灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用

许燕; 胡明慧; 高蓓蓉; 周选围

doi:10.3969/j.issn.1006-0111.2016.03.008

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用

许燕, 胡明慧, 高蓓蓉, 周选围

文章导航 > 药学实践与服务 > 2016 > 34(3): 223-226,277

许燕, 胡明慧, 高蓓蓉, 周选围. 灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用[J]. 药学实践与服务, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008

引用本文:

XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008

Citation:

XU Yan, HU Minghui, GAO Beirong, ZHOU Xuanwei. Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro[J]. Journal of Pharmaceutical Practice and Service, 2016, 34(3): 223-226,277. doi: 10.3969/j.issn.1006-0111.2016.03.008

灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用

doi: 10.3969/j.issn.1006-0111.2016.03.008

1.
阿斯利康中国创新中心, 上海 201203;复旦大学生命科学学院, 上海 200438
2.
阿斯利康中国创新中心, 上海 201203
3.
上海交通大学农业与生物学院, 上海 200240

基金项目: 国家自然科学基金面上项目(30771500)

Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

1.
AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China;School of Life Sciences, Fudan University, Shanghai 200438, China
2.
AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China
3.
School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China

摘要: 目的通过体外试验测定了3种灵芝免疫调节蛋白(FIP-glu、FIP-gat和FIP-SN15)对26株人胃癌细胞抑制增殖的影响。方法采用MTS比色法检测了3种灵芝免疫调节蛋白对26株胃癌细胞的抑制作用,计算出每种灵芝免疫调节蛋白对不同胃癌细胞50%生长抑制所需的药物浓度值(GI₅₀)。结果灵芝免疫调节蛋白加入72 h后,发现3种真菌免疫调节蛋白分别对7株胃癌细胞株NUGC-3、GTL-16、HGC-27、IM95m、SNU-638、SNU-216和SNU-5表现出较强的抑制作用,其GI₅₀<50 μg/ml。只对2株胃癌细胞NUGC-4和OCUM-1没有抑制作用。结论灵芝免疫调节蛋白在体外对大多数人胃癌细胞株有较强的抑制作用,3种灵芝免疫调节蛋白的作用趋势相同,有进一步开发成抗胃癌药物的潜力。
- 灵芝 /
- 真菌免疫调节蛋白 /
- 胃癌细胞 /
- 细胞增殖 /
- 细胞毒性 /
- MTS比色法
Abstract: Objective To study the cell proliferative effects of fungal immunomodulatory proteins from Ganoderma spp. on 26 gastric cancer cell lines in vitro. Method 26 human gastric cancer cell lines were treated with FIPs by MTS assay. The average optical density(OD) in 490 nm and inhibition rate(GI₅₀)was counted by Universal Microplate Spectrophotometer. Results Three FIPs showed similar profiling in 26 human gastric cancer cell lines after 72 h treatment in cell proliferation assay, which except for NUGC-4 and OCUM-1 did not showed obvious anti-proliferative effect, the other 24 human gastric cell lines showed some anti-proliferative effects, especially for 7 cell lines(NUGC-3, GTL-16, HGC-27, IM95m, SNU-638, SNU-216 and SNU-5) showing strong potency, with their GI₅₀ less than 50 μg/ml. Conclusion FIPs showed strong anti-proliferative effects in some human gastric cancer cell lines in vitro, which had potential to be further developed as anti-gastric cancer drugs.
- Ganoderma spp. /
- fungal immunomodulatory proteins(FIPs) /
- gastric cancer cell lines /
- cell proliferation /
- cytotoxicity /
- MTS colorimetric assay

[1]	苏恺琪, 王雪飞, 周选围. 黑灵芝免疫调节蛋白基因的克隆和生物信息学分析[J]. 上海交通大学学报(农业科学版), 2012, 30(1):65-71.
[2]	Li F, Wen H, Zhang Y, et al. Purification and characterization of a novel immunomodulatory protein from the medicinal mushroom Trametes versicolor [J]. Sci China Life Sci, 2011, 54(4):379-385.
[3]	Wang XF, Li QZ, Bao TW, et al. In vitro rapid evolution of fungal immunomodulatory proteins by DNA family shuffling[J]. Appl Microbiol Biotechnol, 2013, 97(6):2455-2465.
[4]	Li QZ, Huang L, Wang XF, et al. Fungal immunomodulatory protein from Flammulina velutipes induces cytokine gene expression in mouse spleen cells[J]. Curr Top Nutraceut R, 2011, 9(3):111-118.
[5]	Li QZ, Wang XF, Zhou XW. Recent status and prospects of the fungal immunomodulatory protein family[J]. Crit Rev Biotechnol, 2011, 31(4):365-375.
[6]	王晓林, 梁重阳, 李泓睿, 等. 灵芝免疫调节蛋白(rLZ-8)诱导K562细胞发生细胞核介导的细胞凋亡的研究[J]. 中国免疫学杂志, 2010, 26(7):616-618.
[7]	Liao CH, Hsiao YM, Lin CH, et al. Induction of premature senescence in human lung cancer by fungal immunomodulatory protein from Ganoderma tsugae[J]. Food Chem Toxicol, 2008, 46(5):1851-1859.
[8]	Cong WR, Xu H, Liu Y, et al. Production and functional characterization of a novel fungal immunomodulatory protein FIP-SN shuffled from two genes of Ganoderma species[J]. App Microbiol Biotechnol, 2014, 98(13):5967-5975.
[9]	刘建文.抗肿瘤药理实验方法[M].北京:化学工业出版社,2010:29.
[10]	司徒镇强, 吴军正. 细胞培养[M]. 北京:世界图书出版社西安公司, 1996:135.
[11]	Tanaka M, Obata T, Sasaki T.Evaluation of antitumour effects of docetaxel (Taxotere) on human gastric cancers in vitro and in vivo [J]. Eur J Cancer, 1996, 32A (2):226-230.
[12]	Wang XF, Su KQ, Bao TW, et al. Immunomodulation effects of fungal proteins[J]. Curr Top Nutraceut R, 2012, 10(1):1-11.
[13]	Kino K, Yamashita A, Yamaoka K, et al. Isolation and characterization of a new immunomedulatory protein, ling zhi-8(LZ-8), from Ganoderma lucidum [J]. J Biol Chem, 1989, 264:472-478.
[14]	Lin WH, Huang CH, Hsu CI, et al. Dimerization of the N-terminal amphipathic alpha-helix domain of the fungal immunomodulatory protein from Ganoderma tsugae (Fip-gts) defined by a yeast two-hybrid system and site-directed mutagenesis[J]. J Biol Chem, 1997, 272(32):20044-20048.
[15]	Ishiyama M, Tominaga H, Shiga M, et al. Novel cell proliferation and cytotoxicity assays using a tetrazolium salt that produces a comparison of multiple cytotoxicity assays 61 water-soluble formazan dye[J]. In Vitro Toxicol, 1995, 8(2):187-190.

[1]	石晓萍, 吕迁洲, 李晓宇, 许青. 泊沙康唑对比伏立康唑经验治疗或诊断驱动治疗免疫功能低下患者侵袭性霉菌病的成本-效果分析 . 药学实践与服务, 2024, 42(): 1-8. doi: 10.12206/j.issn.2097-2024.202401050
[2]	唐淑慧, 凤美娟, 薛智霞, 鲁桂华. 帕博利珠单抗治疗所致免疫相关不良反应与中医体质的相关性研究 . 药学实践与服务, 2024, 42(5): 217-222. doi: 10.12206/j.issn.2097-2024.202311029
[3]	顾佳钰, 胡馨儿, 王晓飞, 张颖, 张海, 曹岩. 侧流免疫层析定量检测方法的研究进展 . 药学实践与服务, 2024, 42(7): 273-277, 284. doi: 10.12206/j.issn.2097-2024.202307037
[4]	马兹芬, 许维恒, 金煜翔, 薛磊. 食管癌的靶向治疗与免疫治疗研究进展 . 药学实践与服务, 2024, 42(6): 231-237. doi: 10.12206/j.issn.2097-2024.202306008
[5]	刘汝雄, 杨万镇, 涂杰, 盛春泉. 铁死亡调控蛋白GPX4的小分子抑制剂研究进展 . 药学实践与服务, 2024, 42(9): 375-378. doi: 10.12206/j.issn.2097-2024.202312075
[6]	刘丽艳, 余小翠, 孙传铎. 纳武利尤单抗治疗非小细胞肺癌有效性及安全性的Meta分析 . 药学实践与服务, 2024, 42(10): 451-456. doi: 10.12206/j.issn.2097-2024.202310044
[7]	迟文雅, 袁艳, 李伟林, 吴茼妤, 俞媛. 负载骨髓间充质干细胞/白藜芦醇脂质体的水凝胶支架用于创伤性脑损伤治疗 . 药学实践与服务, 2024, 42(): 1-8. doi: 10.12206/j.issn.2097-2024.202406034
[8]	宋雨桐, 夏德润, 顾珩, 唐少文, 易洪刚, 沃红梅. 帕博利珠单抗与铂类化疗方案在晚期非小细胞肺癌一线治疗中的药物经济学评价 . 药学实践与服务, 2024, 42(8): 334-340. doi: 10.12206/j.issn.2097-2024.202303023
[9]	修建平, 杨朝爱, 刘禧澳, 潘乾禹, 韦广旭, 王卫星. 全反式维甲酸对肝星状细胞活化及氧化应激的作用和机制探索 . 药学实践与服务, 2024, 42(7): 291-296. doi: 10.12206/j.issn.2097-2024.202312054
[10]	姜涛, 徐卫凡, 蒋益萍, 夏天爽, 辛海量. 巴戟天丸组方对Aβ损伤成骨细胞的作用及基于网络药理学的机制研究 . 药学实践与服务, 2024, 42(7): 285-290, 296. doi: 10.12206/j.issn.2097-2024.202305011
[11]	杨媛媛, 安晓强, 许佳捷, 江键, 梁媛媛. 正极性驻极体联合5-氟尿嘧啶对瘢痕成纤维细胞生长抑制的协同作用 . 药学实践与服务, 2024, 42(6): 244-247. doi: 10.12206/j.issn.2097-2024.202310027
[12]	冯志惠, 邓仪卿, 叶冰, 安培, 张宏, 张海军. 雀梅藤石油醚提取物诱导三阴性乳腺癌细胞凋亡的实验研究 . 药学实践与服务, 2024, 42(6): 253-259. doi: 10.12206/j.issn.2097-2024.202311055

点击查看大图

计量

文章访问数: 3401
HTML全文浏览量: 372
PDF下载量: 7
被引次数: 0

灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用

doi: 10.3969/j.issn.1006-0111.2016.03.008

1. 阿斯利康中国创新中心, 上海 201203;复旦大学生命科学学院, 上海 200438

2. 阿斯利康中国创新中心, 上海 201203

3. 上海交通大学农业与生物学院, 上海 200240

基金项目: 国家自然科学基金面上项目(30771500)

收稿日期: 2015-06-26
修回日期: 2015-10-21

关键词:

灵芝 /

摘要: 目的通过体外试验测定了3种灵芝免疫调节蛋白(FIP-glu、FIP-gat和FIP-SN15)对26株人胃癌细胞抑制增殖的影响。方法采用MTS比色法检测了3种灵芝免疫调节蛋白对26株胃癌细胞的抑制作用,计算出每种灵芝免疫调节蛋白对不同胃癌细胞50%生长抑制所需的药物浓度值(GI₅₀)。结果灵芝免疫调节蛋白加入72 h后,发现3种真菌免疫调节蛋白分别对7株胃癌细胞株NUGC-3、GTL-16、HGC-27、IM95m、SNU-638、SNU-216和SNU-5表现出较强的抑制作用,其GI₅₀<50 μg/ml。只对2株胃癌细胞NUGC-4和OCUM-1没有抑制作用。结论灵芝免疫调节蛋白在体外对大多数人胃癌细胞株有较强的抑制作用,3种灵芝免疫调节蛋白的作用趋势相同,有进一步开发成抗胃癌药物的潜力。

English Abstract

Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

1. AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China;School of Life Sciences, Fudan University, Shanghai 200438, China

2. AstraZeneca, Asia & Emerging Markets iMed, AstraZeneca Global R&D, Shanghai 201203, China

3. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China

Received Date: 2015-06-26
Rev Recd Date: 2015-10-21

Keywords:

Ganoderma spp. /
fungal immunomodulatory proteins(FIPs) /
gastric cancer cell lines /
cell proliferation /
cytotoxicity /
MTS colorimetric assay

Abstract: Objective To study the cell proliferative effects of fungal immunomodulatory proteins from Ganoderma spp. on 26 gastric cancer cell lines in vitro. Method 26 human gastric cancer cell lines were treated with FIPs by MTS assay. The average optical density(OD) in 490 nm and inhibition rate(GI₅₀)was counted by Universal Microplate Spectrophotometer. Results Three FIPs showed similar profiling in 26 human gastric cancer cell lines after 72 h treatment in cell proliferation assay, which except for NUGC-4 and OCUM-1 did not showed obvious anti-proliferative effect, the other 24 human gastric cell lines showed some anti-proliferative effects, especially for 7 cell lines(NUGC-3, GTL-16, HGC-27, IM95m, SNU-638, SNU-216 and SNU-5) showing strong potency, with their GI₅₀ less than 50 μg/ml. Conclusion FIPs showed strong anti-proliferative effects in some human gastric cancer cell lines in vitro, which had potential to be further developed as anti-gastric cancer drugs.

引用本文:

Citation:

全文HTML

参考文献 (15)

下载: 全尺寸图片幻灯片

返回文章

用微信扫码二维码

分享至好友和朋友圈

主管、主办: 中国人民解放军海军军医大学地址: 上海市杨浦区国和路325号邮政编码: 200433

电话: （021）81871324,81871397 E-mail: [email protected]

网站备案号沪ICP备05003363号-1

本系统由北京仁和汇智信息技术有限公司开发

留言板

灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用

doi: 10.3969/j.issn.1006-0111.2016.03.008

Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

计量

出版历程

灵芝真菌免疫调节蛋白在体外对不同胃癌细胞的抑制作用

doi: 10.3969/j.issn.1006-0111.2016.03.008

1. 阿斯利康中国创新中心, 上海 201203;复旦大学生命科学学院, 上海 200438 2. 阿斯利康中国创新中心, 上海 201203 3. 上海交通大学农业与生物学院, 上海 200240

English Abstract

Inhibition effects of fungal immunomodulatory proteins from Ganoderma spp. on different gastric cancer cell lines in vitro

全文HTML

目录

1. 阿斯利康中国创新中心, 上海 201203;复旦大学生命科学学院, 上海 200438

2. 阿斯利康中国创新中心, 上海 201203

3. 上海交通大学农业与生物学院, 上海 200240