抗肿瘤多肽蜂毒素的固相合成

李文娟; 李任武; 吴茂诚; 王傅喆; 秦丽萍; 李柏; 胡宏岗

doi:10.3969/j.issn.1006-0111.2014.01.013

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抗肿瘤多肽蜂毒素的固相合成

李文娟, 李任武, 吴茂诚, 王傅喆, 秦丽萍, 李柏, 胡宏岗

文章导航 > 药学实践与服务 > 2014 > 32(1): 51-52

李文娟, 李任武, 吴茂诚, 王傅喆, 秦丽萍, 李柏, 胡宏岗. 抗肿瘤多肽蜂毒素的固相合成[J]. 药学实践与服务, 2014, 32(1): 51-52. doi: 10.3969/j.issn.1006-0111.2014.01.013

引用本文:

LI Wenjuan, LI Renwu, WU Maocheng, WANG Fuzhe, QIN Liping, LI Bai, HU Honggang. Solid-phase synthesis of melittin of anti-tumor peptide[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 51-52. doi: 10.3969/j.issn.1006-0111.2014.01.013

Citation:

LI Wenjuan, LI Renwu, WU Maocheng, WANG Fuzhe, QIN Liping, LI Bai, HU Honggang. Solid-phase synthesis of melittin of anti-tumor peptide[J]. Journal of Pharmaceutical Practice and Service, 2014, 32(1): 51-52. doi: 10.3969/j.issn.1006-0111.2014.01.013

抗肿瘤多肽蜂毒素的固相合成

doi: 10.3969/j.issn.1006-0111.2014.01.013

1.
烟台大学药学院, 山东烟台 264005
2.
第二军医大学药学院有机化学教研室, 上海 200433
3.
第二军医大学长海医院中医系, 上海 200433

基金项目: 国家自然科学基金(20902109).

Solid-phase synthesis of melittin of anti-tumor peptide

1.
School of Pharmacy, Yantai University, Yantai 264005, China
2.
Department of Organic Chemistry, School of Pharmacy, Second Military Medical University, Second Military Medical University, Shanghai 200433, China
3.
Department of Traditional Chinese Medicine, Changhai Hospital, Second Military Medical University, Shanghai 200433, China

摘要: 目的合成具有抗肿瘤活性的二十六肽蜂毒素。方法采用Fmoc固相逐步化学合成,确立了以Wang树脂为固相载体,HOBt/DCC为缩合剂的合成工艺。结果通过本法能够顺利合成得到二十六肽蜂毒素,收率达32%。结论该合成方法具有可行性强、操作简便、总收率高等特点,适用于长链多肽的合成。
- 蜂毒素 /
- 固相合成 /
- 逐步缩合
Abstract: Objective To synthesize the antitumor activity of twenty-six peptide melittin. Methods The melittin was synthesized by stepwise solid-phase peptide synthesis (SPPS). During the synthesis of melittin, wang-resin was chosen as solid supporter and HOBt/DCC were used as the coupling reagent. Results The melittin was synthesized with yield of 32%. Conclusion The synthetic method was feasible, simple and with high yield for long chain polypeptide synthesis.
- melittin /
- solid-phase peptide synthesis /
- stepwise synthesis

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