摘要:
目的:建立测定五味子配方颗粒中主要木脂素类成分（五味子醇甲、五味子酯甲、五味子甲素、五味子乙素）含量的方法,分析不同厂家的五味子配方颗粒中木脂素成分的差异。方法:采用RP-HPLC法,并结合二极管阵列检测器（PDA）进行光谱分析。色谱柱:Hypersil ODS C18柱（4.6 mm×250 mm,10μm）,流动相:A为乙腈,B为水,梯度洗脱,流速1.0 mL/min,柱温25℃,检测波长254 nm。结果:不同厂家五味子配方颗粒中五味子醇甲含量为0-1.292 3 mg/包、五味子酯甲含量为0-0.560 8 mg/包、五味子甲素含量为0-0.168 8 mg/包、五味子乙素均未检测出。结论:不同厂家产品五味子醇甲、五味子酯甲、五味子甲素含量差异显著。
Abstract:
Objective :To establish a method to determine the active components of lignans(schisandrin,schisandtherin A,deoxyschizandrin and schisandrin B) in decoction granule of Schisandra chinensis.To find and anylyse the difference of components of different factories. Methods :RP-HPLC method was used.The column was Hypersil ODS C18(4.6 mm×250 mm,10 μm) with the mobile phase of acetonititrile(A) and H2O(B),the flow rate was 1.0 mL/min,the column temperature was set at 25 ℃ and the detection wavelength was set at 254 nm. Results : The content of schisandrin was from 0 to 1.292 3mg/package,schisandtherin A was from 0 to 0.560 8 mg/package,deoxyschizandrin was from 0 to 0.168 8mg/package.Schisandrin B was not detected. Conclusion : The content of schisandrin,schisandtherin A and deoxyschizandrin were obviously different in decoction granule of Schisandra chinensis produced by different factories.
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