摘要:
目的 建立并优化一种简单、快速、灵敏及高选择性测定氟西汀血药浓度的高效液相色谱法(HPLC),用于临床个体化给药监测。 方法 血浆样品用乙腈沉淀蛋白法处理。样品分离用Hypersil ODS2 (5 μm, 4.6 mm×200 mm)色谱柱,流动相为乙腈-25 mmol/L磷酸盐缓冲液(pH6.5)(34:66),流速为1.2 ml/min;进样量为20 μl,紫外检测波长227 nm。 结果 氟西汀的血浆浓度范围是0.5~50 ng/ml,r=0.996 9,低、中、高血浆质控样品日
Abstract:
Objective To establish and optimize a simple, rapid, reliable, high sensitive and high selective method for the determination of fluoxetine hydrochloride in plasma by high-performance liquid chromatographic (HPLC) so as to monitor the clinical drug use. Methods The plasma samples were deproteined by acetonitrile. Separation was performed on a Hypersil ODS2 column(5 μm, 4.6 mm×200 mm). The mobile phase contained acetonitrile-25 mmol/ L phosphate buffer(pH6.5)(34:66). The flow rate and sample volume injected were 1.2 ml/min and 20 μl, respectively. Detection wavelength was 227 nm. Results The calibration curve of fluoxetine hydrochloride in plasma was linear in the concentration range of 0.5~50 ng/ml and coefficient was 0.996 9. The intra-assay precision did not exceed 8.06% and inter-assay precision did not exceed 10.46% for low, medium and high quality samples, respectively. The average recovery of the described method was 91.7%,97.06% and 100.75%, respectively. Conclusion In comparison with previous work, the improved chromatographic method was more economical, less polluted and better protected column, besides simple preparation of plasma samples, high reproducibility, and high selectivity.
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