The chemical constituents and antioxidant activity of Ixeris sonchifolia
-
摘要: 目的 对菊科(Compositae)苦荬菜属 (Ixeris)植物抱茎苦荬菜正丁醇萃取层中的化学成分进行分离与鉴定。 方法 采用反复硅胶柱色谱﹑重结晶、Sephadex LH-20柱色谱﹑开放ODS柱色谱、HPLC等方法进行分离纯化以获得单体化合物。通过理化性质及波谱数据鉴定这些单体化合物的化学结构,利用1,1-二苯基-2-三硝基苯肼(DPPH)和2,2-联氮基-双-(3-乙基苯并噻唑啉-6-磺酸)二氨盐(ABTS)法评价这些化合物清除自由基的活性。 结果 从抱茎苦荬菜正丁醇萃取层中分离得到5个化合物,分别鉴定为(+)-松脂素-4-O-β-D-葡萄糖苷( 1 )、glochidioboside ( 2 )、3,4,5-trimethoxyphenyl-1-O-β-D-glucopy-ranoside( 3 )、芒柄花苷( 4 )、大豆素( 5 )、木犀草素( 6 )、芹菜素( 7 )。利用DPPH和ABTS法测试了这7个化合物清除自由基的活性。 结论 化合物 1 ~ 5 为首次从苦荬菜属植物中分离得到,化合物 1 、 2 、 4 ~ 7 显示了较强的自由基清除活性。Abstract: Objective To study the chemical constituents of Ixeris sonchifolia. Methods Monomeric compounds were isolated by chromatography on silica gel column, Sephadex LH-20, ODS chromatography column and HPLC.Thestructure of the compound wasconfirmed on the basis of physio-chemical constants and spectroscopic analysis. 2,2-diphenyl-1-pikrylhydrazyl (DPPH) and 3-ethylbenzthiazoline-6-sulfonic acid(ABTS)assay were usedto evaluate the antioxidant activities of isolated compounds. Results Five compounds were isolated from 70% ethanol-water extraction and their structures were identified as (+)-pinoresinol-4-O-β-D-glucopyranoside( 1 ), glochidioboside( 2 ), 3,4,5-trimethoxyphenyl-1-O-β-D-glucopy-ranoside( 3 ), ononin( 4 ),daidzein( 5 ), luteolin ( 6 ), and apigenin ( 7 ). Meanwhile, antioxidant activities of all the isolates were evaluated by DPPHand ABTS. Conclusion Compounds 1-5 were isolated from this genus for the first time. The active results showed that 1, 2, 4-7 exhibited potent antioxidant activity.
-
[1] 江苏新医学院. 中药大辞典[M]. 上海: 上海人民出版社, 1977: 1300. [2] 封锡志.抱茎苦荬菜的化学成分和生物活性的研究[D]. 沈阳:沈阳药科大学,2001: 2-29. [3] 侯 晶, 艾瑞婷, 周凌艳, 等. 苦碟子化学成分的分离与鉴定[J]. 沈阳药科大学学报, 2011, 28(11): 879-881. [4] Rahman MMA,Dewick PM, Jackson DE,et al. Lignans of Forsythia intermedia[J]. Phytochemistry,1990, 29:1971-1980. [5] Yoshio T, Chieko M, Toshiya M,et al. Glochidioboside, a glucoside of (7S,8R)-dihydro-dehydrodiconiferyl alcohol from leaves of Glochidion obovatum[J]. Phytochemistry, 1998, 49(7):2137-2139. [6] Wang RR, Gu Q, Wang YH, et al.Anti-HIV-1 activities of compounds isolated from the medicinal plant Rhus chinensis[J].J Ethnopharmacol,2008, 117(2):249-256. [7] Meera B, Kalidhar SB. Phytochemical evaluation of Pongamia pinnata L. seeds oil[J]. Indian J Pharm, 2004, 66(4): 447-448. [8] Yadav PP, Ahmad G, MauryaR. Furanoflavonoids from Pongamia pinnata fruits[J]. Phytochemistry, 2004, 65(4):439-443. [9] Deng J, Cheng W, Yang G. A novel antioxidant activity index (AAU) for natural products using the DPPH assay[J]. Food Chem, 2011, 125: 1430-1435. [10] Sharma OP, Bhat TK. DPPH antioxidant assay revisited[J]. Food Chem,2009, 113: 1202-1205. [11] Wu SB, Dastmalchi K, Long C, et al. Metabolite profiling of jaboticaba (Myrciaria cauliflora) and other dark-colored fruit juices[J]. J Agric Food Chem, 2012, 60, 7513-7525. -
点击查看大图
计量
- 文章访问数: 3720
- HTML全文浏览量: 342
- PDF下载量: 125
- 被引次数: 0
下载:
