Content variation of four flavonoid glycosides in Hippophae rhamnoides L. leaves before and after fermentation assayed by RP-HPLC
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摘要: 目的 建立反向高效液相色谱（RP-HPLC）法比较沙棘叶发酵前后芦丁、异槲皮苷、水仙苷、异鼠李素-3-O-葡萄糖苷含量的变化。 方法 沙棘叶及其发酵茶水提液浓缩干燥后用70%乙醇溶解，分析采用HPLC法，Extend-C18色谱柱（4.6 mm×250 mm，5 μm），流动相为乙腈-0.1%甲酸水溶液，流速1 ml/min，检测波长为356 nm，柱温30℃。 结果 沙棘叶中芦丁含量较高。经过发酵后，除异槲皮苷含量略有升高外，异鼠李素-3-O-葡萄糖苷含量变化不大，芦丁、水仙苷含量均有所下降。上述4种成分在一定范围内呈现良好的线性关系，相关系数r均大于0.9997；精密度、重复性和稳定性良好；加样回收率在96%~103%之间。 结论 沙棘叶发酵降低了芦丁和水仙苷含量，增加了异槲皮苷含量。该法简便、可靠，可为沙棘叶和沙棘叶发酵茶的质量评价提供科学依据。Abstract: Objective To compare the changes of four flavonoid glycosides in the leaves of Hippophae rhamnoides L. before and after fermentation. Methods The water extract of Hippophae rhamnoides L. leaves and its fermented tea were concentrated and desiccated. The dry extracts were dissolved in 70% ethanol. The chromatographic separation was performed with RP-HPLC method on an Extend-C18 column (4.6 mm×250 mm, 5 μm). Acetonitrile-0.1% formic acid was selected as mobile phase at the flow rate of 1.0 ml/min. The detection wavelength was 356 nm and the column temperature was 30℃. Results The rutin content was high in the leaves of Hippophae rhamnoides L. After fermentation, isoquercitrin content was increased, while the contents of rutin and narcissoside were reduced and isorhamnetin-3-O-glucoside stayed unchanged. There was a good linear relationship between the concentration and peak areas of the four compounds (r>0.9997). The average recoveries were between 96%-103%. Conclusion This established method is rapid and reliable, which can be used for the quality control of Hippophae rhamnoides L. leaves and its fermented tea.
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Key words:
- RP-HPLC /
- Hippophae rhamnoides L. leave /
- fermentation /
- flavonoid glycosides
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