转录因子<em>Cup2</em>对白念珠菌铜离子代谢、氧化应激调控作用的初步研究

张金宇; 王丽红; 秦玉璘; 张璐璐; 姜远英; 曹永兵

doi:10.3969/j.issn.1006-0111.2017.03.008

转录因子Cup2对白念珠菌铜离子代谢、氧化应激调控作用的初步研究

doi: 10.3969/j.issn.1006-0111.2017.03.008

1.
第二军医大学药学院新药研究中心, 上海 200433
2.
中国药科大学, 江苏南京 211198

基金项目: 国家自然科学基金资助项目（81673478）

Regulation and control of transcription factor Cup2 on Cu²⁺ metabolism and oxidative stress in Candida albicans

1.
Research and Develop Center of New Drug, School of Pharmacy; Second Military Medical University, Shanghai 200433, China
2.
Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China

摘要: 目的利用白念珠菌（Candida albicans）不同转录因子基因缺失菌，考察参与调控铜离子（Cu²⁺）代谢和氧化应激的重要转录因子。方法点板实验（spot assay）、生长曲线法。结果 spot assay筛选发现，转录因子Cup2缺失菌Cup2Δ/Δ对Cu²⁺的敏感性增加，进一步研究表明Cup2Δ/Δ在含5 mmol/L的Cu²⁺培养液中生长缓慢；Cup2Δ/Δ对H₂O₂的敏感性也有所增加，而且Cu²⁺协同H₂O₂发挥抑菌作用，在BCS螯合Cu²⁺后，增加了Cup2Δ/Δ和亲本菌SN250对H₂O₂诱导的氧化应激的耐受性。在氟康唑、咪康唑和酮康唑敏感性实验中，Cup2Δ/Δ并未表现出对唑类药物敏感。结论敲除转录因子Cup2，可增加白念珠菌对Cu²⁺和H₂O₂的敏感性。转录因子Cup2可能参与调控白念珠菌对Cu²⁺的代谢和H₂O₂诱导的氧化应激反应，但并未参与对唑类药物耐药性的调控。
- 白念珠菌 /
- 转录因子Cup2 /
- 铜离子(Cu²⁺) /
- 过氧化氢 /
- 药物敏感性
Abstract: Objective To detect the transcription factors of copper ion (Cu²⁺) metabolism and oxidative stress by Candida albicans knocked down different transcription factors. Methods Spot assay, growth curve were used. Results The sensitivity to Cu²⁺ in Cup2Δ/Δ was increasing and the growth of Cup2Δ/Δ was inhibited in 5 mmol /L Cu²⁺ medium. The results showed that Cup2Δ/Δ also increased the sensitivity to H₂O₂, interestingly, Cu²⁺and H₂O₂ played a synergistic antifungal effect.The tolerance of Cup2Δ/Δ and SN250 to H₂O₂ induced oxidative stress was increased after BCS chelating Cu²⁺. In the fluconazole, miconazole and ketoconazole susceptibility experiments, Cup2Δ/Δ did not show susceptibility to azole drugs. Conclusion Knockout transcription factor Cup2, which could increase the sensitivity to Cu²⁺ and H₂O₂in Candida albicans. Transcription factor Cup2 might be involved in the regulation and control of Candida albicansmetabolism on Cu²⁺ and oxidative stress induced by H₂O₂, but not involved in the regulation and control of drug resistance to azole drugs.
- Candida albicans /
- transcription factor Cup2 /
- copper ion (Cu²⁺) /
- hydrogen peroxide(H₂O₂) /
- drug susceptibility

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转录因子Cup2对白念珠菌铜离子代谢、氧化应激调控作用的初步研究

doi: 10.3969/j.issn.1006-0111.2017.03.008

Regulation and control of transcription factor Cup2 on Cu²⁺ metabolism and oxidative stress in Candida albicans

计量

转录因子Cup2对白念珠菌铜离子代谢、氧化应激调控作用的初步研究

doi: 10.3969/j.issn.1006-0111.2017.03.008

1. 第二军医大学药学院新药研究中心, 上海 200433

2. 中国药科大学, 江苏南京 211198

English Abstract

Regulation and control of transcription factor Cup2 on Cu²⁺ metabolism and oxidative stress in Candida albicans

1. Research and Develop Center of New Drug, School of Pharmacy; Second Military Medical University, Shanghai 200433, China

2. Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China

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转录因子Cup2对白念珠菌铜离子代谢、氧化应激调控作用的初步研究

doi: 10.3969/j.issn.1006-0111.2017.03.008

Regulation and control of transcription factor Cup2 on Cu2+ metabolism and oxidative stress in Candida albicans

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出版历程

转录因子Cup2对白念珠菌铜离子代谢、氧化应激调控作用的初步研究

doi: 10.3969/j.issn.1006-0111.2017.03.008

1. 第二军医大学药学院新药研究中心, 上海 200433 2. 中国药科大学, 江苏南京 211198

English Abstract

Regulation and control of transcription factor Cup2 on Cu2+ metabolism and oxidative stress in Candida albicans

1. Research and Develop Center of New Drug, School of Pharmacy; Second Military Medical University, Shanghai 200433, China 2. Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China

全文HTML

目录

Regulation and control of transcription factor Cup2 on Cu²⁺ metabolism and oxidative stress in Candida albicans

1. 第二军医大学药学院新药研究中心, 上海 200433

2. 中国药科大学, 江苏南京 211198

Regulation and control of transcription factor Cup2 on Cu²⁺ metabolism and oxidative stress in Candida albicans

1. Research and Develop Center of New Drug, School of Pharmacy; Second Military Medical University, Shanghai 200433, China

2. Department of Pharmacology, School of Pharmacy, China Pharmaceutical University, Nanjing 211198, China