release Tablet was better fit in with zero-order equation and Acetaminophen and Pseudoephedrine could be released in the same proportion.
2013, 31(2): 102-107.
doi: 10.3969/j.issn.1006-0111.2013.02.006
Abstract:
Objective To design and synthesize the new 2-pyridyl ketone amide derivatives and test the antiviral activity. Methods According to the pharmacophore model, a new class of 2-pyridyl ketone amide derivatives was designed, which were synthesized by Heck reaction, selective nitration reaction, catalytic hydrogenation reaction, acylation reaction and the open-loop rearrangement reaction. All the synthesized compounds were confirmed by 1H NMR, and their anti-HBV-DNA replication activity were determined. Results The designed novel 2-pyridyl ketone amide derivatives had inhibitory activity against HBV-DNA replication. Among them, compounds 6h、6e and 6a showed the best inhibitory activity. Conclusion When the 4-ethoxyl phenyl group was attached on the N atom of the benzyl ring, the compounds revealed good inhibitory activity.
Objective To design and synthesize the new 2-pyridyl ketone amide derivatives and test the antiviral activity. Methods According to the pharmacophore model, a new class of 2-pyridyl ketone amide derivatives was designed, which were synthesized by Heck reaction, selective nitration reaction, catalytic hydrogenation reaction, acylation reaction and the open-loop rearrangement reaction. All the synthesized compounds were confirmed by 1H NMR, and their anti-HBV-DNA replication activity were determined. Results The designed novel 2-pyridyl ketone amide derivatives had inhibitory activity against HBV-DNA replication. Among them, compounds 6h、6e and 6a showed the best inhibitory activity. Conclusion When the 4-ethoxyl phenyl group was attached on the N atom of the benzyl ring, the compounds revealed good inhibitory activity.
2013, 31(2): 108-112.
doi: 10.3969/j.issn.1006-0111.2013.02.007
Abstract:
Objective To established a two-step discriminant method based on SVM and HQI or PCA, in which cephalexin capsules from several domestic manufacturers were used as tool drugs for counterfeits of brand drugs appear on the market. Method In the first step, a simulating classification model of cephalexin brand capsules and 4 kinds of counterfeit drugs was built, the cross-validation accuracy was 95.63%, and prediction accuracy of suspicious samples within the model was 93.75%, and the false-positive rate of additional homemade test samples was 25%. In the second step, the positive samples were applied to HQI and PCA methods, and the false positive rate could be reduced to 5% and 0% respectively. Results and Conclusion The two-step discrimination method established in our study could detected counterfeits of brand drugs efficiently and accurately.
Objective To established a two-step discriminant method based on SVM and HQI or PCA, in which cephalexin capsules from several domestic manufacturers were used as tool drugs for counterfeits of brand drugs appear on the market. Method In the first step, a simulating classification model of cephalexin brand capsules and 4 kinds of counterfeit drugs was built, the cross-validation accuracy was 95.63%, and prediction accuracy of suspicious samples within the model was 93.75%, and the false-positive rate of additional homemade test samples was 25%. In the second step, the positive samples were applied to HQI and PCA methods, and the false positive rate could be reduced to 5% and 0% respectively. Results and Conclusion The two-step discrimination method established in our study could detected counterfeits of brand drugs efficiently and accurately.
2013, 31(2): 113-115.
doi: 10.3969/j.issn.1006-0111.2013.02.008
Abstract:
Objective To establish a HPLC-ECD method for determination of dopamine hydrochloride in prefilled syringe. Methods The determination was performed on a Diamonsil-C18 analytical column (250 mm×4.6 mm,5 μm) with the mobile phase consisting of methanol-0.11% sodium heptanesulfonate solution (containing 0.1 mmol/L EDTA-2Na and 5% glacial acetic acid, adjusting to pH 3.4 with 1 mol/L NaOH). The silver/silver chloride electrode was used as reference electrode and the glassy carbon electrode was used as the working electrode at the potential of+0. 65 V.The flow rate was 1.0 ml/min and column temperature was 20℃. Results The calibration curve of dopamine hydrochloride were linear in the concentration range of 0.05~5 μg/ml (r=0.999 9,n=5). The average recovery rate of dopamine hydrochloride was (99.19±4.03)% (n=3). RSD of intra-day and inter-day were less than 4% (n=3). The contents of dopamine hydrochlorid in three batches of samples were(102.97±1.11)%. Conclusion The method was accurate,rapid and high sensitivity,which was suitable for the determination of dopamine hydrochloride in prefilled syringe.
Objective To establish a HPLC-ECD method for determination of dopamine hydrochloride in prefilled syringe. Methods The determination was performed on a Diamonsil-C18 analytical column (250 mm×4.6 mm,5 μm) with the mobile phase consisting of methanol-0.11% sodium heptanesulfonate solution (containing 0.1 mmol/L EDTA-2Na and 5% glacial acetic acid, adjusting to pH 3.4 with 1 mol/L NaOH). The silver/silver chloride electrode was used as reference electrode and the glassy carbon electrode was used as the working electrode at the potential of+0. 65 V.The flow rate was 1.0 ml/min and column temperature was 20℃. Results The calibration curve of dopamine hydrochloride were linear in the concentration range of 0.05~5 μg/ml (r=0.999 9,n=5). The average recovery rate of dopamine hydrochloride was (99.19±4.03)% (n=3). RSD of intra-day and inter-day were less than 4% (n=3). The contents of dopamine hydrochlorid in three batches of samples were(102.97±1.11)%. Conclusion The method was accurate,rapid and high sensitivity,which was suitable for the determination of dopamine hydrochloride in prefilled syringe.
2013, 31(2): 116-119.
doi: 10.3969/j.issn.1006-0111.2013.02.009
Abstract:
Objective To analyze chemical constituents of Pueraria lobata by ultrahigh pressure liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOFMS). Methods A ACQUITY UPLC HSS T3 (100 mm×2.1 mm, 1.8 μm) column was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.4 ml/min. TOFMS was applied for qualitative analysis under positive ion mode. Results Under optimized condition, 19 major constituents in Gardenia jasminoides Ellis were identified by quadrupole-time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using UHPLC-Q-TOFMS was established to identify the chemical constituents of Pueraria lobata.
Objective To analyze chemical constituents of Pueraria lobata by ultrahigh pressure liquid chromatography-quadrupole-time of flight mass spectrometry (UHPLC-Q-TOFMS). Methods A ACQUITY UPLC HSS T3 (100 mm×2.1 mm, 1.8 μm) column was used to separate. The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute. The flow rate was 0.4 ml/min. TOFMS was applied for qualitative analysis under positive ion mode. Results Under optimized condition, 19 major constituents in Gardenia jasminoides Ellis were identified by quadrupole-time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using UHPLC-Q-TOFMS was established to identify the chemical constituents of Pueraria lobata.
2013, 31(2): 120-123.
doi: 10.3969/j.issn.1006-0111.2013.02.010
Abstract:
Objective To study the optimal combined use of extracts from Engelhardtia roxburghiana with Rhizoma Wenyujin Concisum on acute myocardial ischemia in rats. Methods Six combined treatment groups were designed according to the weighted modification method, acute myocardial ischemia rats were induced by coronary artery ligation. The size of myocardial infarction and the levels of LDH and CK in serum were measured to evaluating acute myocardium ischemia injury. The nonlinear mixed effect model (N0NMEM) was used to quantitatively assess the effects of combined use of drugs. Results Extracts from Engelhardtia roxburghiana or Rhizoma Wenyujin Concisum could protect myocardial cells, significantly reduce serum CK and LDH in acute myocardial ischemia rats. N0NMEM analysis showed that there were obvious synergistic effects of the combination of Engelhardtia roxburghiana exctract with Rhizoma Wenyujin Concisum exctract on myocardial ischemia. Conclusion Extracts from Engelhardtia roxburghiana or from Rhizoma Wenyujin Concisum had synergistic and protective effects on acute myocardial ischemia in rats.
Objective To study the optimal combined use of extracts from Engelhardtia roxburghiana with Rhizoma Wenyujin Concisum on acute myocardial ischemia in rats. Methods Six combined treatment groups were designed according to the weighted modification method, acute myocardial ischemia rats were induced by coronary artery ligation. The size of myocardial infarction and the levels of LDH and CK in serum were measured to evaluating acute myocardium ischemia injury. The nonlinear mixed effect model (N0NMEM) was used to quantitatively assess the effects of combined use of drugs. Results Extracts from Engelhardtia roxburghiana or Rhizoma Wenyujin Concisum could protect myocardial cells, significantly reduce serum CK and LDH in acute myocardial ischemia rats. N0NMEM analysis showed that there were obvious synergistic effects of the combination of Engelhardtia roxburghiana exctract with Rhizoma Wenyujin Concisum exctract on myocardial ischemia. Conclusion Extracts from Engelhardtia roxburghiana or from Rhizoma Wenyujin Concisum had synergistic and protective effects on acute myocardial ischemia in rats.
2013, 31(2): 124-125.
doi: 10.3969/j.issn.1006-0111.2013.02.011
Abstract:
Objective To explore the infection situation and drug resistance of the non-gonococcal urethritis. Methods The samples from the patients during 2009-01/2011-03 in our hospital were sellected, and the specimens had been dealt with immediately, without exceeding 4 h at room temperature (18~25℃), or stored at 4℃ more than 48 h, which had been observed and statistically analyzed. Results The main reasons of infection and related consequences were found, and recommendations for prevention was proposed for peer reference and discussion. Conclusion Josamycin and doxycycline were recommended for the treatment of mycoplasma infection.
Objective To explore the infection situation and drug resistance of the non-gonococcal urethritis. Methods The samples from the patients during 2009-01/2011-03 in our hospital were sellected, and the specimens had been dealt with immediately, without exceeding 4 h at room temperature (18~25℃), or stored at 4℃ more than 48 h, which had been observed and statistically analyzed. Results The main reasons of infection and related consequences were found, and recommendations for prevention was proposed for peer reference and discussion. Conclusion Josamycin and doxycycline were recommended for the treatment of mycoplasma infection.
2013, 31(2): 126-128,142.
doi: 10.3969/j.issn.1006-0111.2013.02.012
Abstract:
Objective To evaluate the efficacy and explore the mechanism of Jiangtang Mixture in treatment of diabetic peripheral neuropathy (DPN). Methods 60 cases of DPN patients were randomly divided into treatment group and control group with 30 cases in each group. Two groups were all treated with a diet control, routine hypoglycemic therapy, the control group was treated with Methylcobalamin Tablet 0.5 mg, po, tid. While the treatment group was treated with Jiangtang Mixture 50 ml, po, tid, on the basis of the control group. The course of treatment was 4 weeks in each group. Before and after treatment, clinical signs and symptoms were observed and determined, including fasting plasma glucose (FPG), the 2 hours postprandial blood glucose (PPG2h), glycated hemoglobin (HbA1C), plasma viscosity (PV), endothelin (ET), nitric oxide (NO), and nerve conduction velocity (NCV). Results The total effective rate of the treatment group (86.67%) was significantly higher than that of the control group (63.33%)(P<0.05). There were significant differences in levels of the indexes, such as NCV、FPG、PPG2h、HbA1C、PV、ET and NO in the treatment group before and after treatment (P<0.05), but only in the four indexes, such as NCV、PV、ET and NO, the treatment group compared with the control group showed significantly differences after treatment (P<0.05). Adverse reactions of Jiangtang Mixture did not find in the observation period. Conclusion Jiangtang Mixture combined with Mecobalamine to treat DPN was safe and effective. The mechanism could be related to protect vascular endothelial cell, improve blood rheology, expand the peripheral microvascular, nourish the nerve with blood and oxygen supply, prevent or reverse impaired peripheral nerve fibers of DPN.
Objective To evaluate the efficacy and explore the mechanism of Jiangtang Mixture in treatment of diabetic peripheral neuropathy (DPN). Methods 60 cases of DPN patients were randomly divided into treatment group and control group with 30 cases in each group. Two groups were all treated with a diet control, routine hypoglycemic therapy, the control group was treated with Methylcobalamin Tablet 0.5 mg, po, tid. While the treatment group was treated with Jiangtang Mixture 50 ml, po, tid, on the basis of the control group. The course of treatment was 4 weeks in each group. Before and after treatment, clinical signs and symptoms were observed and determined, including fasting plasma glucose (FPG), the 2 hours postprandial blood glucose (PPG2h), glycated hemoglobin (HbA1C), plasma viscosity (PV), endothelin (ET), nitric oxide (NO), and nerve conduction velocity (NCV). Results The total effective rate of the treatment group (86.67%) was significantly higher than that of the control group (63.33%)(P<0.05). There were significant differences in levels of the indexes, such as NCV、FPG、PPG2h、HbA1C、PV、ET and NO in the treatment group before and after treatment (P<0.05), but only in the four indexes, such as NCV、PV、ET and NO, the treatment group compared with the control group showed significantly differences after treatment (P<0.05). Adverse reactions of Jiangtang Mixture did not find in the observation period. Conclusion Jiangtang Mixture combined with Mecobalamine to treat DPN was safe and effective. The mechanism could be related to protect vascular endothelial cell, improve blood rheology, expand the peripheral microvascular, nourish the nerve with blood and oxygen supply, prevent or reverse impaired peripheral nerve fibers of DPN.
2013, 31(2): 129-130,147.
doi: 10.3969/j.issn.1006-0111.2013.02.013
Abstract:
Objective To compare the methods of saturated water solution and ultrasonic for preparation inclusion compound of β-cyclodextrin with peppermint oil. Methods After extraction of peppermint oil, the inclusion compound of β-cyclodextrin with peppermint oil was prepared by saturated water solution method and ultrasonic method. Results The better inclusion method was saturated water solution with higher inclusion rate and yield of inclusion compound. And the optimum inclusion conditions were as follows:peppermint oil(ml):β-cyclodextrin(g)=1:5, stirring for 1 hour, 45℃, β-cyclodextrin(g):water(ml)=1:10. Conclusion The better inclusion method was saturated water solution which was reasonable and convenient.
Objective To compare the methods of saturated water solution and ultrasonic for preparation inclusion compound of β-cyclodextrin with peppermint oil. Methods After extraction of peppermint oil, the inclusion compound of β-cyclodextrin with peppermint oil was prepared by saturated water solution method and ultrasonic method. Results The better inclusion method was saturated water solution with higher inclusion rate and yield of inclusion compound. And the optimum inclusion conditions were as follows:peppermint oil(ml):β-cyclodextrin(g)=1:5, stirring for 1 hour, 45℃, β-cyclodextrin(g):water(ml)=1:10. Conclusion The better inclusion method was saturated water solution which was reasonable and convenient.
2013, 31(2): 131-133.
doi: 10.3969/j.issn.1006-0111.2013.02.014
Abstract:
Objective To evaluate the quality of dioxogen in the market of Jiangsu province. Methods The general quality of all samples was detected by methods of Chp 2005(VolumeⅡ) and Chp 2010(VolumeⅡ). Results Based on the standards, all of 99 samples were quality conformance. Conclusion The process technology for dioxogen was maturely established, which properties were stable and the total quality was better.
Objective To evaluate the quality of dioxogen in the market of Jiangsu province. Methods The general quality of all samples was detected by methods of Chp 2005(VolumeⅡ) and Chp 2010(VolumeⅡ). Results Based on the standards, all of 99 samples were quality conformance. Conclusion The process technology for dioxogen was maturely established, which properties were stable and the total quality was better.
2013, 31(2): 134-136.
doi: 10.3969/j.issn.1006-0111.2013.02.015
Abstract:
Objective To establish a method for determination of ursolie acid and oleanolic acid contents in Salvia chinensis Benth. Methods HPLC-DAD was used;the column was Agilent HC18 (4.6 mm×250 mm,5μm);mobile phase was A:0.1%(v/v)formic acid solution,B:methanol,A:B=14:86. The flow rate was 1.0 ml/min;the temperature of column was 20℃;the detection wavelength was set at 205 nm;the injection volume was 50 μl; the running time was 35 min. Results Ursolic acid and oleanolic acid were separated in baseline, the linear range was 11.55~462.0 μg/ml for ursolic acid and 5.265~210.6 μg/ml for oleanolic acid. The result of intra-day and inter-day precisions were both within 5%(n=3), and the average recovery was 100.47%(RSD=1.95%, n=6)and 101.88%(RSD=2.98%, n=6). Conclusion The method was rapid,convenient,accurate,and suitable for quality control of ursolic and oleanolic acid in Salvia chinensis Benth.
Objective To establish a method for determination of ursolie acid and oleanolic acid contents in Salvia chinensis Benth. Methods HPLC-DAD was used;the column was Agilent HC18 (4.6 mm×250 mm,5μm);mobile phase was A:0.1%(v/v)formic acid solution,B:methanol,A:B=14:86. The flow rate was 1.0 ml/min;the temperature of column was 20℃;the detection wavelength was set at 205 nm;the injection volume was 50 μl; the running time was 35 min. Results Ursolic acid and oleanolic acid were separated in baseline, the linear range was 11.55~462.0 μg/ml for ursolic acid and 5.265~210.6 μg/ml for oleanolic acid. The result of intra-day and inter-day precisions were both within 5%(n=3), and the average recovery was 100.47%(RSD=1.95%, n=6)and 101.88%(RSD=2.98%, n=6). Conclusion The method was rapid,convenient,accurate,and suitable for quality control of ursolic and oleanolic acid in Salvia chinensis Benth.
2013, 31(2): 137-139.
doi: 10.3969/j.issn.1006-0111.2013.02.016
Abstract:
Objective To establish the fingerprint analysis for quality evaluation index of of Chuanshentong injection. Methods The fingerprints were established by HPLC. The mobile phase was acetonitrile-0.1% aqueous Formic acid solution in a gradient elution mode. The column temperature was maintained at 35℃. The flow rate was 1.0 ml /min,and detection wavelength was set at 281 nm. Results 11 common peaks were obtained in the fingerprint of 11 batches of Chuanshentong injection with the help of peaks match with Similarity Evaluation System for Chromatographic Fingerprint of TCM(Version 2004A). The similarity among 11 batches of Chuanshentong Injection was calculated more than 0.970.Principal components analyses was carried out to study the common HPLC fingerprint. Conclusion The method was simple,accurate and sensitive which could be used to evaluate the quality of Chuanshentong injection.
Objective To establish the fingerprint analysis for quality evaluation index of of Chuanshentong injection. Methods The fingerprints were established by HPLC. The mobile phase was acetonitrile-0.1% aqueous Formic acid solution in a gradient elution mode. The column temperature was maintained at 35℃. The flow rate was 1.0 ml /min,and detection wavelength was set at 281 nm. Results 11 common peaks were obtained in the fingerprint of 11 batches of Chuanshentong injection with the help of peaks match with Similarity Evaluation System for Chromatographic Fingerprint of TCM(Version 2004A). The similarity among 11 batches of Chuanshentong Injection was calculated more than 0.970.Principal components analyses was carried out to study the common HPLC fingerprint. Conclusion The method was simple,accurate and sensitive which could be used to evaluate the quality of Chuanshentong injection.
2013, 31(2): 140-142.
doi: 10.3969/j.issn.1006-0111.2013.02.017
Abstract:
Objective To establish a RP-HPLC method for the determination of hydroxysaffor yellows A in Yihe decoction. Methods The separation was performed on a Yilite C18 column(250 mm×4.6 mm, 5 μm). The mobile phase was methanol-0.5% acetic acid(20:80) with a flow rate of 1.0 ml/min. The detection wavelength was set at 403 nm. Results Hydroxysaffor yellow A in the range of 0.816 0~32.64 μg/ml showed a good linear relationship (r=0.999 9). The average recovery was 101.0% and RSD was 1.8% (n=6). Conclusion This accurate and reliable HPLC method could be used for the determination of hydroxysaffor yellow A in Yihe decoction.
Objective To establish a RP-HPLC method for the determination of hydroxysaffor yellows A in Yihe decoction. Methods The separation was performed on a Yilite C18 column(250 mm×4.6 mm, 5 μm). The mobile phase was methanol-0.5% acetic acid(20:80) with a flow rate of 1.0 ml/min. The detection wavelength was set at 403 nm. Results Hydroxysaffor yellow A in the range of 0.816 0~32.64 μg/ml showed a good linear relationship (r=0.999 9). The average recovery was 101.0% and RSD was 1.8% (n=6). Conclusion This accurate and reliable HPLC method could be used for the determination of hydroxysaffor yellow A in Yihe decoction.
2013, 31(2): 143-144,150.
doi: 10.3969/j.issn.1006-0111.2013.02.018
Abstract:
Objective To investigate economic effects of three kinds of drugs in treatment of osteoporosis. Methods 135 cases were divided into three groups, A group with alendronate sodium and vitamin D3 tablets 70 mg/2 800 IU, once a week, early morning,B group with salmon calcitonin nasal spray, once a day every time 100 IU, C group with zoledronicacid injection 4 mg, once a year. All groups were treated for one year, three groups of patients were added calcium at the same time. Efficacy was observed. Cost-effectiveness analysis was used. Results In the improvement of bone pain, A group was 66.67%, B group was 55.56%, C group was 80%. C group was higher than those in group A, group B, group efficacy was statistically significant in three groups(P<0.05); The effectiveness ratio of A group, B group, C group was 59.52,43.61,46.70 respectively. B group was superior to A group and C group in the terms of economic, but group B had the lowest efficacy in all groups. Conclusion C group was the optimal solution for osteoporosis.
Objective To investigate economic effects of three kinds of drugs in treatment of osteoporosis. Methods 135 cases were divided into three groups, A group with alendronate sodium and vitamin D3 tablets 70 mg/2 800 IU, once a week, early morning,B group with salmon calcitonin nasal spray, once a day every time 100 IU, C group with zoledronicacid injection 4 mg, once a year. All groups were treated for one year, three groups of patients were added calcium at the same time. Efficacy was observed. Cost-effectiveness analysis was used. Results In the improvement of bone pain, A group was 66.67%, B group was 55.56%, C group was 80%. C group was higher than those in group A, group B, group efficacy was statistically significant in three groups(P<0.05); The effectiveness ratio of A group, B group, C group was 59.52,43.61,46.70 respectively. B group was superior to A group and C group in the terms of economic, but group B had the lowest efficacy in all groups. Conclusion C group was the optimal solution for osteoporosis.
2013, 31(2): 145-147.
doi: 10.3969/j.issn.1006-0111.2013.02.019
Abstract:
Objective To discuss the role of clinical thinking by clinical pharmacists in clinical practice. Methods With the aid of clinical thinking,a series of clinical practice including medical history collection, drugs use education treatment schemes,analysis, pharmaceutical care and adverse reaction analysis were carried out by clinical pharmacists. Results Clinical pharmacists got better opportunities in involving patients treatment, optimizing drug choice, which could help patients to improve compliance and safety consciousness of taking medicine. Conclusion It was important for Clinical thinking by clinical pharmacists to take part in clinical practice.
Objective To discuss the role of clinical thinking by clinical pharmacists in clinical practice. Methods With the aid of clinical thinking,a series of clinical practice including medical history collection, drugs use education treatment schemes,analysis, pharmaceutical care and adverse reaction analysis were carried out by clinical pharmacists. Results Clinical pharmacists got better opportunities in involving patients treatment, optimizing drug choice, which could help patients to improve compliance and safety consciousness of taking medicine. Conclusion It was important for Clinical thinking by clinical pharmacists to take part in clinical practice.
2013, 31(2): 148-150.
doi: 10.3969/j.issn.1006-0111.2013.02.020
Abstract:
Objective To reduce medical disputes in outpatient pharmacy for drug procurement mistake, improve the work efficiency, and offer the reference for the performance analysis in outpatient pharmacy. Methods The accurate drug preparation system for outpatient pharmacy was jointly designed and developed by the 302nd Hospital of PLA and Kang Lian Da(Beijing) software Co., LTD.,which was used to accomplish the working process of drugs preparation. Results Pharmacist used the system to prepare drugs. The preparation time was shorten and the accuracy was improved. The system's software recorded the workload of drugs preparation. Conclusion The system was simple and easy to operate, which was suitable for the promotion in hospital pharmacy.
Objective To reduce medical disputes in outpatient pharmacy for drug procurement mistake, improve the work efficiency, and offer the reference for the performance analysis in outpatient pharmacy. Methods The accurate drug preparation system for outpatient pharmacy was jointly designed and developed by the 302nd Hospital of PLA and Kang Lian Da(Beijing) software Co., LTD.,which was used to accomplish the working process of drugs preparation. Results Pharmacist used the system to prepare drugs. The preparation time was shorten and the accuracy was improved. The system's software recorded the workload of drugs preparation. Conclusion The system was simple and easy to operate, which was suitable for the promotion in hospital pharmacy.
2013, 31(2): 151-153,160.
doi: 10.3969/j.issn.1006-0111.2013.02.021
Abstract:
Social & Administrative Pharmacy was an emerging inter-disciplinary,the postgraduate education of which was growing vigorously in recent years. The situation of the domestic and foreign-disciplinary postgraduate cultivation was compared in this paper, a larger proportion of foreign social pharmacy education was not only more biased in favor of the pharmacy practice education, humanities and social science education, but also stressed the initiative of the people in the pharmacy services. Therefore, a series of measures to build a scientific and reasonable teaching system were proposed, such as taking the science of developing talents and goals, completing the perfect curriculum system, intensifying the construction of teaching materials, and strengthening the teaching staff, introducing the different teaching methods, which could provide ideas for postgraduate cultivation.
Social & Administrative Pharmacy was an emerging inter-disciplinary,the postgraduate education of which was growing vigorously in recent years. The situation of the domestic and foreign-disciplinary postgraduate cultivation was compared in this paper, a larger proportion of foreign social pharmacy education was not only more biased in favor of the pharmacy practice education, humanities and social science education, but also stressed the initiative of the people in the pharmacy services. Therefore, a series of measures to build a scientific and reasonable teaching system were proposed, such as taking the science of developing talents and goals, completing the perfect curriculum system, intensifying the construction of teaching materials, and strengthening the teaching staff, introducing the different teaching methods, which could provide ideas for postgraduate cultivation.
2013, 31(2): 154-157.
doi: 10.3969/j.issn.1006-0111.2013.02.022
Abstract:
With innovation education as the goal, general education as the core, the status of undergraduate innovative experiment training in China and in American were summarized in this study, the organization structure, management mechanism, project selection and exchange activities of undergraduate innovative experiment training were researched. By using the general education and innovation education theory, the training system "innovative experiment core curriculum combined with extracurricular innovative scientific research project"were constructed. The teaching strategy "with students as the main body of learning and scientific research experience" was promoted. A set of more scientific guidance was formed, theoretical and practical basis were provided for undergraduates innovation experiment training. The important role of experiment teaching center and innovation experiment training organization in the cultivation of innovative talents were expressed.
With innovation education as the goal, general education as the core, the status of undergraduate innovative experiment training in China and in American were summarized in this study, the organization structure, management mechanism, project selection and exchange activities of undergraduate innovative experiment training were researched. By using the general education and innovation education theory, the training system "innovative experiment core curriculum combined with extracurricular innovative scientific research project"were constructed. The teaching strategy "with students as the main body of learning and scientific research experience" was promoted. A set of more scientific guidance was formed, theoretical and practical basis were provided for undergraduates innovation experiment training. The important role of experiment teaching center and innovation experiment training organization in the cultivation of innovative talents were expressed.
2013, 31(2): 158-160.
doi: 10.3969/j.issn.1006-0111.2013.02.023
Abstract:
Objective To discuss the current mode of pharmaceutical high education by visiting School of Pharmacy in Southern California University. Methods The pharmacy education system in the School of pharmacy in Southern California University was introduced. Results Education system for Pharm.D. had become the core task of the U.S. pharmacy colleges. Conclusion the transformation from current pharmacy education to advanced pharmacy education system should be accelerated in China.
Objective To discuss the current mode of pharmaceutical high education by visiting School of Pharmacy in Southern California University. Methods The pharmacy education system in the School of pharmacy in Southern California University was introduced. Results Education system for Pharm.D. had become the core task of the U.S. pharmacy colleges. Conclusion the transformation from current pharmacy education to advanced pharmacy education system should be accelerated in China.
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