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在线样品预处理限进色谱测定人血清中多奈哌齐浓度

马骏,  樊鹏程,  任俊,  贾正平

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文章导航 > 药学实践与服务  > 2011 >  29(2): 122-124
马骏, 樊鹏程, 任俊, 贾正平. 在线样品预处理限进色谱测定人血清中多奈哌齐浓度[J]. 药学实践与服务, 2011, 29(2): 122-124.
引用本文: 马骏, 樊鹏程, 任俊, 贾正平. 在线样品预处理限进色谱测定人血清中多奈哌齐浓度[J]. 药学实践与服务, 2011, 29(2): 122-124.
shu
MA Jun, FAN Peng-cheng, REN Jun, JIA Zheng-ping. Detection of donepezil from serum by on-line sample preparation RAM-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 122-124.
Citation: MA Jun, FAN Peng-cheng, REN Jun, JIA Zheng-ping. Detection of donepezil from serum by on-line sample preparation RAM-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 122-124.
shu

在线样品预处理限进色谱测定人血清中多奈哌齐浓度

  • 兰州军区兰州总医院药剂科,甘肃 兰州 730050

Detection of donepezil from serum by on-line sample preparation RAM-HPLC

  • Department of Pharmacy, General Hospital of Lanzhou Command, Lanzhou 730050,China

  • 摘要: 目的 通过在线样品预处理限进色谱柱切换技术建立一种测定人体血清中多奈哌齐血药浓度的高效液相色谱法。 方法 样品预处理采用LiChroCART (25 mm × 4 mm,25 μm) 限进预柱,分析柱为Microsorb-MV C18柱 (150 mm × 4.6 mm,4 μm ),以水-乙腈 (991, v/v) 为血浆蛋白冲洗流动相,流速2 ml/min,0.02 M 磷酸缓冲液-乙腈-三乙胺 (65350.1, v/v/v) 为分析流动相,流速1 ml/min,检测波长315 nm。 结果 本方法线性范围为0.02~1.0 μg/ml,r=0.999 5,日内、日间精密度RSD小于7%,回收率为87.9%~91.1%。 结论 该方法准确、稳定;灵敏度高,适用于多奈哌齐药物动力学及生物利用度的研究。
    Abstract: Objective To establish a sample preparation restricted access material (RAM) HPLC method for the quantification of donepezil in human serum. Method The sample was prepocessed by LiChrospher ADS C18 (25 mm × 4 mm,25 μm) as pre-column and analyzed by Microsorb-MV C18 (150 mm × 4.6 mm,4 μm ) as analytical column. Acetonitrile to water (991, v/v) was flushing mobile phase with flow rate of 2 ml/min. The mixture of phosphate buffer (pH 4.6, 0.02 M), acetonitril and triethylamine (65350.1, v/v/v) was analytical mobile phase with flow rate of 1 ml/min. The detection wave was 315 nm. Results The linear range was from 0.02 to 1.0 μg/ml (r=0.999 5). The RSDs of intra-day and inter-day were less than 7%, the recoveries were between 87.9%~91.1%. Conclusion The method is accurate, stable and sensitive which could be used to study the pharmacodynamics and bioavailabilty of donepezil.
  • [1] Rogers SL, Farlow MR, Doody RS, et al. A 24-week, double-blind, placebo-controlled trial of donepezll in patients with Alzheimer 's disease[J]. Neurology, 1998, 50: 136.
    [2] Norio YF, Furuya R, Takahata T, et al. Determination of donepezil, an acetylcholinesterase inhibitor, in human plasma by high-performance liquid chromatography with ultraviolet absorbance detection[J]. J Chromatogr B, 2002, 768: 261.
    [3] Radwan MA, Abdine HH, Al-Quadeb BT, et al. Stereoselective HPLC assay of donepezil enantiomers with UV detection and its application to pharmacokinetics in rats[J]. J Chromatogr B, 2006, 830: 114.
    [4] Nakashima K, Itoh K, Kono M, et al. Determination of donepezil hydrochloride in human and rat plasma, blood and brain microdialysates by HPLC with a short C30 column[J]. J Pharm Biomed Anal, 2006, 41:201.
    [5] Souverain S, Rudaz S, Veuthey JL, et al. Restricted access materials and large particle supports for on-line sample preparation: an attractive approach for biological fluids analysis[J]. J Chromatogr B, 2004, 801:141.
    [6] Chiap P, Piette M, Evrard B, et al. Automated method for the determination of a new matrix metalloproteinase inhibitor in ovine plasma and serum by coupling of restricted access material for on-line sample clean-up to liquid chromatography[J]. J Chromatogr B, 2005, 817:109.
    [7] Rbeida O, Chiap P, Lubda D, et al. Development and validation of a fully automated LC method for the determination of cloxacillin in human plasma using anion exchange restricted access material for sample clean-up[J]. J Pharm Biomed Anal, 2005, 36: 961.
    [8] Vintiloiu A, Mullett WM, Papp R, et al. Combining restricted access material (RAM) and turbulent flow for the rapid on-line extraction of the cyclooxygenase-2 inhibitor rofecoxib in plasma samples[J]. J Chromatogr A, 2005, 1082:150.
    [9] Musteata FM, Walles M, Pawliszyn J. Fast assay of angiotensin 1 from whole blood by cation-exchange restricted-access solid-phase microextraction[J]. Anal Chim Acta, 2005, 537: 231.
    [10] Lambert JP, Mullett WM, Kwong E, et al. Stir bar sorptive extraction based on restricted access material for the direct extraction of caffeine and metabolites in biological fluids[J]. J Chromatogr A, 2005, 1075: 43.
    [11] Neto AJ, Rodrigues JC, Fernandes C, et al. Automated microcolumn-switching system for drug analysis by direct injection of human plasma[J]. J Chromatogr A, 2006, 1105:71.
    [12] Ortelli D, Rudaz S, Souverain S, et al. Restricted access materials for fast analysis of methadone in serum with liquid chromatography-mass spectrometry[J]. J Sep Sci, 2002, 25:222.
    [13] 樊鹏程, 马 骏, 贾正平, 等. 在线柱切换RP-HPLC测定人血清中卡马西平浓度[J].药物分析杂志, 2008, 28(12): 2033.
    [14] Yamamoto E, Igarashi H, Sato Y, et al. Reliable on-line sample preparation of basic compounds from plasma using a reversed phase restricted access media in column-switching LC[J]. J Pharm Biomed Anal, 2006, 42:587.
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    [16] Asakawa Y, Ozawa C, Osada K, et al. Reduction of carry-over in column-switching HPLC/MS system with automated system washing procedure for highly sensitive direct analysis of donepezil in dog plasma[J]. J Pharm Biomed Anal, 2007, 43:683.
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出版历程
  • 收稿日期:  2009-12-10
  • 修回日期:  2010-10-02

在线样品预处理限进色谱测定人血清中多奈哌齐浓度

  • 兰州军区兰州总医院药剂科,甘肃 兰州 730050
  • 收稿日期: 2009-12-10
  • 修回日期: 2010-10-02

摘要: 目的 通过在线样品预处理限进色谱柱切换技术建立一种测定人体血清中多奈哌齐血药浓度的高效液相色谱法。 方法 样品预处理采用LiChroCART (25 mm × 4 mm,25 μm) 限进预柱,分析柱为Microsorb-MV C18柱 (150 mm × 4.6 mm,4 μm ),以水-乙腈 (991, v/v) 为血浆蛋白冲洗流动相,流速2 ml/min,0.02 M 磷酸缓冲液-乙腈-三乙胺 (65350.1, v/v/v) 为分析流动相,流速1 ml/min,检测波长315 nm。 结果 本方法线性范围为0.02~1.0 μg/ml,r=0.999 5,日内、日间精密度RSD小于7%,回收率为87.9%~91.1%。 结论 该方法准确、稳定;灵敏度高,适用于多奈哌齐药物动力学及生物利用度的研究。

English Abstract

马骏, 樊鹏程, 任俊, 贾正平. 在线样品预处理限进色谱测定人血清中多奈哌齐浓度[J]. 药学实践与服务, 2011, 29(2): 122-124.
引用本文: 马骏, 樊鹏程, 任俊, 贾正平. 在线样品预处理限进色谱测定人血清中多奈哌齐浓度[J]. 药学实践与服务, 2011, 29(2): 122-124.
shu
MA Jun, FAN Peng-cheng, REN Jun, JIA Zheng-ping. Detection of donepezil from serum by on-line sample preparation RAM-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 122-124.
Citation: MA Jun, FAN Peng-cheng, REN Jun, JIA Zheng-ping. Detection of donepezil from serum by on-line sample preparation RAM-HPLC[J]. Journal of Pharmaceutical Practice and Service, 2011, 29(2): 122-124.
shu

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