摘要:
目的建立高效液相色谱法同时测定七味葡萄散中桂皮醛和甘草酸的含量。方法色谱柱为Agilent ZORBAX E-clipse SB-C18(4.6@150 mm,5Lm);流动相为乙腈-5%乙腈(含3%冰醋酸)(27:73);流速为1 ml/min;检测波长为250 nm。结果桂皮醛和甘草酸保留时间分别约为10.2和20.7 min,与各自相邻峰的分离度均大于1.5。以峰面积对进样浓度(μg/ml)线性回归,桂皮醛回归方程为Y=0.083 05X-1.205,r=0.999 9,线性范围19.40~242.5μg/ml;甘草酸回归方程为Y=0.142 0X+1.340,r=0.999 9,线性范围12.48~156.0μg/ml;桂皮醛和甘草酸的回收率分别为100.2%和100.5%,RSD分别为1.5%和1.9%。结论本方法操作简便,测定结果准确,重复性好,可用于七味葡萄散中桂皮醛和甘草酸的含量测定。
Abstract:
Objective To establish an HPLC quantitative method for the determination of cinnamaldehyde and glycyrrhizic acid in Qiwei Putao power simultaneously. Methods The chromatographic conditions include column C18(Agilent ZORBAX Eclipse SB-C18,4.6@150 mm,5 Lm),acetonitrile25% acetonitrile(include 3% glacialacetic acid)(27z73) as mobile phase.The flow rate was 1 ml/min and monitored at 250 nm. Results The retention time of cinnamaldehyde and glycyrrhizic acid was about 10.2 min and 20.7 min respectively.The resolution was more than 1.5.The regress equation for cinnamaldehyde was Y=0.083 05X-1.205,r=0.999 9,and the linear range was 19.40 ～242.5 μg /ml.glycyrrhizic acid was Y=0.142 0X +1.340,r=0.999 9,and the linear range was 12.48 ～156.0μg/ml.The average recovery of cinnamaldehyde and glycyrrhizic acid was 100.2% and 100.5%,RSD 1.5% and 1.9% respectively. Conclusion This method is simple,time-saving and accurate.It can be used for routine analysis of cinnamaldehyde and glycyrrhizic acid in Qiwei Putao power.
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