芎归汤主要化学成分的HPLC-TOF-MS分析

吕亚男; 谭光国

doi:10.3969/j.issn.1006-0111.2015.01.012

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

x 关闭永久关闭

应中央军委要求，2022年9月起，《药学实践杂志》将更名为《药学实践与服务》，双月刊，正文96页；2023年1月起，拟出版月刊，正文64页，数据库收录情况与原《药学实践杂志》相同。欢迎作者踊跃投稿！

芎归汤主要化学成分的HPLC-TOF-MS分析

吕亚男, 谭光国

文章导航 > 药学实践与服务 > 2015 > 33(1): 49-52,71

吕亚男, 谭光国. 芎归汤主要化学成分的HPLC-TOF-MS分析[J]. 药学实践与服务, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012

引用本文:

吕亚男, 谭光国. 芎归汤主要化学成分的HPLC-TOF-MS分析[J]. 药学实践与服务, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012

LÜ Yanan, TAN Guangguo. Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012

Citation:

LÜ Yanan, TAN Guangguo. Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS[J]. Journal of Pharmaceutical Practice and Service, 2015, 33(1): 49-52,71. doi: 10.3969/j.issn.1006-0111.2015.01.012

芎归汤主要化学成分的HPLC-TOF-MS分析

doi: 10.3969/j.issn.1006-0111.2015.01.012

解放军22医院, 青海格尔木 816000

基金项目: 全军医学科技青年培育项目(13QNT197)

Analysis on chemical constituents of Xionggui Decotion by HPLC-TOF-MS

No.22 Hospital of PLA, Golmud 816000, China

摘要: 目的通过高效液相色谱-飞行时间质谱(HPLC-TOF-MS)联用技术,对中药复方芎归汤中的主要化学成分进行有效鉴定。方法色谱分离采用资生堂 CAPCELL PAK C₁₈反相柱(100 mm×3.0 mm,3 μm),流动相组成分别为0.1%甲酸水溶液和乙腈,梯度洗脱,流速为0.4 ml/min;质谱定性采用飞行时间质谱,正离子模式扫描。结果在优化的液质联用条件下,通过飞行时间质谱鉴定了芎归汤中47种主要成分,并对其药材来源进行了归属。结论通过HPLC-TOF-MS联用技术,为鉴定芎归汤中的化学成分建立起了一种快速、高效的分析方法。
- 芎归汤 /
- 高效液相色谱-飞行时间质谱 /
- 化学成分
Abstract: Objective To analyze chemical constituents of Xionggui Decotion by rapid-resolution (high performance) liquid chromatography-time of flight mass spectrometry (HPLC-TOF-MS). Methods A Shelloseido column(100 mm×3.0 mm,3 μm)was used to separate.The mobile phase consisted of water containing 0.1% methane acid and acetonitrile was used as gradient elute.The flow rate was 0.4 ml/min.TOF-MS was applied for qualitative analysis under positive ion mode. Results Under LC/MS condition,47 major constituents in Xionggui Decotion were identified by time of flight mass spectrometry and structure-relevant fragment ions. Conclusion A simple and reliable method using HPLC-TOF-MS was established to identify the chemical constituents of Xionggui Decotion.
- Xionggui Decotion /
- HPLC-TOF-MS /
- chemical constituents

[1]	陈立峰,徐琳本,王晓洪,等.加味芎归汤对大鼠局灶性脑缺血损伤的保护作用和血液流变学的影响[J].中国实验方剂学杂志,2010,16(11):157-164.
[2]	王志琪,王晓洪,陈立峰.加味芎归汤对大鼠全脑缺血再灌注损伤的保护作用[J].中国实验方剂学杂志,2011,17(15):206-210.
[3]	Zhan ZJ,Fan CQ,Ding J,et al.Novel diterpenoids with potent inhibitory activity against endothelium cell HMEC and cytotoxic activities from a well-known TCM plant Daphne genkwa[J].Bioorg Med Chem,2005,13(31):645-655.
[4]	Zhu Z,Zhang H,Zhao L,et al.Rapid separation and identification of phenolic and diterpenoid constituents from Radix Salvia miltiorrhizae by high-performance liquid chromatography diode-array detection,electrospray ionization time-of-flight mass spectrometry and electrospray ionization quadrupole ion trap mass spectrometry[J].Rapid Commun Mass Spectr,2007,21(12):1855-1865.
[5]	Zhao L,Lou ZY,Zhu ZY,et al.Characterization of constituents in Stellera chamaejasme L.by rapid-resolution liquid chromatography-diode array detection and electrospray ionization time-of-flight mass spectrometry[J].Biomed Chromatogr,2008,22(11):64-72.
[6]	Li SL,Chan SK,Lin G,et al.Simultaneous analysis of seventeen chemical ingredients of Ligusticum chuanxiong by on-line high performance liquid chromatography-diode array detector-mass spectrometry[J].Plant Med,2003,69(5):445-451.
[7]	Liang MJ,He LC,Yang GD.Screening,analysis and in vitro vasodilatation of effective components from Ligusticum chuanxiong[J].Life Sci,2005,78(2):128-133.
[8]	Peng Z,Bi ZM,Li P,et al.LC-DAD-MS determination of the major constituents in Radix Angelicae sinensis[J].Chromatographia, 2008,67:973-978.
[9]	Yi L,Liang Y,Wu H,et al.The analysis of Radix Angelicae sinensis(Danggui)[J].J Chromatogr A,2009,1216(11):1991-2001.
[10]	Lin LZ,He XG,Lian LZ,et al.Liquid chromatographic-electrospray mass spectrometric study of the phthalides of Angelica sinensis and chemical changes of Z-ligustilide[J].J Chromatogr A,1998,810:71-79.
[11]	Lu GH,Kelvin C,Liang YZ,et al.Development of high-performance liquid chromatographic fingerprints for distinguishing Chinese Angelica from related umbelliferae herbs[J].J Chromatogr A,2005,1073(1-2):383-392.
[12]	Li SL,Lin G,Chung HS,et al.Study on fingerprint of Rhizome chuanxiong by HPLC-DAD-MS[J].Acta Pharm Sin,2004,39(8):621-626.
[13]	Lao SC,Li SP,Kelvin K,et al.Identification and quantification of 13 components in Angelica sinensis(Danggui)by gas chromatography mass spectrometry coupled with pressurized liquid extraction[J].Anal Chim Acta,2004,526:131-137.

[1]	夏哲炜, 曾垣烨, 朱海菲, 李育, 陈啸飞. 核磁共振磷谱法测定磷酸氢钙咀嚼片中药物含量 . 药学实践与服务, 2024, 42(9): 399-401, 406. doi: 10.12206/j.issn.2097-2024.202404063
[2]	毛智毅, 王筱燕, 陈晓颖, 汤逸斐. 度拉糖肽联合二甲双胍对肥胖型2型糖尿病患者机体代谢、体脂成分及血清脂肪因子的影响 . 药学实践与服务, 2024, 42(7): 305-309. doi: 10.12206/j.issn.2097-2024.202305032
[3]	赖立勇, 夏天爽, 徐圣焱, 蒋益萍, 岳小强, 辛海量. 中药青蒿抗氧化活性的谱效关系研究 . 药学实践与服务, 2024, 42(5): 203-210, 216. doi: 10.12206/j.issn.2097-2024.202211012
[4]	陈静, 何瑞华, 翁月, 徐熠, 刘静, 黄瑾. 基于网络药理学和分子对接技术探究定清片活性成分治疗白血病的作用机制 . 药学实践与服务, 2024, 42(11): 479-486. doi: 10.12206/j.issn.2097-2024.202401073
[5]	舒飞, 孙蕊, 宋凯, 张元林, 闫家铭, 舒丽芯. 粉-液双室袋产品综合评估 . 药学实践与服务, 2024, 42(): 1-5. doi: 10.12206/j.issn.2097-2024.202312009
[6]	陈方剑, 骆锦前, 王志君, 胡叶帅, 孙煜昕, 宋洪杰. HPLC-MS/MS同时测定感冒安颗粒中5种黄酮成分的含量 . 药学实践与服务, 2024, 42(9): 402-406. doi: 10.12206/j.issn.2097-2024.202403030
[7]	李丹, 戴贤春, 王芳珍, 陈奕含, 杨萍, 刘继勇. HPLC-MS/MS测定当归六黄汤中4种不同成分的含量 . 药学实践与服务, 2024, 42(6): 248-252, 266. doi: 10.12206/j.issn.2097-2024.202305007
[8]	岳春华, 贲永光, 王海桥. 基于NLRP1炎症小体探讨百合知母汤抗抑郁的作用机制 . 药学实践与服务, 2024, 42(8): 325-333. doi: 10.12206/j.issn.2097-2024.202401033

点击查看大图

计量

文章访问数: 2991
HTML全文浏览量: 189
PDF下载量: 72
被引次数: 0

芎归汤主要化学成分的HPLC-TOF-MS分析

doi: 10.3969/j.issn.1006-0111.2015.01.012

解放军22医院, 青海格尔木 816000

基金项目: 全军医学科技青年培育项目(13QNT197)

收稿日期: 2013-08-09
修回日期: 2014-01-11

关键词:

芎归汤 /

高效液相色谱-飞行时间质谱 /

化学成分

摘要: 目的通过高效液相色谱-飞行时间质谱(HPLC-TOF-MS)联用技术,对中药复方芎归汤中的主要化学成分进行有效鉴定。方法色谱分离采用资生堂 CAPCELL PAK C₁₈反相柱(100 mm×3.0 mm,3 μm),流动相组成分别为0.1%甲酸水溶液和乙腈,梯度洗脱,流速为0.4 ml/min;质谱定性采用飞行时间质谱,正离子模式扫描。结果在优化的液质联用条件下,通过飞行时间质谱鉴定了芎归汤中47种主要成分,并对其药材来源进行了归属。结论通过HPLC-TOF-MS联用技术,为鉴定芎归汤中的化学成分建立起了一种快速、高效的分析方法。