摘要:
目的: 对牡丹皮中丹皮酚的提取工艺进行研究,考察超声辅助水蒸气蒸馏提取的效果,改进提取工艺。 方法: 采用中国药典HPLC方法测定丹皮酚的含量,系统比较蒸馏水用量、乙醇加入量和超声时间等3个主要因素,计算馏出液中丹皮酚的提取结果。 结果: 确定水蒸气蒸馏提取牡丹皮中丹皮酚的工艺为药材加10倍量蒸馏水,超声20 min后,蒸馏,收集至蒸馏液不再浑浊时为止。 结论: 本工艺采用超声对药材进行预处理,可以极大的提高丹皮酚的提取效率,操作简单、成本低、毒性小,可用于丹皮酚的大规模制备。
Abstract:
Objective:To study the extraction technology of paeonol from Cortex Moutan,and improve the extraction method of steam distillation with ultrasonic assistance. Methods: The paeonol was determined by HPLC method in Chinese Pharmacopeia 2005. The distilled water amount,alcohol addition amount and uhransonic time,meanwhile were conpared,the extraction amounts of paeonol in distillate were calculated. Results: The final affirmed method of paeonol extraction was ten times distilled water and transonic twenty minutes,then distill until the distillate was not turbid. Conclusion: The final method was pretreated by ultrasonic which could increase the extraction efficiency greatly,and has the advantages of simple,economic and low toxicity,also can be used in the large-scale prep- aration of paeonol.
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