2008 Vol. 26, No. 1
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2008, (1): 5-6.
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2008, (1): 7-10.
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2008, (1): 11-13,37.
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2008, (1): 14-16,19.
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2008, (1): 17-19.
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Objective: To build up a fast determination method for undeclared prescription drugs in Chinese herbal medicines by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods: Multiple reaction monitoring (MRM) mode was employed for the analysis of ten possible prescription drugs, including glibenclamide, sibutramine, etc, in 36 Chinese herbal medicines which were categorized into antidiabetics, antihypertensives, aphrodisiacs, weight reducers, and others, according to pharmaceutical functions. Results: Four antidiabetics (containing glibenclamide), one aphrodisiac (containing sildenafil) and two weight reducers (containing sibutramine) were detected within 10min even without thorough chromatographic separation. Conclusion: LC-MS/MS with MRM mode guaranteed sufficient specificity for qualitative analysis and sensitivity for quantitative analysis, providing an alternative for the rapid analysis of multiple undeclared prescription drugs in Chinese herbal medicines.
Objective: To build up a fast determination method for undeclared prescription drugs in Chinese herbal medicines by liquid chromatography-tandem mass spectrometry (LC-MS/MS). Methods: Multiple reaction monitoring (MRM) mode was employed for the analysis of ten possible prescription drugs, including glibenclamide, sibutramine, etc, in 36 Chinese herbal medicines which were categorized into antidiabetics, antihypertensives, aphrodisiacs, weight reducers, and others, according to pharmaceutical functions. Results: Four antidiabetics (containing glibenclamide), one aphrodisiac (containing sildenafil) and two weight reducers (containing sibutramine) were detected within 10min even without thorough chromatographic separation. Conclusion: LC-MS/MS with MRM mode guaranteed sufficient specificity for qualitative analysis and sensitivity for quantitative analysis, providing an alternative for the rapid analysis of multiple undeclared prescription drugs in Chinese herbal medicines.
2008, (1): 20-22,47.
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Objective: To select optimal diameter of the powder of Radix Astragali and extraction method by four methods of extracting astragalus polysaccharide and total astragalus saponins in three Radix Astragali powders of different diameter. Methods: The water-alcohol co-distillation, cellulose degradation technology, calcarea solution technology, ultrasonic waves technology were used to three Radix Astragali powders of different diameter respectively.The astragalus polysaccharide and total astragalus saponins were separated with combined techniques hollow fiber membrane and D21 macro absorbing resin. According to the contents of astragalus polysaccharide and total astragalus saponins,the extraction methods were compared. Results: The most effective way of extracting astragalus polysaccharide was the ultrasonic waves technology with the yield rate of 1.73%, while the most effective method of distilling total astragalus saponins was ultrasonic waves technology on prepared slice of herbal drug, with the yield of 1.82. Conclusion: Ultrasonic waves technology was a much better way of extracting both astragalus polysaccharide and total astragalus saponins. The shorter the diameter of Radix Astragali powder, the higher the yield of extracting astragalus polysaccharide. The yield of extracting total astragalus saponins turned out contray.
Objective: To select optimal diameter of the powder of Radix Astragali and extraction method by four methods of extracting astragalus polysaccharide and total astragalus saponins in three Radix Astragali powders of different diameter. Methods: The water-alcohol co-distillation, cellulose degradation technology, calcarea solution technology, ultrasonic waves technology were used to three Radix Astragali powders of different diameter respectively.The astragalus polysaccharide and total astragalus saponins were separated with combined techniques hollow fiber membrane and D21 macro absorbing resin. According to the contents of astragalus polysaccharide and total astragalus saponins,the extraction methods were compared. Results: The most effective way of extracting astragalus polysaccharide was the ultrasonic waves technology with the yield rate of 1.73%, while the most effective method of distilling total astragalus saponins was ultrasonic waves technology on prepared slice of herbal drug, with the yield of 1.82. Conclusion: Ultrasonic waves technology was a much better way of extracting both astragalus polysaccharide and total astragalus saponins. The shorter the diameter of Radix Astragali powder, the higher the yield of extracting astragalus polysaccharide. The yield of extracting total astragalus saponins turned out contray.
2008, (1): 23-27,50.
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Objective: To investigate the effects of Dehydrocavidine (YHL-DC) on carbon tetrachloride (CCl4) induced acute liver injury in mice. Methods: The mice were pre-treatment with YHL-DC prior to the administration of CCl4 and post-treatment with YHL-DC after the administration of CCl4, and the changes of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBIL) in these mice were examined respectively. Histopathology changes of liver were also observed under both light and electron microscope. Results: Pre-treatment and post-treatment with YHL-DC significantly prevented increase in serum enzymatic activities of ALT, AST and TBIL in a dose-dependent manner. Hepatocyte degeneration and necrosis, infiltration of inflammatory cells and destruction of hepatocyte ultrastructure appeared in carbon tetrachloride induced acute injured liver in mice, while extenuations were observed in both pre-and post-treatment groups administered with YHL-DC. Conclusion: The results suggest that YHL-DC has a potent hepatoprotective action on CCl4 induced acute liver injury in mice.
Objective: To investigate the effects of Dehydrocavidine (YHL-DC) on carbon tetrachloride (CCl4) induced acute liver injury in mice. Methods: The mice were pre-treatment with YHL-DC prior to the administration of CCl4 and post-treatment with YHL-DC after the administration of CCl4, and the changes of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBIL) in these mice were examined respectively. Histopathology changes of liver were also observed under both light and electron microscope. Results: Pre-treatment and post-treatment with YHL-DC significantly prevented increase in serum enzymatic activities of ALT, AST and TBIL in a dose-dependent manner. Hepatocyte degeneration and necrosis, infiltration of inflammatory cells and destruction of hepatocyte ultrastructure appeared in carbon tetrachloride induced acute injured liver in mice, while extenuations were observed in both pre-and post-treatment groups administered with YHL-DC. Conclusion: The results suggest that YHL-DC has a potent hepatoprotective action on CCl4 induced acute liver injury in mice.
2008, (1): 28-30.
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Objective: To study the effects of ribonucleic acid Ⅱ on murine immune functions. Methods: Kunming mice （6-8 weeks old） were administered with ribonucleic acid II at dosages of 0.2,1,5,25 mg/kg respectively.Spleen and thymus were weighted and cell-mediated immune functions,humoral immune functions, delayed type hypersensitivity reaction and macrophage function assays were performed by conventional methods. Results: In comparison with control group （0.24±0.09 mm）, 0.2,1,5,25 mg/kg ribonucleic acid Ⅱ increased the foot pad swelling to 0.34±0.18, 0.47±0.14, 0.53±0.17, 0.52±0.20 mm respectively. Lymphocyte proliferation capacity was also enhanced.IgM-PFC number increased significantly to 3 444±842, 4 652±1 114, 5 656±1 569, and 6 560±1 812 per 5×106 spleen cell respectively at the dose of 0.2,1,5,25 mg/kg,significantly higher than that of control.Also, phagocytosis capacity was significantly enhanced. Conclusion: Ribonucleic Acid Ⅱ could improve the immune functions in mice.
Objective: To study the effects of ribonucleic acid Ⅱ on murine immune functions. Methods: Kunming mice （6-8 weeks old） were administered with ribonucleic acid II at dosages of 0.2,1,5,25 mg/kg respectively.Spleen and thymus were weighted and cell-mediated immune functions,humoral immune functions, delayed type hypersensitivity reaction and macrophage function assays were performed by conventional methods. Results: In comparison with control group （0.24±0.09 mm）, 0.2,1,5,25 mg/kg ribonucleic acid Ⅱ increased the foot pad swelling to 0.34±0.18, 0.47±0.14, 0.53±0.17, 0.52±0.20 mm respectively. Lymphocyte proliferation capacity was also enhanced.IgM-PFC number increased significantly to 3 444±842, 4 652±1 114, 5 656±1 569, and 6 560±1 812 per 5×106 spleen cell respectively at the dose of 0.2,1,5,25 mg/kg,significantly higher than that of control.Also, phagocytosis capacity was significantly enhanced. Conclusion: Ribonucleic Acid Ⅱ could improve the immune functions in mice.
2008, (1): 31-34.
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Objective: To study the safety of Rupixiao patch. Methods: Skin irritation tests were carried on the integral and damaged skin of healht rabbits. The skin hypersensitive test and chronic percutaneous toxicity test were carried on guinea pigs and healthy rats.Results and Conclusion: Irritation,hypersensitiveness and toxicity didn't happened on the rabbits, guinea pigs and rats, respectively.
Objective: To study the safety of Rupixiao patch. Methods: Skin irritation tests were carried on the integral and damaged skin of healht rabbits. The skin hypersensitive test and chronic percutaneous toxicity test were carried on guinea pigs and healthy rats.Results and Conclusion: Irritation,hypersensitiveness and toxicity didn't happened on the rabbits, guinea pigs and rats, respectively.
2008, (1): 35-37.
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Objective: To establish a new method for the determination of rabeprazole. Methods: Anodic adsorptive voltammetry with a carbon paste electeode(CPE) was used. Rabeprazole was concentrated on the electrode and determined by adsorptive voltammetry in supporting solution of 1.8 mol/L H2SO4. Results: The anthodic peak potential of raberazole was at 0.498 V(vs.SCE). The peak currents were linear with the concentration of rabeprazole over the range of 4.15×10-7～3.73×10--6mol/L, with the detection limit 6.705×10--11 mol/L. Conclusion: This method is suitable for the determination of rabeprazole, and it is covenient and accurate.
Objective: To establish a new method for the determination of rabeprazole. Methods: Anodic adsorptive voltammetry with a carbon paste electeode(CPE) was used. Rabeprazole was concentrated on the electrode and determined by adsorptive voltammetry in supporting solution of 1.8 mol/L H2SO4. Results: The anthodic peak potential of raberazole was at 0.498 V(vs.SCE). The peak currents were linear with the concentration of rabeprazole over the range of 4.15×10-7～3.73×10--6mol/L, with the detection limit 6.705×10--11 mol/L. Conclusion: This method is suitable for the determination of rabeprazole, and it is covenient and accurate.
2008, (1): 38-40,60.
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Objective: To establish a quantitative analysis method for berberine hydrochloride and total alkaloids in compound Er-xian decoction. Methods: Berberine hydrochloride were determined by HPLC. Using Hypersil-BDS C18 (4.6 x 200 mm) column, methanol-0.02 mol/L sodium dihydrogen phosphate-0.1 mol/L triethylamine (40-60-2) as the mobile phase. The detection wavelength was 345 nm. Total alkaloids were determined at 420 nm by visible spectrometry. Results: The linearity range of berberine hydrochloride was 0.51～10.10 μg/mL, r=0.999 9, and average recovery was 100.07%,RSD 0.4%. The linearity range of total alkaloids was 8.0～24.0 μg/mL, r=0.999 8, and average recovery was 100.46%, RSD 0.58%. Conclusions: The method is simple, quick and accurate.It can be used to control the quality of the decoction preparation.
Objective: To establish a quantitative analysis method for berberine hydrochloride and total alkaloids in compound Er-xian decoction. Methods: Berberine hydrochloride were determined by HPLC. Using Hypersil-BDS C18 (4.6 x 200 mm) column, methanol-0.02 mol/L sodium dihydrogen phosphate-0.1 mol/L triethylamine (40-60-2) as the mobile phase. The detection wavelength was 345 nm. Total alkaloids were determined at 420 nm by visible spectrometry. Results: The linearity range of berberine hydrochloride was 0.51～10.10 μg/mL, r=0.999 9, and average recovery was 100.07%,RSD 0.4%. The linearity range of total alkaloids was 8.0～24.0 μg/mL, r=0.999 8, and average recovery was 100.46%, RSD 0.58%. Conclusions: The method is simple, quick and accurate.It can be used to control the quality of the decoction preparation.
2008, (1): 41-42.
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Objective: By determining fourteen trace elements including Cu,Zn,Mn,Se,Mo,Fe,Cr,Ni,V,Sn,Pb,Cd,Hg in Shenqifuzheng injection,a kind of traditional Chinese medicine ,the regularity of the distribution of these elements in the injection was discussed. Methods: The digested samples were determined by ICP-MS. Results: The concentrations of Cu,Fe,Zn,Cr and Se in Shenqifuzheng injection were higher than those of other elements. In four poisonous elements Pb,Cd,Hg and As, the concentration of As was close to the standard of FAD about drugs and functional foods. The concentrations of Pb、Cd and Hg were all obviously lower than the highest dose limit. Conclusion: The results provide useful data for discussing the relationship between trace elements in traditional Chinese medicine and the effect of tumor -treating.
Objective: By determining fourteen trace elements including Cu,Zn,Mn,Se,Mo,Fe,Cr,Ni,V,Sn,Pb,Cd,Hg in Shenqifuzheng injection,a kind of traditional Chinese medicine ,the regularity of the distribution of these elements in the injection was discussed. Methods: The digested samples were determined by ICP-MS. Results: The concentrations of Cu,Fe,Zn,Cr and Se in Shenqifuzheng injection were higher than those of other elements. In four poisonous elements Pb,Cd,Hg and As, the concentration of As was close to the standard of FAD about drugs and functional foods. The concentrations of Pb、Cd and Hg were all obviously lower than the highest dose limit. Conclusion: The results provide useful data for discussing the relationship between trace elements in traditional Chinese medicine and the effect of tumor -treating.
2008, (1): 43-45.
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Objective: To compare the prophylactic efficacy of ondansetron,granisetron,and tropisetron on vomiting caused by TACE. Methods: 120 patients(with primary hepatocellular carcinoma) were randomly divided into four groups to receive ondanstron,granisetron,tropisetron via liver artery injection (n=30) and combined with metoclopramide as controls (n=30).The antiemetic efficacies were compared among the four groups for 5 consecutive days after TACE. Results: The effective rate of tropisetron group was significantly higher than that of the control group (P<0.05). Conclusion: Tropisetron is an effective antiemetic drug in controlling vomiting caused by TACE and should be advocated in clinical practice.
Objective: To compare the prophylactic efficacy of ondansetron,granisetron,and tropisetron on vomiting caused by TACE. Methods: 120 patients(with primary hepatocellular carcinoma) were randomly divided into four groups to receive ondanstron,granisetron,tropisetron via liver artery injection (n=30) and combined with metoclopramide as controls (n=30).The antiemetic efficacies were compared among the four groups for 5 consecutive days after TACE. Results: The effective rate of tropisetron group was significantly higher than that of the control group (P<0.05). Conclusion: Tropisetron is an effective antiemetic drug in controlling vomiting caused by TACE and should be advocated in clinical practice.
2008, (1): 45-47.
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2008, (1): 48-50.
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Objective: To develop a RP-HPLC method for determination of chlorhexidine acetate in appliance preserving solution. Methods: RP-HPLC was used for the determination of chlorhexidine acetate in appliance's preserving solution on a Shim-pack CLC-C8 column, with acetonitrile-water （including 1% triethylamine,adjecting pH to 4.0 by glacial acetate）（42-58）as mobile phase.The detection wavelength was 258 nm,flow rate was 1.0 mL/min, column temperature was 30 ℃,sample injection volume was 20 μL. Results: Chlorhexidine acetate had a good linear coefficient （r=0.999 8） over the range of 20-80 μg/mL.The average recoveries of chlorhexidine acetate was 99.96%,RSD=1.71% （n=6）. Conclusion: This method was quick and accurate for determination of chlorhexidine acetate in appliance preserving solution.
Objective: To develop a RP-HPLC method for determination of chlorhexidine acetate in appliance preserving solution. Methods: RP-HPLC was used for the determination of chlorhexidine acetate in appliance's preserving solution on a Shim-pack CLC-C8 column, with acetonitrile-water （including 1% triethylamine,adjecting pH to 4.0 by glacial acetate）（42-58）as mobile phase.The detection wavelength was 258 nm,flow rate was 1.0 mL/min, column temperature was 30 ℃,sample injection volume was 20 μL. Results: Chlorhexidine acetate had a good linear coefficient （r=0.999 8） over the range of 20-80 μg/mL.The average recoveries of chlorhexidine acetate was 99.96%,RSD=1.71% （n=6）. Conclusion: This method was quick and accurate for determination of chlorhexidine acetate in appliance preserving solution.
2008, (1): 51-53.
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Objective: To develop a HPLC method for determining bergeninum in Yinxian Tongfeibao granules. Methods: Zorbax Eclipse XDB-C18 column was used and methanol-water-acetic acid glacial（18-81-1） was used as mobil phase,detection wavelegth was 275 nm,the flow rate was 0.8 mL/min,column temperature was 30 ℃. Results: There was a good linear relationship in the range of 0.43-2.15 μg, r=0.999 88.The average recovery was 98.33% with RSD=2.68%. Conclusion: The method is rapid ,accurate and repeatable.It can be applied to an effective control of the quantity of Yinxian Tongfeibao granules.
Objective: To develop a HPLC method for determining bergeninum in Yinxian Tongfeibao granules. Methods: Zorbax Eclipse XDB-C18 column was used and methanol-water-acetic acid glacial（18-81-1） was used as mobil phase,detection wavelegth was 275 nm,the flow rate was 0.8 mL/min,column temperature was 30 ℃. Results: There was a good linear relationship in the range of 0.43-2.15 μg, r=0.999 88.The average recovery was 98.33% with RSD=2.68%. Conclusion: The method is rapid ,accurate and repeatable.It can be applied to an effective control of the quantity of Yinxian Tongfeibao granules.
2008, (1): 53-54,68.
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