摘要:
采用RP－HPLC法测定注射用卡铂的含量,色谱柱：YWG－C18柱,流动相为纯水,检测波长210nm,用尿苷作内标。在上述条件下,卡铂和尿苷的保留时间分别为6.5min和10.5min且分离良好(R>1.5),方法专属性强,操作简便,结果准确。日内和日间RSD分别为2.35%(n＝5)和3.56%(n＝5)。稳定性试验结果表明,注射用卡铂在室温(＜40℃)和冷藏(4℃－10℃)避光条件下存放2a,其含量基本保持不变。
Abstract:
A RP-HPLC method for the quantitative determination of carboplation injection has been developed by using YWG-C18 column, using pure water as mobile phase and deteced at 210nm. The uridine was used as internal standard. Under these condition, the retention of carboplatin and uridine was 6.5 and 10.5 minture,respectively (R > 1 .5). The method is simple,rapid and sensitive. The within-day and between-day precisions were 2. 35% (n = 5) and 3. 56% (n = 5) respectively. The stability test showed the carboplatin injection store at room tempoerature (< 40℃) and store in refrigerator(4℃-10℃)for two years, and kept away from light, the contents of carboplatin injection were not significantly changed.
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