摘要:
目的 优选鼻窦炎滴丸提取条件。方法 采用正交试验法,以黄芪甲苷的提取率为主要评价指标,考察加水量、提取次数、醇沉浓度、煎煮时间等四个条件的影响,用HPLC法测定黄芪甲苷含量,采用DELTA-PAKC18柱,流动相为乙腈-0.1%磷酸溶液(33:67),在201nm紫外光下检测。结果 回归方程A=69633.018V+1290.434,r=0.9998,线性范围为0.044-0.440mg/mL,平均加样回收率为92.58%,RSD值为1.50%(n=5)。结论 实验结果表明,该方法灵敏准确、专属性强,结果可靠、稳定、重现性好、精密度高,可作为鼻窦炎滴丸的质量控制标准,并且达到了用正交法筛选最佳工艺流程的目的。
关键词:
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滴丸 /
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黄芪 /
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正交试验 /
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黄芪甲苷
Abstract:
Objective To choice the distilling condition of astragaloside in nasal sinusitis dripping pills(NSDP). Methods In-vestigating the influence of the four condition (including water volume, distilling times, concentration for ethanel precipitation and time of decoction) by adopting orthogonal design method, using the withdraw rate of astragaloside A as a main evaluation standard. The content of astragaloside A in NSDP was determined by HPLC. The mobile phase was acetonitrile:0.1% phosphoric acid(33:67)and detected on DELTA-PAK C18 column at the wavelength of 201nm. ResultsThe regression equation within the spotted range of 0.044-0.440mg/mL was A=69633.018V+1290.434 with good linearity, r=0.9998. The average recovery rate was 92.58% and RSD=1.50%(n = 5). Conclusion The experiment result prove that the way is intelligent, accurate, specific, reliable, stability, precise, which is suitable for the quality control of NSDP. And we obtain the best technical process of NSDP with orthogonal design.
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