2005 Vol. 23, No. 3
Display Method:
2005, (3): 148-150.
Abstract:
Objective To establish a near infrared diffuse reflectance spectroscopic (NIRDRS) method for the identification of Rheum officinale Baill from different production area.Method The near infrared spectra of Rheum officinale Baill from different origin and Rh:franzenbachii munt were treated respectively by cluster analysis provided by OPUS software and convolution transform-visualization-similarity (CVS) analysis developed by SMMU.Result Cluster analysis resulted in some confusion of the medicinal materials with each other, while the CVS analysis provided a better resolution.The similarity coefficients of the authentic and pseudo herbs were less than 0.68.The coefficients within the authentic herbs were greater than 0.81, while those within the same production area were greater than 0.92.Conclusion The simple and fast NIRDRS combined with CVS analysis provided a new approach to identify Rheum officinale Baill from different origin and Rh:franzenbachii munt which coincided quite well with the traditional morphological classification of the traditional Chinese medicines.
Objective To establish a near infrared diffuse reflectance spectroscopic (NIRDRS) method for the identification of Rheum officinale Baill from different production area.Method The near infrared spectra of Rheum officinale Baill from different origin and Rh:franzenbachii munt were treated respectively by cluster analysis provided by OPUS software and convolution transform-visualization-similarity (CVS) analysis developed by SMMU.Result Cluster analysis resulted in some confusion of the medicinal materials with each other, while the CVS analysis provided a better resolution.The similarity coefficients of the authentic and pseudo herbs were less than 0.68.The coefficients within the authentic herbs were greater than 0.81, while those within the same production area were greater than 0.92.Conclusion The simple and fast NIRDRS combined with CVS analysis provided a new approach to identify Rheum officinale Baill from different origin and Rh:franzenbachii munt which coincided quite well with the traditional morphological classification of the traditional Chinese medicines.
2005, (3): 150-154.
Abstract:
Objective To compare the microcapsules produced by single coacervation with that produced by complex coacervation, to sure which method was better.Methods Using ketoconazole as core, and the gelatin-acacia gum as the sealing material, microcapsules were produced by single coacervation and complex coacervation respectively.Their shape and diameter were observed under the microscope, and the concentration of ketoconazole was measured by UV spectrophotomelry, their microencapsulating rates were also determinated.Results Microcapsules were white powder either produced by single coacervation or complex one.The diameter and microencapsulating rate of microcapsules produced by single coacerva tion were 32.20μm and 56.11% respectively, while 7.99μm and 83.42% were detected among the microcapsules produced by complex coacervation.Conclusion When microcapsules are prepared by the phase separation-coacervation, the complex coacervation is better.
Objective To compare the microcapsules produced by single coacervation with that produced by complex coacervation, to sure which method was better.Methods Using ketoconazole as core, and the gelatin-acacia gum as the sealing material, microcapsules were produced by single coacervation and complex coacervation respectively.Their shape and diameter were observed under the microscope, and the concentration of ketoconazole was measured by UV spectrophotomelry, their microencapsulating rates were also determinated.Results Microcapsules were white powder either produced by single coacervation or complex one.The diameter and microencapsulating rate of microcapsules produced by single coacerva tion were 32.20μm and 56.11% respectively, while 7.99μm and 83.42% were detected among the microcapsules produced by complex coacervation.Conclusion When microcapsules are prepared by the phase separation-coacervation, the complex coacervation is better.
2005, (3): 154-156.
Abstract:
Objective To determine constituents of amino acids in the aerial parts of Broussonetia papyrifera.Methods The constituents composition of the ammo acid of the aerial parts of Broussonetia papyrifera were analysised by Hitachi 835-50 automatic amino acid analyzer (Hitachi 835-50) Results All the aerial parts of Broussonetia papyrifera contain at least 16 kinds of amino acid, the main amino acids are asparlate、glumatic acid、 arginine、valine、proline and lysine, and seven of them are essential amino acids for human body.The fruits, leaves, male inflorescences and the aggregate fruits of Broussonetia papyrifera contain 12.44、24.35、15.88、11.94 gram total Amino acids and 3.92、9.95、9.7、3.27gram essential amino acids in 100 gram of sample powder respectively.The content of amino acids of leaves and fruits of Broussonetia papyrifera varies with different growing stages.Conclusion The leaves and aggregate fruits of Broussonetia papyrifera contain rich amino acids, which reserved to be further exploited.
Objective To determine constituents of amino acids in the aerial parts of Broussonetia papyrifera.Methods The constituents composition of the ammo acid of the aerial parts of Broussonetia papyrifera were analysised by Hitachi 835-50 automatic amino acid analyzer (Hitachi 835-50) Results All the aerial parts of Broussonetia papyrifera contain at least 16 kinds of amino acid, the main amino acids are asparlate、glumatic acid、 arginine、valine、proline and lysine, and seven of them are essential amino acids for human body.The fruits, leaves, male inflorescences and the aggregate fruits of Broussonetia papyrifera contain 12.44、24.35、15.88、11.94 gram total Amino acids and 3.92、9.95、9.7、3.27gram essential amino acids in 100 gram of sample powder respectively.The content of amino acids of leaves and fruits of Broussonetia papyrifera varies with different growing stages.Conclusion The leaves and aggregate fruits of Broussonetia papyrifera contain rich amino acids, which reserved to be further exploited.
2005, (3): 156-157,175.
Abstract:
Objective To observe the effect of modafinil on locomotor activity and on CNS in mice.Methods Male mice were used as subjects.Total range of motion, average velocity and linearity were evaluated fay locomotion activity video analysis system.Results Total range of motion, average velocity and linearity were significantly improved after ip 120 mg/kg Modafinil compared to saline treatment.The effect was partial antagonized by sc 60 mg/kg promethamine.Conclusion Modafinil can stimulate CNS in mice which may involve 5-HT system.
Objective To observe the effect of modafinil on locomotor activity and on CNS in mice.Methods Male mice were used as subjects.Total range of motion, average velocity and linearity were evaluated fay locomotion activity video analysis system.Results Total range of motion, average velocity and linearity were significantly improved after ip 120 mg/kg Modafinil compared to saline treatment.The effect was partial antagonized by sc 60 mg/kg promethamine.Conclusion Modafinil can stimulate CNS in mice which may involve 5-HT system.
2005, (3): 163-165.
Abstract:
Objective To determine the content of hyperoside and oleanolic acid in Diospyros kaki.Methods The HPLC method was used to determine the content of hyperoside and oleanolic acid in Diospyros kaki.The analysis of hyperoside was carried out on Shi madzu C18column (150mm×4.6mm,5μm).The mobile phase was MeOH-0.2%H3PO4(45:55).The flow-rate was 1.0mL/min.The detection wave-length was 350nm.The analysis of oleanolic acid was carried out on ShimadzuC18column(150mm×4.6mm,5μm).The mobile phase was MeOH-0.2% H3PO4(90:10).The flow -rate was 0.8 mL/min.The detection wave -length was 220nm.Results The liner range of hyperoside was 0.50-2.50μg and gave a correlation (r) of 0.9999.The recovery was 99.65% and RSD was 1.24 % .The liner range of oleanolic acid was1.56-5.20μg and gave a correlation (r) of 0.9990.The recovery was 97.02% and RSD was 2.84 % .Conclusion The method is simple, fast and had a good linear relationship.It is to determine the content of hyperoside and oleanolic acid in Diospyros kaki.
Objective To determine the content of hyperoside and oleanolic acid in Diospyros kaki.Methods The HPLC method was used to determine the content of hyperoside and oleanolic acid in Diospyros kaki.The analysis of hyperoside was carried out on Shi madzu C18column (150mm×4.6mm,5μm).The mobile phase was MeOH-0.2%H3PO4(45:55).The flow-rate was 1.0mL/min.The detection wave-length was 350nm.The analysis of oleanolic acid was carried out on ShimadzuC18column(150mm×4.6mm,5μm).The mobile phase was MeOH-0.2% H3PO4(90:10).The flow -rate was 0.8 mL/min.The detection wave -length was 220nm.Results The liner range of hyperoside was 0.50-2.50μg and gave a correlation (r) of 0.9999.The recovery was 99.65% and RSD was 1.24 % .The liner range of oleanolic acid was1.56-5.20μg and gave a correlation (r) of 0.9990.The recovery was 97.02% and RSD was 2.84 % .Conclusion The method is simple, fast and had a good linear relationship.It is to determine the content of hyperoside and oleanolic acid in Diospyros kaki.
2005, (3): 165-167.
Abstract:
Objiective:To establish a HPLC method for determination of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in Fu fang Shouwu Buye.Method:A hypersil ODS column with a mobile phase composed of acetonitrile-water(30:70) was used.The detection wavelength was 320nm.Results The linear response range of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside was 25-400mg/mL(r=0.999 5).The mean recovery was 99.4%.Conclusion The method is simple,rapid and accurate.
Objiective:To establish a HPLC method for determination of 2,3,5,4′-tetrahydroxystilbene-2-O-β-D-glucoside in Fu fang Shouwu Buye.Method:A hypersil ODS column with a mobile phase composed of acetonitrile-water(30:70) was used.The detection wavelength was 320nm.Results The linear response range of 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside was 25-400mg/mL(r=0.999 5).The mean recovery was 99.4%.Conclusion The method is simple,rapid and accurate.
2005, (3): 167-169.
Abstract:
Objective To establish a quantitative analysis method for camphor and dexamethason acetate in compound dexametha son acetate liniment.Methods The camphor was determined directly at 305nm by first derivative spectrometry.The dexamethason acetate was determined at 485nm by seeable spectrometry.Results The calibra-tion curve of camphor was linear in the range from 1.62-3.22mg/mL with r=0.999 4,the average recovery and relative standard deviation were 99.89% and 0.34% respectively.The calibration curve of dexamethason acetate was linear in the range from 122.04-227.81μg/mL with r= 0.9997, the average recovery and RSD were 99.18% and 1.32% respectively.Conclusions The method is simple, rapid and accurate.It can be used to control the quality of compound dexame-thason acetate liniment.
Objective To establish a quantitative analysis method for camphor and dexamethason acetate in compound dexametha son acetate liniment.Methods The camphor was determined directly at 305nm by first derivative spectrometry.The dexamethason acetate was determined at 485nm by seeable spectrometry.Results The calibra-tion curve of camphor was linear in the range from 1.62-3.22mg/mL with r=0.999 4,the average recovery and relative standard deviation were 99.89% and 0.34% respectively.The calibration curve of dexamethason acetate was linear in the range from 122.04-227.81μg/mL with r= 0.9997, the average recovery and RSD were 99.18% and 1.32% respectively.Conclusions The method is simple, rapid and accurate.It can be used to control the quality of compound dexame-thason acetate liniment.
2005, (3): 169-171.
Abstract:
Objective To establish a RP-HPLC method for determinate palonosetron in human hepatic microsomes.Methods Pal onosetron in human hepatic microsomal incubates was assayed on a Nova-park C18(5μm, 200×4.6mm) column with a mobile phase of methanol-0.01mol/L KH2PO4 (80:20, v/v) at a flow-rate of 1.0 mL/min.A UV-VIS detector was operated at 240nm.Results The assay was linear from 5-100 μmol/L for palonosetron (r= 0.999 8 ).The limit of detection was 0.2 μmol/L ( signal to noise ratio≥3) and the limit of quantification was 1.0μmol/L (RSD =4.88%,n=3).The method afforded recoveries of 96.0%-103.0% (n=5), and inter-day and intra-day variation coefficient were <7% and 10% (n=5).Conclusion The method is simple, accurate and can be used to study the metabolism of palonosetron in human hepatic microsomes.
Objective To establish a RP-HPLC method for determinate palonosetron in human hepatic microsomes.Methods Pal onosetron in human hepatic microsomal incubates was assayed on a Nova-park C18(5μm, 200×4.6mm) column with a mobile phase of methanol-0.01mol/L KH2PO4 (80:20, v/v) at a flow-rate of 1.0 mL/min.A UV-VIS detector was operated at 240nm.Results The assay was linear from 5-100 μmol/L for palonosetron (r= 0.999 8 ).The limit of detection was 0.2 μmol/L ( signal to noise ratio≥3) and the limit of quantification was 1.0μmol/L (RSD =4.88%,n=3).The method afforded recoveries of 96.0%-103.0% (n=5), and inter-day and intra-day variation coefficient were <7% and 10% (n=5).Conclusion The method is simple, accurate and can be used to study the metabolism of palonosetron in human hepatic microsomes.
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