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四逆汤治疗甲状腺功能减退症的血清代谢组学研究

贾雨婷,  靳雨晨,  谭光国

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文章导航 > 药学实践与服务  > 2016 >  34(3): 237-240,248
贾雨婷, 靳雨晨, 谭光国. 四逆汤治疗甲状腺功能减退症的血清代谢组学研究[J]. 药学实践与服务, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
引用本文: 贾雨婷, 靳雨晨, 谭光国. 四逆汤治疗甲状腺功能减退症的血清代谢组学研究[J]. 药学实践与服务, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
shu
JIA Yuting, JIN Yuchen, TAN Guangguo. Study on Sini decoction in treatment of hypothyroidism by metabonomic method[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
Citation: JIA Yuting, JIN Yuchen, TAN Guangguo. Study on Sini decoction in treatment of hypothyroidism by metabonomic method[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
shu

四逆汤治疗甲状腺功能减退症的血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2016.03.011
  • 1.

    第四军医大学学员一旅, 陕西 西安 710032

  • 2.

    第四军医大学药学系药物分析学教研室, 陕西 西安 710032

基金项目: 国家自然科学基金项目(81402888);全军医学科技青年培育项目(13QNT197);陕西自然科学基金项目(2015JM8458)

Study on Sini decoction in treatment of hypothyroidism by metabonomic method

  • 1.

    Fourth Military Medical University, First Cadets Brigade, Xi'an 710032, China

  • 2.

    Fourth Military Medical University, Department of Pharmaceutical Analysis, School of Pharmacy, Xi'an 710032, China

  • 摘要: 目的 利用血清代谢组学鉴定甲状腺功能减退症(甲减)相关的代谢变化,评价四逆汤治疗甲减的作用。 方法 将24只大鼠分为假手术组、模型组和四逆汤治疗组,采用甲状腺切除诱导甲减模型,给予4周相应的治疗后,取大鼠血清进行超高效液相色谱-四极杆-飞行时间质谱仪(UPLC-Q-TOFMS)分析,结合模式识别分析各组间代谢物差异,评价四逆汤的治疗作用。 结果 血清代谢组学分析鉴定了9种甲减相关的生物标志物,涉及三羧酸循环、苯丙氨酸代谢、鞘脂代谢和磷脂代谢,同时数据显示四逆汤对甲减有改善治疗作用。 结论 四逆汤能够通过调节失衡的三羧酸循环、苯丙氨酸代谢和磷脂代谢而发挥治疗甲减的作用。
    Abstract: Objective To evaluate therapeutic effects of Sini decoction (SND) on hypothyroidism based on serum metabonomics method. Methods Twenty-four rats were randomly divided into three groups including sham group, surgery-induced hypothyroid model group and SND-treated group. The SND-treated group was given SND for 4 weeks. After 4 weeks, the serum samples of each rat were collected and analyzed by UHPLC-Q-TOFMS, pattern recognition analysis of metabolomics differences among the groups and therapeutic effects of SND were evaluated. Results Total of 9 metabolites were identified through serum metabonomics analysis. It was showed that there was a possible mechanism that hypothyroidism was mainly involved in citrate cycle, phenylalanine metabolism, sphingolipid metabolism and phospholipid metabolism and the damage of hypothyroidism was reversed by SND. Conclusion SND administration could provide satisfactory effects on hypothyroidism through partially regulating the perturbed citrate cycle, phenylalanine metabolism and phospholipid metabolism.
  • [1] Hulbert AJ. Thyroid hormones and their effects:a new perspective[J]. Biol Rev Camb Philos Soc, 2000,75(4):519-631.
    [2] Flynn RW, Bonellie SR, Jung RT, et al. Serum thyroid-stimulating hormone concentration and morbidity from cardiovascular disease and fractures in patients on long-term thyroxine therapy[J]. J Clin Endocrinol Metab, 2010,95(1):186-193.
    [3] La Vignera S, Vicari E, Tumino S, et al. L-thyroxin treatment and post-menopausal osteoporosis:relevance of the risk profile present in clinical history[J]. Minerva Ginecol, 2008,60(6):475-484.
    [4] 蔡定芳, 陈晓红, 刘彦芳, 等. 探讨阴中求阳与阳中求阴对谷氨酸单钠大鼠下丘脑-垂体-肾上腺-胸腺轴的影响[J]. 中国中西医结合杂志,1999,19(7):415-417.
    [5] Tan G, Zhu Z, Jing J, et al. Characterization of constituents in Sini decoction and rat plasma by high-performance liquid chromatography with diode array detection coupled to time-of-flight mass spectrometry[J]. Biomed Chromatogr, 2011, 25(8):913-924.
    [6] Guo L, Tan G, Liu P, et al. Three plasma metabolite signatures for diagnosing high altitude pulmonary edema[J]. Sci Rep, 2015, 5:15126.
    [7] Wang M, Lamers RJ, Korthout HA, et al. Metabolomics in the context of systems biology:bridging traditional Chinese medicine and molecular pharmacology[J]. Phytother Res, 2005, 19(3):173-182.
    [8] Wu S, Tan G, Dong X, et al. Metabolic profiling provides a system understanding of hypothyroidism in rats and its application[J]. PLoS One, 2013, 8(2):e55599.
    [9] 国家药典委员会. 中华人民共和国药典2015年版一部[S].北京:中国医药科技出版社,2015:789.
    [10] Alzoubi KH, Gerges NZ, Aleisa AM, et al. Levothyroxin restores hypothyroidism-induced impairment of hippocampus-dependent learning and memory:Behavioral, electrophysiological, and molecular studies[J]. Hippocampus, 2009, 19(1):66-78.
    [11] Tan G, Lou Z, Liao W, et al. Hydrophilic interaction and reversed-phase ultraperformance liquid chromatography TOF-MS for serum metabonomic analysis of myocardial infarction in rats and its applications[J]. Mol Biosyst, 2012, 8(2):548-556.
    [12] Briere JJ, Favier J, Gimenez-Roqueplo AP, et al. Tricarboxylic acid cycle dysfunction as a cause of human diseases and tumor formation[J]. Am J Physiol Cell Physiol, 2006, 291(6):C1114-C1120.
    [13] Sjoberg S, Eriksson M, Nordin C. L-thyroxine treatment and neurotransmitter levels in the cerebrospinal fluid of hypothyroid patients:a pilot study[J]. Eur J Endocrinol, 1998, 139(5):493-497.
    [14] Babenko NA. Long- and short-term effects of thyroxine on sphingolipid metabolism in rat liver[J]. Med Sci Monit, 2005,11(5):Br131-138.
    [15] Xu Y. Sphingosylphosphorylcholine and lysophosphatidylcholine:G protein-coupled receptors and receptor-mediated signal transduction[J]. Biochim Biophys Acta, 2002,1582(1-3):81-88.
    [16] Wang L, Radu CG, Yang LV, et al. Lysophosphatidylcholine-induced surface redistribution regulates signaling of the murine G protein-coupled receptor G2A[J]. Mol Biol Cell, 2005, 16(5):2234-2247.
    [17] Zelinskaia NB. The significance of studying phospholipids in the diagnosis of hypothyroidism[J]. Vrach Delo, 1989, (5):79-81.
    [18] Raffelt K, Moka D, Sullentrop F, et al. Systemic alterations in phospholipid concentrations of blood plasma in patients with thyroid carcinoma:an in-vitro (31)P high-resolution NMR study[J]. NMR Biomed, 2000,13(1):8-13.
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  • 收稿日期:  2016-03-14
  • 修回日期:  2016-04-22

四逆汤治疗甲状腺功能减退症的血清代谢组学研究

doi: 10.3969/j.issn.1006-0111.2016.03.011
  • 1. 第四军医大学学员一旅, 陕西 西安 710032
  • 2. 第四军医大学药学系药物分析学教研室, 陕西 西安 710032
    • 基金项目:  国家自然科学基金项目(81402888);全军医学科技青年培育项目(13QNT197);陕西自然科学基金项目(2015JM8458)
  • 收稿日期: 2016-03-14
  • 修回日期: 2016-04-22

摘要: 目的 利用血清代谢组学鉴定甲状腺功能减退症(甲减)相关的代谢变化,评价四逆汤治疗甲减的作用。 方法 将24只大鼠分为假手术组、模型组和四逆汤治疗组,采用甲状腺切除诱导甲减模型,给予4周相应的治疗后,取大鼠血清进行超高效液相色谱-四极杆-飞行时间质谱仪(UPLC-Q-TOFMS)分析,结合模式识别分析各组间代谢物差异,评价四逆汤的治疗作用。 结果 血清代谢组学分析鉴定了9种甲减相关的生物标志物,涉及三羧酸循环、苯丙氨酸代谢、鞘脂代谢和磷脂代谢,同时数据显示四逆汤对甲减有改善治疗作用。 结论 四逆汤能够通过调节失衡的三羧酸循环、苯丙氨酸代谢和磷脂代谢而发挥治疗甲减的作用。

English Abstract

贾雨婷, 靳雨晨, 谭光国. 四逆汤治疗甲状腺功能减退症的血清代谢组学研究[J]. 药学实践与服务, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
引用本文: 贾雨婷, 靳雨晨, 谭光国. 四逆汤治疗甲状腺功能减退症的血清代谢组学研究[J]. 药学实践与服务, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
shu
JIA Yuting, JIN Yuchen, TAN Guangguo. Study on Sini decoction in treatment of hypothyroidism by metabonomic method[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
Citation: JIA Yuting, JIN Yuchen, TAN Guangguo. Study on Sini decoction in treatment of hypothyroidism by metabonomic method[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 237-240,248. doi: 10.3969/j.issn.1006-0111.2016.03.011
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