Therapeutic effects of Thesium chinensis extracts on IgAN rats
-
摘要: 目的 考察百蕊草提取物对免疫球蛋白A肾病(IgAN)模型大鼠的治疗效果,以明确有效部位。 方法 采用水煎提取百蕊草,AB-8大孔树脂吸附,乙醇梯度洗脱制备各部位。给予大鼠口服牛血清蛋白(400 mg/kg),皮下注射蓖麻油、CCl4联合尾静脉注射脂多糖造模。从第8周起,对阳性组[雷公藤多苷(LGT),10 mg/kg]、水提取物组(TT,1.0 g/kg)、水溶性部位组(TTW,0.4 g/kg)、极性部位组(TT20,0.4 g/kg)和中等极性部位组大鼠(TT50,0.4 g/kg)每天灌胃给药,空白组和模型组给予生理盐水(n=8),连续5周。观察各组大鼠24 h尿量、24 h尿蛋白、尿沉渣红细胞计数,实验结束后进行血液生化指标检测、免疫荧光检测、肾组织的病理学检查。 结果 与空白组相比,模型组大鼠的24 h尿蛋白、尿沉渣中红细胞数量、血清中肌酐、尿素氮水平均显著升高。与模型组相比,TT组和TT50组的24 h尿蛋白(P<0.05)、尿沉渣中红细胞数量(P<0.01)、血清肌酐和尿素氮水平(P<0.05)降低。各给药组肾组织系膜区的免疫复合物沉积减少、肾小球增大、系膜基质增生、肾小管的损伤状况改善。 结论 百蕊草水提物及其中等极性部位对IgA肾病大鼠具有良好的治疗作用,其效果优于阳性对照雷公藤多苷。Abstract: Objective To evaluate the therapeutic effects of Thesium chinensis Turcz extracts on IgA nephropathy (IgAN) rats. Methods The hot water extracted solution of Thesium chinensis Turcz flow through the AB-8 macroreticular resin column repeatedly,then being eluted with different contents of ethanol to get four part of extract.The IgAN model of SD rats were established by oral intake of bovine serum albumin(BSA,400 mg/kg) together with injection of lipopolysaccharide(LPS) and CCl4.Beginning with 8th week,the positive group(LGT,10 mg/kg),water extract group(TT,1.0 g/kg),water soluble group(TTW,0.4 g/kg),high polarity group(TT20,0.4 g/kg) and medium polarity group(TT50,0.4 g/kg) rats were intragastric administrated drug daily,the normal group control and model control group rats were given with saline(n=8).During the next 5 weeks treatment process,the 24 h urine volume,24 h urine protein concentration,and quantity of erythrocyte in urine were observed.The levels of TP,ALP,ALT,AST,Scr and BUN in serum were determined.The expression of IgA deposition was measured by immunofluorescence staining,and the pathological changes of kidney tissue were assayed after the therapy process. Results After 5 weeks therapy,the 24 h urine protein concentration,the quantity of erythrocyte in urine,and the level of Scr and BUN of TT and TT50 groups were significantly lower than model control.The fluorescence intensities in the mesangial area were weaker than model control.Also,the degrees of renal capsule expansion and mesangial matrix proliferations,and the kidney tubules damage of TT and TT50 groups were weaker than those of model control. Conclusion The water extract and its medium polarity fraction of Thesium chinensis Turcz show good therapeutic effects on IgAN rats.
-
Key words:
- Thesium chinensis Turcz /
- IgAN /
- SD rats /
- TCD therapy
-
[1] Donadio JV,Grande JP.IgA nephropathy[J].N Engl J Med,2002,347(10):738-748. [2] 宣伟东,唐大海,卞 俊,等.百蕊草对阿霉素肾病大鼠治疗作用的实验研究[J].药学实践杂志,2012,30(6):443-446. [3] 汤 颖,娄探奇,成彩联,等.实验性IgA肾病模型的改进[J].中山大学学报(医学科学版),2006,27(2):184-187. [4] 彭胜男,曾慧红,傅爱香,等.大鼠IgA肾病模型肾脏变化分析[J].临床与实验病理学杂志,2012,28(5):482-485. [5] 张 君,姜 欣,王 莉,等.消斑愈肾颗粒对大鼠IgA肾病免疫调节的研究[J].中国实验方剂学杂志,2002,8(4):37-38. [6] 余英豪,郑志勇.IgA肾病分类的国际共识:2009牛津分类法介绍[J].临床与实验病理学杂志,2011,27(3):227-229. [7] Lee IIK,Kim KH,Choi SU,et al.Phytochemical constituents of Thesium chinense Turcz and their cytotoxic activities in vitro[J].Nat Prod Sci,2009,15(4):246-249. [8] 刘 洋,Parveen Z,邓玉林,等.百蕊草中黄酮类化合物的研究[J].中药材,2009,32(4):518-520. [9] Parveen Z,Deng Y,Saeed MK,et al.Antiinflammatory and analgesic activities of Thesium chinense Turcz extracts and its major flavonoids,kaempferol and kaempferol-3-O-glucoside[J].Yakugaru Zasshi,2007,127(8):1275-1279. [10] Wang H,Zhou CL,Lei H,et al.Kaempferol:a new immunosuppressant of calcineufin[J].IUBMB Life,2008,60(8):549-554. [11] Okamto I,Iwaki K,Koya-Miyata S,et al.The flavonoid kaempferol suppresses the graft-versus-host reaction by inhibiting type 1 cytokine production and CD8+ T cell engraftment[J].Clin Immunol,2002,103(2):132-144. [12] Parveen Z,Deng YL,Muhammad KS,et al.Optimizations of conditions for recovery of Astragalin from Thesium chinense Turcz[J].J Appl Sci,2006,6(13):2829-2832. -
点击查看大图
计量
- 文章访问数: 3203
- HTML全文浏览量: 245
- PDF下载量: 113
- 被引次数: 0
下载:
