2005 Vol. 23, No. 4
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2005, (4): @-@.
Abstract:
Objective To study the the effect of complex shark cartilage on immune function in tumor-bearing mice. Methods The carbon clearance, DBFB delayed hypersensitivity, and immune organs were examined in tumor-bearing BALB/C mice.Results Complex shark cartilage enhanced significantly the carbon clearance and DBFB delayed hypersensitivity and markedly increased the index of spleen and thymus in a dose-dependent manner.Conclusion complex shark cartilage may enhance the suppressed immune function in tumor-bearing mice.
Objective To study the the effect of complex shark cartilage on immune function in tumor-bearing mice. Methods The carbon clearance, DBFB delayed hypersensitivity, and immune organs were examined in tumor-bearing BALB/C mice.Results Complex shark cartilage enhanced significantly the carbon clearance and DBFB delayed hypersensitivity and markedly increased the index of spleen and thymus in a dose-dependent manner.Conclusion complex shark cartilage may enhance the suppressed immune function in tumor-bearing mice.
2005, (4): @-@.
Abstract:
Objective To investigate the espression of BP1 gene after estrogen and tamoxifen effected on breast cancer cell line MCF-7 respectively.Methods; With screened concentration of estrogen (10-8 mol/L) and tamoxifen (10-6 mol/L) by MTT assay treated with MCF-7 72 hours,then detected the expressions of BP1 mRNA by in situ hybridization method and bcl-2 protein by immuno-histoc-hemistry.Determined quantitatively apoptosis cells of MCF-7 by TUNEL method.Results The percents of BP1 mRNA espression of MCF-7 cells in E2 treated group and TAM treated group were (95.0%±3.94% ) and (58.5%±5.21% ), and those of bcl-2 protein were (80.9%±1.73%) and (37.8%±2.39%), howere, the expressions of BP1 and bcl-2 were (76.1%±6.54%) and (63.5%±3.28%) in control group respectively.There were all significant difference between each two groups (P<0.05 ).There was a significant difference in apoptosis index in either group treated with E2 (0.67±0.27) or TAM (6.7±0.76) in comparing with that of the control group (3.2±0.79) (P<0.05).Conclusion The expression of BP1 mRNA was possibly regulated by ER mediated signal transduction pathway in ER positive breast carcinoma cell line, and BP1 might play an important role in tumorgenesis of breast carcinoma through up-regulate espression mediated by E2 and apoptosis inhibition.BP1 might serve as a poor prognostic markers of breast carcinoma.
Objective To investigate the espression of BP1 gene after estrogen and tamoxifen effected on breast cancer cell line MCF-7 respectively.Methods; With screened concentration of estrogen (10-8 mol/L) and tamoxifen (10-6 mol/L) by MTT assay treated with MCF-7 72 hours,then detected the expressions of BP1 mRNA by in situ hybridization method and bcl-2 protein by immuno-histoc-hemistry.Determined quantitatively apoptosis cells of MCF-7 by TUNEL method.Results The percents of BP1 mRNA espression of MCF-7 cells in E2 treated group and TAM treated group were (95.0%±3.94% ) and (58.5%±5.21% ), and those of bcl-2 protein were (80.9%±1.73%) and (37.8%±2.39%), howere, the expressions of BP1 and bcl-2 were (76.1%±6.54%) and (63.5%±3.28%) in control group respectively.There were all significant difference between each two groups (P<0.05 ).There was a significant difference in apoptosis index in either group treated with E2 (0.67±0.27) or TAM (6.7±0.76) in comparing with that of the control group (3.2±0.79) (P<0.05).Conclusion The expression of BP1 mRNA was possibly regulated by ER mediated signal transduction pathway in ER positive breast carcinoma cell line, and BP1 might play an important role in tumorgenesis of breast carcinoma through up-regulate espression mediated by E2 and apoptosis inhibition.BP1 might serve as a poor prognostic markers of breast carcinoma.
2005, (4): @-@.
Abstract:
Objective To research the mechanism of Weiyan Keli (WK) in treatment of chronic atrophic gastritis (CAG).Methods CAG model of rats was made by synthetical methods.The rats were randomly divided into normal control group, model group and treatment group.The weight and pathohistologic change of CAG in the rats were observed.Results After treatment with WK for 10 weeks, the change of the weight in the treatment group was not significantly different from that in the model group.Epithelial cellular morphosis and atrophic degree of gastro mucosal gland were obviously improved.Inflammation cells were reduced significantly.Conclusion WK has the satisfactory effect in treating CAG.
Objective To research the mechanism of Weiyan Keli (WK) in treatment of chronic atrophic gastritis (CAG).Methods CAG model of rats was made by synthetical methods.The rats were randomly divided into normal control group, model group and treatment group.The weight and pathohistologic change of CAG in the rats were observed.Results After treatment with WK for 10 weeks, the change of the weight in the treatment group was not significantly different from that in the model group.Epithelial cellular morphosis and atrophic degree of gastro mucosal gland were obviously improved.Inflammation cells were reduced significantly.Conclusion WK has the satisfactory effect in treating CAG.
2005, (4): @-210,223.
Abstract:
Objective To prepare and determinate the ketoconazole(KCZ)transfersomes(TF),and to study the enhancing effect on the transdermal absorption of ketoconazole transfersomes.Methods The rotary evaporation method was used for preparation of KCZ TF.The HPLC was employed in its determination.The excised rabbit skin was used as the percutaneous barrier, and the modified franz-diffusion cells were used in the test.The enhancement effect of common nano-liposome, transfersomes, 3% dipentene and 3% azone were compared in the transdermal absorption of ketoconazole.Results The KCZ TF were prepared.The sequence of enhancement at difference transdermal enhancer are follows: transfersomes > 3% dipentene > 3% azone > common nana-liposome.Conclusion It was showed that various transder enhancers could promote absorption of ketoconazole in different degrees and transfersomes was the most effective.
Objective To prepare and determinate the ketoconazole(KCZ)transfersomes(TF),and to study the enhancing effect on the transdermal absorption of ketoconazole transfersomes.Methods The rotary evaporation method was used for preparation of KCZ TF.The HPLC was employed in its determination.The excised rabbit skin was used as the percutaneous barrier, and the modified franz-diffusion cells were used in the test.The enhancement effect of common nano-liposome, transfersomes, 3% dipentene and 3% azone were compared in the transdermal absorption of ketoconazole.Results The KCZ TF were prepared.The sequence of enhancement at difference transdermal enhancer are follows: transfersomes > 3% dipentene > 3% azone > common nana-liposome.Conclusion It was showed that various transder enhancers could promote absorption of ketoconazole in different degrees and transfersomes was the most effective.
2005, (4): @-@.
Abstract:
Objective The results of serum concentration monitoring of 4 antiepileptic drugs with 357 measurements in 164 epilepsy children were evaluated in order to guide clinical rational administration of these antiepileptic drugs.Methods The serum concentration of antiepileptic drugs sodium valproate (VPA), carbamazepine (CBZ),sodium phenytoin (PHT) and phenobarbital (PB) were monitored by HPLC.Results The rate of serum concentrations which were in theraputic window is 47.1%,and which were lower and higher Than theraputic window are 45.9% and 7.0% respectively.The serum concentrations in normal range are founded in only 49.8% of all patients by routine administration, and deviate from normal range by 76.2% in combination with other drugs.Conclusion Serum concentration monitor is an important basis for the treatment of children's epilepsies with antiepileptic drugs, especially in combination with other drugs.
Objective The results of serum concentration monitoring of 4 antiepileptic drugs with 357 measurements in 164 epilepsy children were evaluated in order to guide clinical rational administration of these antiepileptic drugs.Methods The serum concentration of antiepileptic drugs sodium valproate (VPA), carbamazepine (CBZ),sodium phenytoin (PHT) and phenobarbital (PB) were monitored by HPLC.Results The rate of serum concentrations which were in theraputic window is 47.1%,and which were lower and higher Than theraputic window are 45.9% and 7.0% respectively.The serum concentrations in normal range are founded in only 49.8% of all patients by routine administration, and deviate from normal range by 76.2% in combination with other drugs.Conclusion Serum concentration monitor is an important basis for the treatment of children's epilepsies with antiepileptic drugs, especially in combination with other drugs.
2005, (4): @-@.
Abstract:
Objective To develop a RP-HPLC method for determination of baicalin in Zhenhuang Wan.Methods Alltima C18 column was used and methanol-water-acetic acid glacial (50 : 50 :1) was used as mobile phase,detection wavelegth was 273nm.Results There was a good linear relationship between the concentration of baicalin and absorption area value in range of 7.444 - 37.220μg r = 0.999 7.The average recovery was 98.55% with RSD=0.65% (n=5).Conclusion The method was repeatable and accurate.
Objective To develop a RP-HPLC method for determination of baicalin in Zhenhuang Wan.Methods Alltima C18 column was used and methanol-water-acetic acid glacial (50 : 50 :1) was used as mobile phase,detection wavelegth was 273nm.Results There was a good linear relationship between the concentration of baicalin and absorption area value in range of 7.444 - 37.220μg r = 0.999 7.The average recovery was 98.55% with RSD=0.65% (n=5).Conclusion The method was repeatable and accurate.
2005, (4): @-@.
Abstract:
Objective To establish a HPLC method for the determination of salicylic acid and phenol in salicylic acid and phenol lotion.Methods Shim-pack CLC-CN column was used, and the column temperature was maintained at 40℃.The mobile phase was consisted of methanol and water(28 : 72), and adjusted to pH 5.0 by glacial acetic acid.The flow rate was 0.9mL/min.The detection wavelength was 277nm.Results The linear range of standard curve of salicylic acid and phenol were between 10.0-500.0 μg/ mL(r=0.999 9) and between 10.0-200.0μg/mL(r= 0.999 9), respectively.The average recovery was 100.9% and 100.8%. Conclusion The method was simple, accurate.It can be used for the quality control of salicylic acid and phenol lotion.
Objective To establish a HPLC method for the determination of salicylic acid and phenol in salicylic acid and phenol lotion.Methods Shim-pack CLC-CN column was used, and the column temperature was maintained at 40℃.The mobile phase was consisted of methanol and water(28 : 72), and adjusted to pH 5.0 by glacial acetic acid.The flow rate was 0.9mL/min.The detection wavelength was 277nm.Results The linear range of standard curve of salicylic acid and phenol were between 10.0-500.0 μg/ mL(r=0.999 9) and between 10.0-200.0μg/mL(r= 0.999 9), respectively.The average recovery was 100.9% and 100.8%. Conclusion The method was simple, accurate.It can be used for the quality control of salicylic acid and phenol lotion.
2005, (4): @-228,250.
Abstract:
Objective To prepare and establish a method for determination of ipriflavone capsules. Methods Ipriflavone capsules were prepared by L-HPC and microcrystalline cellulose et al. It's content was determinated with the wavelength of 300. 4nm by spec-trophotometry.Results The content of ipriflavone and absorbance was good linear relation in the range of 3.95 - 15.79 μg/mL (r=0.9999), with average percent recovery 100.4% and RSD 1.2% (n=5). Conclusion The technological process is simple and quality is controllable.
Objective To prepare and establish a method for determination of ipriflavone capsules. Methods Ipriflavone capsules were prepared by L-HPC and microcrystalline cellulose et al. It's content was determinated with the wavelength of 300. 4nm by spec-trophotometry.Results The content of ipriflavone and absorbance was good linear relation in the range of 3.95 - 15.79 μg/mL (r=0.9999), with average percent recovery 100.4% and RSD 1.2% (n=5). Conclusion The technological process is simple and quality is controllable.
2005, (4): @-@.
Abstract:
Objective To develop the ability of emergency medical supply of the P.L.A.during emergencies. Method Analyze the problem of the Emergency Pharmaceutical Supply of the P.L.A.meeting in the period of SARS. Result: Put forward counter-measures and advice to develop and improve the Emergency Pharmaceutical Supply System of the P.R.C.Conclusion The development and improvement of the Emergency medical Supply System of the P.L.A has great significance to enhance the ability of the whole emergency-response systems of the P.L.A.
Objective To develop the ability of emergency medical supply of the P.L.A.during emergencies. Method Analyze the problem of the Emergency Pharmaceutical Supply of the P.L.A.meeting in the period of SARS. Result: Put forward counter-measures and advice to develop and improve the Emergency Pharmaceutical Supply System of the P.R.C.Conclusion The development and improvement of the Emergency medical Supply System of the P.L.A has great significance to enhance the ability of the whole emergency-response systems of the P.L.A.
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