HPLC法测定林可霉素乳膏中盐酸林可霉素的含量
Determination of lincomycin hydrochloride in lincomycin cream by HPLC
-
摘要: 目的: 建立高效液相色谱法测定林可霉素乳膏中盐酸林可霉素的含量。 方法: 以YMC C18（250 mm×4.6 mm,5μm）为色谱柱,0.05 mol/L硼砂溶液（磷酸溶液调节pH值至6.0）-甲醇（55:45）为流动相,紫外检测波长为214 nm。 结果: 盐酸林可霉素在10～30μg范围内线性关系良好（r=0.999 7）,平均回收率为98.74%,（RSD=2.06%,n=9）。3批林可霉素乳膏中盐酸林可霉素的标示含量分别为96.21%、96.50%、93.38%。 结论: 本方法操作简便、快速,结果准确可靠,适用于林可霉素乳膏的质量控制。Abstract: Objective:To establish high performance liquid chromatography method for determination the content of lincomycin hydrochloride in lincomycin cream.Methods:A YMC C18（250 mm×4.6 mm,5μm）column was used with the mobile phase of 0.05mol/L borax （adjust pH to 6.0 with phosphoric acid）-methanol （55:45）and UV wavelengh of 214 nm.Results:The calibration curve of lincomycin hydrochloride was line in the range of 10～30μg（r=0.999 7）.The average recovery was 98.74%（RSD= 2.06%,n=9）.The relative content of lincomycin hydrochloride in three batch of lincomycin cream was 96.21%,96.50% and 93.38%.Conclusion:This method is simple,quick,accurate and suitable for the quality control of lincomyein cream.
-
Key words:
- lincomycin hydrochloride /
- cream /
- HPLC
-
点击查看大图
计量
- 文章访问数: 2474
- HTML全文浏览量: 181
- PDF下载量: 416
- 被引次数: 0
下载:
