摘要:
目的 测定知母药材中芒果苷和新芒果苷的含量.方法 采用HPLC法,色谱柱为ZorbaxEclipseXDB-C18柱(5μm,4.6×250mm,Agilent),流动相为乙腈和25mmol/L磷酸二氢钾缓冲液,梯度洗脱;流速:1.0mL/min,运行时间60min,检测波长257nm.结果 芒果苷在14.2-568.5μg/mL范围内线性关系良好(r=0.9999),高中低浓度日内精密度RSD在2.4%-3.3%之间,日间精密度RSD在2.5%-4.5%之间;新芒果苷在14.8-590.5μg/mL范围内线性关系良好(r=0.9999),高中低浓度日内精密度RSD在1.9%-3.4%之间,日间精密度RSD在1.5%-3.9%之间.结论 本方法准确可靠,重现性好,可用作知母药材中芒果苷和新芒果苷的含量测定,为知母药材质量标准提供参考.
Abstract:
Objective To establish the method for determination of mangiferin and neo-mangiferin in Rhizoma Anemarrhenae. Method The content was determined by HPLC. A Zorbax Eclipse XDB-C18 column(4.6mm×250mm,5μm) with a grient mobile phase composed of 25 mmol/L dihydrophosphate potassium and acetonitrile were used. The detection wavelength was 257 nm and the flow rate was 1.0mL/min. Results The calibration curves of mangiferin were linear between 14.2-568.5μg/mL(r=0.9999), the neo-mangiferin were 14.8-590.5μg/mL. The withinday precision RSD was both less than 4.5% and the interday precision RSD was less than 3.9%. Conclusion The method is simple, rapid, accurate and is suitable for the determination of mangiferin and neo-mangiferin.
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