摘要:
目的: 对3种粒径的黄芪,采用4种不同的方法提取黄芪多糖及黄芪总皂苷,优选最佳粒径和提取方法。 方法: 对超微粉、200目细粉、饮片3种粒径的黄芪,分别采用水醇双提法、纤维素酶法、氧化钙溶液法、超声波法进行提取,用超滤法与D21大孔吸附树脂法进行分离,分别比较黄芪多糖及黄芪总皂苷的得率。 结果: 黄芪超微粉用超声波法提取黄芪多糖得率最高,达到1.73%;采用黄芪饮片用超声波法提取黄芪总皂苷得率最高,达到1.82%。 结论: 超声波提取方法对提取黄芪多糖和黄芪总皂苷的得率较其它方法都有较大的提高。黄芪的粒径越小对黄芪多糖提取得率越高,但对黄芪总皂苷的提取,随粒径减小而得率减少。
Abstract:
Objective: To select optimal diameter of the powder of Radix Astragali and extraction method by four methods of extracting astragalus polysaccharide and total astragalus saponins in three Radix Astragali powders of different diameter. Methods: The water-alcohol co-distillation, cellulose degradation technology, calcarea solution technology, ultrasonic waves technology were used to three Radix Astragali powders of different diameter respectively.The astragalus polysaccharide and total astragalus saponins were separated with combined techniques hollow fiber membrane and D21 macro absorbing resin. According to the contents of astragalus polysaccharide and total astragalus saponins,the extraction methods were compared. Results: The most effective way of extracting astragalus polysaccharide was the ultrasonic waves technology with the yield rate of 1.73%, while the most effective method of distilling total astragalus saponins was ultrasonic waves technology on prepared slice of herbal drug, with the yield of 1.82. Conclusion: Ultrasonic waves technology was a much better way of extracting both astragalus polysaccharide and total astragalus saponins. The shorter the diameter of Radix Astragali powder, the higher the yield of extracting astragalus polysaccharide. The yield of extracting total astragalus saponins turned out contray.
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