摘要:
目的 建立同时测定利湿口服液(车前草、地肤子、金银花、陈皮、苦参、茯苓皮、麦饭石)中绿原酸及橙皮苷含量的检测方法。 方法 高效液相色谱法,色谱柱为C18柱(150 mm×4.6 mm,5 μm),以乙腈为流动相A,0.1%磷酸为流动相B,梯度洗脱,检测波长为290 nm;流速为1.0 ml/min。 结果 绿原酸对照品在4.040~80.80 μg/ml 范围内相关系数r=1.000 0;橙皮苷对照品在3.618~72.35 μg/ml 范围内相关系数r=1.000 0,绿原酸平均加样回收率为100.2%,RSD为0.83%;橙皮苷平均加样回收率为99.95%,RSD为0.61%。 结论 本方法简单准确、重现性好,专属性强,适用于利湿口服液中上述成分含量的检测。
Abstract:
Objective To establish a method for determining chlorogenic acid and hesheridin in Lishi Oral Liquid (Plantaginis Herba, Kochiate Frouctus, Lonicerae Japonicae Flos, Sophorpe Flavescentis Radix, Citri Reticulatae Pericarpium, Poriae Cutis, Maifahitum) by HPLC. Methods The HPLC with a C18 column was used.The mobile phases was a mixture of acetonitrile for current phase A, 1% Phosphoric acid solution for current phase B; the form prescribed in the gradient elution. The detection wavelength was 290 nm, flow velocity was 1.0 ml/min. Results The linear range was 4.040~80.80 μg/ml for chlorogenic acid and 3.618~72, 35 μg/ml for hesperidin.The average recovery of chlorogenic acid was 100.2% (RSD was 0.83%). The average recovery of hesperidin was 99.95%(RSD was 0.61%). Conclusions The method was simple, accurate, and reproducible with exclusive propertywhich could be used for quality control of Lishi Oral Liquid.
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