Study on hydrophilic chemical constituents from Thesium Chinensis Turcz
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摘要: 目的 对百蕊草中亲水性化学成分进行研究。 方法 采用硅胶柱色谱、SephadexLH-20凝胶柱色谱等分离手段，对百蕊草水提取物经AB-8大孔树脂吸附，50%乙醇洗脱的亲水性组分（TT50）进行分离纯化，应用核磁共振波谱分析、结合文献报道鉴定化合物结构。 结果 从TT50中分离纯化得到6个化合物，分别鉴定为：山奈酚（ 1 ），紫云英苷（ 2 ），山奈酚-3，7-二-O-β-D-吡喃葡萄糖苷（ 3 ），山奈酚-3-O-L-吡喃鼠李糖基（1→2）-β-D-吡喃葡萄糖苷（ 4 ），山奈酚-3-O-L-吡喃鼠李糖基（1→2）-［6-O-乙酰基］-β-D-吡喃葡萄糖苷（ 5 ），芸香苷（ 6 ）。 结论 TT50中主要成分为以山奈酚为母核的黄酮苷类化合物，化合物 4 、 5 为首次从百蕊草中分离得到。Abstract: Objective To investigate the hydrophilic chemical constituents from Thesium Chinensis Turcz. Methods The water extract of Thesium Chinensis Turcz. was adsorbed onto AB-8 resin column and eluted with 50% ethanol to give the hydrophilic portion TT50. TT50 was further separated and purified by repeated column chromatography on silica gel,Sephadex LH-20. The structures of these purified compounds were identified by NMR spectral analysis and comparison with the reported data. Results Six compounds were isolated and identified as kaempferol ( 1 ),kaempferol-3-O-glucoside ( 2 ),kaepmferol-3,7-di-O-β-D-glucopyranoside ( 3 ),kaempferol-3-O-L-rhamnopyranosyl(1→2)-β-D-glucopyranoside ( 4 ),kaemperol-3-O-α-L-rhamnopyranosyl(1→2)-[6-O-acetyl]-β-D-glucopyranoside ( 5 ),rutinoside ( 6 ). Conclusion The main constituents of TT50 were kaempferol glycosides. Compounds 4 and 5 were isolated from this plant for the first time.
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Key words:
- Thesium chinensis Turcz /
- chemical constituents /
- flavonoid glycosides
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