基于GC-MS技术的金钱草干预结晶肾损伤小鼠血清代谢组学研究

杜毅; 丁德英; 吕磊; 李玲

doi:10.3969/j.issn.1006-0111.2019.04.009

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

基于GC-MS技术的金钱草干预结晶肾损伤小鼠血清代谢组学研究

杜毅, 丁德英, 吕磊, 李玲

文章导航 > 药学实践与服务 > 2019 > 37(4): 332-336

杜毅, 丁德英, 吕磊, 李玲. 基于GC-MS技术的金钱草干预结晶肾损伤小鼠血清代谢组学研究[J]. 药学实践与服务, 2019, 37(4): 332-336. doi: 10.3969/j.issn.1006-0111.2019.04.009

引用本文:

DU Yi, DING Deying, LÜ Lei, LI Ling. Metabolomics study of Lysimachiae Herba on mice with crystallization kidney injury by GC-MS[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 332-336. doi: 10.3969/j.issn.1006-0111.2019.04.009

Citation:

DU Yi, DING Deying, LÜ Lei, LI Ling. Metabolomics study of Lysimachiae Herba on mice with crystallization kidney injury by GC-MS[J]. Journal of Pharmaceutical Practice and Service, 2019, 37(4): 332-336. doi: 10.3969/j.issn.1006-0111.2019.04.009

基于GC-MS技术的金钱草干预结晶肾损伤小鼠血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2019.04.009

杜毅¹,
丁德英²,
吕磊³,
李玲^2,

1.
上海交通大学医学院附属新华医院药学部, 上海 200092
2.
海军军医大学药学院分析测试中心, 上海 200433
3.
上海东方肝胆外科医院药材科, 上海 200438

Metabolomics study of Lysimachiae Herba on mice with crystallization kidney injury by GC-MS

1.
Department of Pharmacy, Xinhua Hospital Affiliated to School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China
2.
Pharmaceutical Analysis Center, School of Pharmacy, Naval Medical University, Shanghai 200433, China
3.
Department of Pharmacy, Shanghai Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

摘要: 目的利用血清代谢组学方法监测结晶肾损伤相关的代谢变化，评价金钱草提取物治疗结晶肾损伤的作用。方法将21只野生型C57BL/6小鼠随机分为3组：空白对照组、结晶肾损伤模型组和金钱草干预组。采用乙醛酸盐诱导小鼠结晶肾损伤模型，给予6 d相应的治疗后，取小鼠血清进行气相色谱-质谱联用仪分析，结合模式识别筛选模型组和对照组之间的差异代谢物，并以此评价金钱草的治疗作用。结果血清代谢组学分析鉴定了14种结晶肾损伤相关的生物标志物，包括嘌呤代谢物、氨基酸代谢物、脂肪酸代谢物等，同时数据显示金钱草提取物对结晶肾损伤有改善作用。结论金钱草提取物能够通过调节失衡的嘌呤代谢、氨基酸代谢、糖代谢等途径而发挥预防和治疗结晶肾损伤作用。
- 结晶肾损伤 /
- 金钱草 /
- 气相色谱-质谱联用 /
- 代谢组学
Abstract: Objective To evaluate therapeutic effects of Lysimachiae Herba for crystallization kidney injury with serum metabolomics method. Methods 21 rats were randomly divided into three groups including control group,crystallization kidney injury model group and Lysimachiae Herba-treated group.The medicated group received Lysimachiae Herba for 6 days.Then,the serum samples of each rat were collected and analyzed by Gas Chromatography-Mass Spectrometer (GC-MS).The pattern recognition analysis of metabolomics differences among three groups was used to evaluate therapeutic effects of Lysimachiae Herba. Results 14 metabolites were identified through serum metabolomics analysis.The possible mechanism of crystallization kidney injury was mainly related to the metabolic products of purine,amino acid,fatty acid and sugar.The kidney injury by crystallization was alleviated by Lysimachiae Herba. Conclusion Lysimachiae Herba has preventive and therapeutic effects for crystallization kidney injury through partially regulating the perturbed purine metabolism,amino acid metabolism and glycometabolism.
- kidney injury by crystallization /
- Lysimachiae Herba /
- GC-MS /
- metabolomics

[1]	简宇,马进.慢性肾衰竭中西医治疗进展[J].辽宁中医药大学学报,2014,16(11):198-201.
[2]	李昕.慢性肾功能衰竭并发症的研究进展[J].医学信息:中旬刊,2010,5(5):1334.
[3]	李飞艳,周日宝,刘群群.金钱草及其混伪品的微性状鉴定[J].海峡药学,2018,30(7):39-41.
[4]	武维波,刘永,刘桂营.利尿排石汤治疗泌尿系结石780例[J].实用中医药杂志,2013,29(7):559.
[5]	赵建一.金钱草治疗尿结石的机理作用研究[J].中医临床研究,2013,5(16):37-38.
[6]	GUO L,TAN G G,LIU P,et al.Three plasma metabolite signatures for diagnosing high altitude pulmonary edema[J].Sci Rep,2015,5:15126.
[7]	BIJLSMA S,BOBELDIJK I,VERHEIJ E R,et al.Large-scale human metabolomics studies:A strategy for data (pre-) processing and validation[J].Anal Chem,2006,78(2):567-574.
[8]	EDVARDSSON V O,INDRIDASON O S,HARALDSSON G,et al.Temporal trends in the incidence of kidney stone disease[J].Kidney Int,2013,83(1):146-152.
[9]	彭明,顾向明,杜国有,等.泌尿系结石患者血清胱抑素C的含量变化分析[J].检验医学与临床,2011,8(2):172-173.
[10]	ZAKARIA F A.Effect of oxygen derived free radicals and glycine on sodium-potassium adenosine triphosphatase in the basolateral membrane of the kidney in ischemia-reperfusion[J].Saudi Med J,2002,23(11):1380-1385.
[11]	GARIBOTTO G,BONANNI A,VERZOLA D.Effect of kidney failure and hemodialysis on protein and amino acid metabolism[J].Curr Opin Clin Nutr Metab Care,2012,15(1):78-84.
[12]	张珺,尹磊,王昭君,等.脓毒症相关性肾损伤临床特征及预后分析[J].宁夏医学杂志,2018,40(9):807-809.

[1]	夏哲炜, 曾垣烨, 朱海菲, 李育, 陈啸飞. 核磁共振磷谱法测定磷酸氢钙咀嚼片中药物含量 . 药学实践与服务, 2024, 42(9): 399-401, 406. doi: 10.12206/j.issn.2097-2024.202404063
[2]	杨嘉宁, 赵一颖, 肖伟. 七味脂肝方对非酒精性脂肪性肝炎动物模型的药效学评价 . 药学实践与服务, 2024, 42(9): 389-398. doi: 10.12206/j.issn.2097-2024.202404096
[3]	宋雨桐, 夏德润, 顾珩, 唐少文, 易洪刚, 沃红梅. 帕博利珠单抗与铂类化疗方案在晚期非小细胞肺癌一线治疗中的药物经济学评价 . 药学实践与服务, 2024, 42(8): 334-340. doi: 10.12206/j.issn.2097-2024.202303023
[4]	张艺昕, 关欣怡, 王博宁, 闻俊, 洪战英. 二氢吡啶类钙离子拮抗药物手性分析及其立体选择性药动学研究进展 . 药学实践与服务, 2024, 42(8): 319-324. doi: 10.12206/j.issn.2097-2024.202308062
[5]	崔亚玲, 吴琼, 马良煜, 胡北, 姚东, 许子华. 肝素钠肌醇烟酸酯乳膏中肌醇烟酸酯皮肤药动学研究 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202404006
[6]	毛智毅, 王筱燕, 陈晓颖, 汤逸斐. 度拉糖肽联合二甲双胍对肥胖型2型糖尿病患者机体代谢、体脂成分及血清脂肪因子的影响 . 药学实践与服务, 2024, 42(7): 305-309. doi: 10.12206/j.issn.2097-2024.202305032
[7]	赖立勇, 夏天爽, 徐圣焱, 蒋益萍, 岳小强, 辛海量. 中药青蒿抗氧化活性的谱效关系研究 . 药学实践与服务, 2024, 42(5): 203-210, 216. doi: 10.12206/j.issn.2097-2024.202211012
[8]	姜涛, 徐卫凡, 蒋益萍, 夏天爽, 辛海量. 巴戟天丸组方对Aβ损伤成骨细胞的作用及基于网络药理学的机制研究 . 药学实践与服务, 2024, 42(7): 285-290, 296. doi: 10.12206/j.issn.2097-2024.202305011
[9]	李想, 陆鸿远, 张明玉, 高欢, 姚东, 许子华. 米格列醇激活UCP1介导棕色脂肪对冷暴露小鼠损伤的研究 . 药学实践与服务, 2024, 42(): 1-6. doi: 10.12206/j.issn.2097-2024.202404005
[10]	何静, 安晔, 张朝绅. 复方黑参滴丸与复方黑参丸药效学实验比较研究 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202404009
[11]	张岩, 李炎君, 刘家荟, 邓娇, 原苑, 张敬一. 药物性肝损伤不良反应分析 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202404034
[12]	孙丹倪, 黄勇, 张嘉宝, 王培. 代谢相关脂肪性肝病的无创诊断与药物治疗 . 药学实践与服务, 2024, 42(10): 411-418. doi: 10.12206/j.issn.2097-2024.202403049
[13]	张成中, 朱雪艳, 卜其涛, 王宏瑞, 黄宝康. 基于网络药理学与分子对接预测鸡骨草特征图谱研究 . 药学实践与服务, 2024, 42(8): 350-358. doi: 10.12206/j.issn.2097-2024.202303048
[14]	迟文雅, 袁艳, 李伟林, 吴茼妤, 俞媛. 负载骨髓间充质干细胞/白藜芦醇脂质体的水凝胶支架用于创伤性脑损伤治疗 . 药学实践与服务, 2024, 42(): 1-8. doi: 10.12206/j.issn.2097-2024.202406034

点击查看大图

计量

文章访问数: 3338
HTML全文浏览量: 434
PDF下载量: 328
被引次数: 0

基于GC-MS技术的金钱草干预结晶肾损伤小鼠血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2019.04.009

1. 上海交通大学医学院附属新华医院药学部, 上海 200092

2. 海军军医大学药学院分析测试中心, 上海 200433

3. 上海东方肝胆外科医院药材科, 上海 200438

收稿日期: 2018-12-13
修回日期: 2019-05-07

关键词:

结晶肾损伤 /

金钱草 /

气相色谱-质谱联用 /

代谢组学

摘要: 目的利用血清代谢组学方法监测结晶肾损伤相关的代谢变化，评价金钱草提取物治疗结晶肾损伤的作用。方法将21只野生型C57BL/6小鼠随机分为3组：空白对照组、结晶肾损伤模型组和金钱草干预组。采用乙醛酸盐诱导小鼠结晶肾损伤模型，给予6 d相应的治疗后，取小鼠血清进行气相色谱-质谱联用仪分析，结合模式识别筛选模型组和对照组之间的差异代谢物，并以此评价金钱草的治疗作用。结果血清代谢组学分析鉴定了14种结晶肾损伤相关的生物标志物，包括嘌呤代谢物、氨基酸代谢物、脂肪酸代谢物等，同时数据显示金钱草提取物对结晶肾损伤有改善作用。结论金钱草提取物能够通过调节失衡的嘌呤代谢、氨基酸代谢、糖代谢等途径而发挥预防和治疗结晶肾损伤作用。

English Abstract

Metabolomics study of Lysimachiae Herba on mice with crystallization kidney injury by GC-MS

1. Department of Pharmacy, Xinhua Hospital Affiliated to School of Medicine, Shanghai Jiaotong University, Shanghai 200092, China

2. Pharmaceutical Analysis Center, School of Pharmacy, Naval Medical University, Shanghai 200433, China

3. Department of Pharmacy, Shanghai Eastern Hepatobiliary Surgery Hospital, Shanghai 200438, China

Received Date: 2018-12-13
Rev Recd Date: 2019-05-07

Keywords:

Abstract: Objective To evaluate therapeutic effects of Lysimachiae Herba for crystallization kidney injury with serum metabolomics method. Methods 21 rats were randomly divided into three groups including control group,crystallization kidney injury model group and Lysimachiae Herba-treated group.The medicated group received Lysimachiae Herba for 6 days.Then,the serum samples of each rat were collected and analyzed by Gas Chromatography-Mass Spectrometer (GC-MS).The pattern recognition analysis of metabolomics differences among three groups was used to evaluate therapeutic effects of Lysimachiae Herba. Results 14 metabolites were identified through serum metabolomics analysis.The possible mechanism of crystallization kidney injury was mainly related to the metabolic products of purine,amino acid,fatty acid and sugar.The kidney injury by crystallization was alleviated by Lysimachiae Herba. Conclusion Lysimachiae Herba has preventive and therapeutic effects for crystallization kidney injury through partially regulating the perturbed purine metabolism,amino acid metabolism and glycometabolism.

引用本文:

Citation:

全文HTML

参考文献 (12)

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

电话: （021）81871324,81871397 E-mail: [email protected]

网站备案号沪ICP备05003363号-1

本系统由北京仁和汇智信息技术有限公司开发

留言板

基于GC-MS技术的金钱草干预结晶肾损伤小鼠血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2019.04.009

Metabolomics study of Lysimachiae Herba on mice with crystallization kidney injury by GC-MS

计量

出版历程

基于GC-MS技术的金钱草干预结晶肾损伤小鼠血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2019.04.009

1. 上海交通大学医学院附属新华医院药学部, 上海 200092 2. 海军军医大学药学院分析测试中心, 上海 200433 3. 上海东方肝胆外科医院药材科, 上海 200438

English Abstract

Metabolomics study of Lysimachiae Herba on mice with crystallization kidney injury by GC-MS

全文HTML

目录

1. 上海交通大学医学院附属新华医院药学部, 上海 200092

2. 海军军医大学药学院分析测试中心, 上海 200433

3. 上海东方肝胆外科医院药材科, 上海 200438