摘要:
目的： 建立一种反相高效液相色谱分析方法,同时测定小柴胡汤中黄芩苷和甘草酸的含量。 方法： 采用Agilent Zorbax SB C18柱（4．6mm×250mm,5m）,以乙腈-0．01%磷酸溶液为流动相,梯度洗脱,流速1．0mL/min．柱温25℃,检测波长为250nm。 结果： 黄芩苷和甘草酸单铵盐在50min内即实现良好分离,黄芩苷浓度在12．66～405．0g/mL线性范围内呈良好的线性关系,其回归方程为：Y=19．726X+13．972,r= 0．9998。甘草酸在1．988～63．60g/mL的线性范围内呈良好的线性关系,其回归方程为：Y=10．765X+ 6．6433,r=0．9997。两种成分精密度试验RSD＜1%；48h内稳定性试验RSD＜1%；加样回收率试验RSD＜2%。 结论： 该方法简便快速、准确可靠,能同时测定小柴胡汤中黄芩苷及甘草酸两种有效成分的含量,为有效控制小柴胡汤的质量提供参考。
Abstract:
Objective: To establish a method for simultaneous determination of baicalin,glycyrrbizic acid in Xiaochaihu decoction by RP-HPLC. Methods: A Agilent Zorbax SB C18（4.6mm×250mm,5m） was used with a mobile phase of acetonitrile-0.01% H3 PO4 in gradient elution,The flow rate was 1.0mL/min and the detection wavelength was set at 250nm. Results: The two constituents were separated within 50 min.The linearity was obtained over 12.66～405.0g/mL（r=0.9998）for baicalin,1.988～63.60 g/mL（r =0.9997） for glycyrrhizic acid respectively.The RSD of precision and stability of the sample was less than 1% in 48 hours.The RSD of the average recovery was less than 2%. Conclusion: The method is rapid,simple and accurate,and it can be suitable for the simulta- neous determination of baicalin,glycyrrhizic acid in Xiaochaihu decoction and the quality control of Xiaochaihu decoction.
点击查看大图
下载:
