摘要:
目的 建立人血清中丙戊酸浓度测定方法。 方法 将血清酸化,用乙醚提取,以环己烷羧酸为内标,α-溴苯乙酮为衍生化试剂,采用高效液相色谱法测定。色谱柱:Eclipse Plus C18柱(150 mm×4.6 mm,5 μm),流动相:甲醇-水(70:30),检测波长:248 nm,流速:1.0 ml/min。 结果 血清中丙戊酸浓度线性范围为8.65~173 μg/ml,平均回收率>99.27%,日内和日间RSD<5%。 结论 本法快速、简便、准确,适合于临床血药浓度监测。
Abstract:
Objective To establish a determination method for valprate in serum. Methods Serum sample was acidified by sulfuric acid and extracted with ethyl ether, cyclohexanecarboxylic acid was selected as an internal standard, α-bromoacetophenone as derivative reagent. Determination was performed with HPLC with methanol:water (70:30) as the mobile phase.The nalytical column was Eclipse Plus C18 (150 mm×4.6 mm, 5 μm), detected at 248 nm, the flow rate was 1.0 ml/min. Results The linear rang of valproate was 8.65~173 μg/ml. The mean relative recovery was bigger than 99.27%. Both the relative standard deviation (RSD) of inter-day and intra-day was less than 5%. Conclusions The method is rapid, accurate, sensitive and suitable for clinical therapeutic drug monitoring.
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