摘要:
目的: 采用液质联用方法测定白色念珠菌中海藻糖的含量,考察白色念珠菌在感应分子法尼醇作用后海藻糖的变化。 方法: 色谱柱:Shisheido CAPCELL NH2（150 mm×4.6 mm,5μm）,柱温:25℃,流动相:乙腈-水溶液（75:25,V/V）,流速:1ml/min,分流比3:1。质谱检测器,ESI（-）模式,选择离子检测,碎片电压120 V。 结果: 海藻糖和内标峰形均良好,海藻糖含量在0.05-50μg/ml范围内线性关系良好,r=0.999 3。日内日间精密度RSD值分别为1.8%和2.1%。 结论: 该方法简便易行,选择内标之后含量测定更精确,灵敏度提高。
Abstract:
Objective To establish a high performance liquid chromatography-mass spectrum(HPLC-MS) method to determine trehalose in the biofilm of Candida albicans. Methods Shisheido CAPCELL NH2(150 mm×4.6 mm,5 μm) was used in this method and the mobile phase is 75% acetonitrile and 25% pure water.MS detector with an ESI ion source in negative mode was used. Results It has a good separation of trehalose and internal standard,and there is no interference of endogenous impurity.Linear was from 0.05～50 μg/ml(r=0.999 3),recovery was in the range of 81.50% to 96.29% and the RSD of precision is less than 2.1 %. Conclusion This method is a simple method for quantification of trehalose in fungus.
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