摘要:
目的 建立薏苡仁的指纹图谱分析方法,应用于实际工作中。方法 采用HPLC-ELSD法对薏苡仁中甘油三酯类成分进行指纹分析,Discovery® C18为色谱柱,流动相为乙腈-二氯甲烷(59:41),流速为每分钟0.5mL,漂移管温度为70℃,氮气流速为每分钟1.2L。结果 指纹图谱中共有7个主要色谱峰,其相对保留时间的重现性RSD均小于0.3%,精密度RSD均小于0.1%,相对峰面积的重现性RSD均小于1.8%,精密度RSD均小于2.0%,不同产地的薏苡仁指纹图谱稳定,重现性好。结论 该指纹图谱方法可靠、简便,可作为薏苡仁质量控制指标。
Abstract:
OBJECTIVE To establish the fingerprint method for semen coicis. METHODS The Discovery® C18column with acetonitrile-ichloromethanol(59:41)as the mobile phase was used in HPLC-ELSD. The flow rate was 0.5mL/min and ELSD tube temperature is 70℃. The flow rate of Nitrogen is 1.2L/min. RESULTS:There are 7 mostly chromatogram in the fingerprint of semen coicis,the relative standard deviation(RSD)of relative retain time for reproducibility was not more than 0.3%,for precision was not more than 0.1%.the relative standand deviation(RSD)of relative peak area for reproducibility was not more than 1.8%,for precision was not more than 2.0%,and the fingerprint for semen coicis of different producing area is very stabilization. CONCLUSION The method is simple,accurate with a good reproducibility and can be used as a quantitative analysis method for semen coicis.
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