Chemical constituents and cytotoxic activities in breast cancer of Euphorbia ebracteolata
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摘要: 目的 研究大戟科植物月腺大戟的化学成分及其对乳腺癌细胞毒活性。 方法 采用多种色谱技术对化合物进行分离纯化，根据化合物的理化性质和光谱数据分析鉴定化合物的结构。采用噻唑蓝（MTT）比色法研究化合物对6种乳腺癌细胞的抑制活性。 结果 月腺大戟植物中分离得到10个化合物，分别鉴定为：2，4-二羟基-6-甲氧基-3-甲酰基苯乙酮（ 1 ）；2，4-二羟基-6-甲氧基-3-甲基苯乙酮（ 2 ）；月腺大戟素A（ 3 ）；3，3'-乙酰基-4，4'-二甲氧基-2，2'，6，6'-四羟基二苯基甲烷（ 4 ）；岩大戟内酯B（ 5 ）；岩大戟内酯A（ 6 ）；亚油酸（ 7 ）；fischeria A（ 8 ）；β-香树脂醇乙酸酯（ 9 ）；单十九酸甘油酯（ 10 ）。乳腺癌细胞毒活性研究显示：化合物3对乳腺癌细胞MDA-MB-231、Sum149、MCF7、ZR-75-1、SKBr3、BT474的IC50值分别为6.69、5.50、5.50、7.08、8.64、5.42 μmol/L。 结论 化合物6、7、8、10为首次从该植物中分离得到；化合物3对乳腺癌细胞具有显著的抑制活性。Abstract: Objective To investigate the chemical constituents and cytotoxic activities of breast cancer in the roots of Euphorbia ebracteolata. Methods The chemical constituents were isolated and purified by various chromatographic techniques.The in vitro cytotoxic activities of breast cancer of the isolated compounds were studied by MTT method.Their structures were identified on the basis of spectroscopic analyses and physiochemical properties. Results Ten compounds were isolated from the roots of Euphorbia ebracteolata,and elucidated as 2,4-dihydroxy-6-methoxy-3-form yl-1-acetophenon ( 1 ),2,4-dihydroxy-6-methoxy- 3-methyl-1- acetophenon ( 2 ),ebracteolatain A ( 3 ),3,3'-diacetyl-4,4'-dimethoxy-2,2',6,6'-tetrahydroxy diphenylmethan ( 4 ),jolkinolide B ( 5 ),jolkinolide A ( 6 ),linoleic acid ( 7 ),fischeria A ( 8 ),β-amyrin acetate ( 9 ),nonadecanoic acid monoglyceride ( 10 ).Compound 3 showed cytotoxicity against MDA-MB-231 cell、Sum149 cell、MCF7 cell、ZR-75-1 cell、SKBr3 cell、BT474 cell with IC50values of (6.69±0.128),(5.50±0.126),(5.50±0.126),(7.08±0.111),(8.64±0.197),(5.42±0.113)μmol/L,respectively.Compound 4 had no cytotoxicity on the above cancer cells. Conclusion Compounds 6,7,8,10 were firstly isolated from Euphorbia ebracteolata.Compound 3 showed significant cytotoxicity on breast cancer cells.
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