摘要:
目的 探讨一种新型止血敷料—血余炭纳米纤维膜促进家兔创面愈合的作用。 方法 建立家兔背部双侧圆形创伤模型,每只兔子6个创面,将创面随机分为3组,分别给予医用纱布、血余炭纳米纤维膜和明胶海绵进行治疗,观察创面的愈合时间和愈合率,检测组织中羟脯氨酸含量的变化,并观察组织病理学变化。 结果 实验结果显示,血余炭纳米纤维膜具有较好的止血性能,显著缩短创面愈合时间,提高愈合率。血余炭纳米纤维膜组在给药后3、7、14 d的创面组织中羟脯氨酸含量均显著高于医用纱布对照组(P<0.01);在给药后7 d,血余炭纳米纤维膜组的创面组织中羟脯氨酸含量明显高于明胶海绵组(P<0.05)。组织学观察结果显示,给药后3、7 d,血余炭纳米纤维膜组的肉芽组织中成纤维细胞数量较医用纱布组和明胶海绵组均显著升高(P<0.05,P<0.01);血余炭纳米纤维膜组肉芽组织中毛细血管数量显著高于明胶海绵组和医用纱布组(P<0.05,P<0.01)。 结论 血余炭纳米纤维膜具有显著的促进皮肤创面愈合的作用。
Abstract:
Objective To study the effect of the new type of hemostat for nano-fibers tunic carbonized hair loaded drug (NTCH) on skin wound healing in rabbits. Methods Bilateral round wounds were made on the backs of rabbits.There were six wounds in each rabbit, treated with medical gauze, absorbable gelatin sponge and NCTH. The effect of NCTH on the skin wound was evaluated by the index of closure time and healing rate. The variation of histopathological characteristics and the content of hydroxyproline in the wound tissue were observed. Results Animal experimental results showed that the NCTH hemostasis performance was better, also significantly reduced closure time, and improve healing rate. The hydroxyproline content in the wound tissue in the NCTH group on days 3, 7, 14 post-treatment was higher than that in medical gauze group (P<0.01).7 days post-treatment, the hydroxyproline content in the wound tissue in the NCTH group was higher than that in gelatin sponge group (P<0.05).On histology observation, 3 days and 7 days post-treatment, the numbers of collagenoblast was contrasted, there was statistical difference among the NCTH group, gelatin sponge group and medical gauze group(P<0.05, P<0.01). 3 days and 7 days post-treatment, the numbers of newly-born blood capillary was contrasted, there was statistical significance difference among the NCTH group, gelatin sponge group and medical gauze group(P<0.05, P<0.01). Conclusion The NCTH had significantly acceleration effect on the skin wound healing.
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