摘要:
目的 建立RP-HPLC法同时测定藏药螃蟹甲中3个苯乙醇苷的含量。 方法 色谱柱为Symmetry C18 (4.6 mm ×150 mm,5 μm),流动相为甲醇-水(36:64),冰醋酸调pH至4.5;流速1.0 ml/min;柱温20 ℃,检测波长330 nm。 结果 毛蕊花糖苷、alyssonoside、异毛蕊花糖苷分别在20~500(r=0.999 6), 10~130(r=0.999 5), 10~130 (r=0.999 7) μg/ml范围间与峰面积线性关系良好;平均加样回收率均在96%~104%之间,RSD均小于5%(n=6)。 结论 该方法专属性强、灵敏度高、精确度与重现性好,可作为螃蟹甲药材的质量控制方法。
Abstract:
Objective To establish a HPLC method for the determination of three phenylethanoid glycosides in Phlomis younghusbandii. Methods HPLC analysis was performed on Symmetry C18 (4.6 mm×150 mm, 5 μm) column and the mixture of methanol-water (36:64, pH=4.5, HAc) was used as the mobile phase with a flow rate of 1.0 ml/min. The column temperature was 20 ℃ and the detection wavelength was 330 nm. Results The linear ranges of acteoside,alyssonoside,isoacteoside were 20~500(r=0.999 6), 10~130(r=0.999 5), 10~130 (r=0.999 7) μg/ml, respectively. The average recoveries (n=6) of them were all between 96% and 104%, RSD<5%. Conclusions This method was special, sensitive, accurate and with good repeatability, which could be used for quality control of Phlomis younghusbandii.
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