Simultaneous determination of dexamethasone acetate, camphor and phenol in compound cream by HPLC method
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摘要: 目的 建立以HPLC法同时测定地米樟酚乳膏中醋酸地塞米松、樟脑和苯酚含量的方法。 方法 色谱柱为SHIADZU-GL Inertsil® ODS-3 RP C18，流动相为甲醇-水（60：40），检测波长为285 nm，流速为1.0 ml/min，柱温为40℃，外标法定量。 结果 醋酸地塞米松、樟脑和苯酚的线性范围分别为4.024~40.24、101.7~2 033和10.38~425.2 μg/ml，相关系数r >0.999 5；三者的平均回收率分别为101.2%（RSD为0.56%）、99.89%（RSD为0.72%）、100.2%（RSD为0.97%）；重复性试验中RSD均小于1.5%。 结论 本方法简便、准确，重复性好，可用于地米樟酚乳膏的质量控制。Abstract: Objective To establish a HPLC method for simultaneously determining the content of dexamethasone acetate, camphor and phenol in compound cream. Methods The separation was performed on a SHIADZU-GL Inertsil® ODS-3 RP C18 analytical column with the mobile phase consisting of methanol-water (60:40). The flow rate was 1.0 ml/min. The detection wave length was 285 nm and the column temperature was 40℃. Results Dexamethasone acetate,camphor and phenol showed good linearity (r>0.999 5, n=7) within the concentration range of 4.024-40.24,101.7-2 033 and 10.38-425.2 μg/ml,respectively. The average recovery of dexamethasone acetate, camphor and phenol was 101.2%(RSD was 0.56%),99.89%(RSD was 0.72%),100.2%(RSD was 0.97%),respectively. Moreover, the RSDs were less than 1.5% in the repeated tests. Conclusion The method was simple, quick and accurate. It is suitable for the quality control of dexamethasone acetate camphor and phenol cream.
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Key words:
- HPLC /
- cream /
- dexamethasone acetate /
- camphor /
- phenol /
- determination
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[1] 吴洪文,吴敏.RP-HPLC法测定复方鼻用软膏中醋酸地塞米松含量[J].中国药师,2004,7(7):529-530. [2] 李冬,高红英.采用HPLC法测定醋酸地塞米松片的含量[J].中小企业管理与科技,2008(8):174-175. [3] 陈江飞,苗彩云.HPLC法测定复方醋酸地塞米松搽剂中醋酸地塞米松和樟脑的含量[J].中国临床药学杂志,2010,19(2):108-111. [4] 国家药典委员会. 中华人民共和国药典2015年版二部[S].北京:中国医药科技出版社,2015:1531. [5] 张利平,林伟萍,陈健苗,等.高效液相色谱法测定氯霉素地塞米松乳膏中两组分含量[J].中国药业,2008,17(15):26-27. [6] 陆松伟,宋洪杰,陈方剑.HPLC法同时测定复方樟脑搽剂中苯酚和樟脑含量[J].药学实践杂志,2011,29(5):347-349. -
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