Determination of quercetin in Gnaphalium affine
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摘要: 目的 建立鼠曲草中槲皮素的含量测定方法。 方法 采用VP-ODS C18(4.6 mm×150 mm, 5 μm)色谱柱。以甲醇-0.2%磷酸溶液(45:55)为流动相;流速为1.0 ml/min;检测波长为375 nm。 结果 鼠曲草中的槲皮素含量测定方法在10.22~51.10 μg/ml浓度范围内与峰面积的线性关系良好(r=0.999 7);平均回收率为100.18%,RSD为1.38%。 结论 所建立的方法准确、灵敏、重现性好,可用于鼠曲草的质量控制。Abstract: Objective To establish the determination method of Quercetin content in Gnaphalium affine D. Don. Methods VP-ODS Cl8(4.6 mm×150 mm, 5 μm) colmnn was used, the mobile phase was methanol-0.2% phosphoric acid solution(45:55) with the flow rate as 1.0 ml/min. The detection wavelength was 375 nm. Results The linear relation of hyperin was good in the range from 10.22 μg/ml to 51.10 μg/ml. The average recovery was 100.18%, RSD was 1.38%. Conclusion The method established was accurate, sensitive and good in reproducibility which could be applied to the quality control of G. affine D. Don.
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Key words:
- Gnaphalium affine /
- quercetin /
- HPLC
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[1] 《全国中草药汇编》编写组.全国中草药汇编:上册[M].北京:人民卫生出版社,1996:894. [2] 国家中医药管理局,《中华本草》编委会.中华本草:第7册[M].上海:上海科学技术出版社,1999:848. [3] 李少锋,崔桂友.高效液相色谱法测定丁香中槲皮素的含量[J].扬州大学烹饪学报,2011, 28(1):51. [4] 王 丽,吕圭源,王 利.HPLC测定乌药叶中槲皮素的含量[J].北京联合大学学报:自然科学,2011,25(2):50. -
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